PROM2 Antibody - #DF14670

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製品: PROM2 Antibody
カタログ: DF14670
タンパク質の説明: Rabbit polyclonal antibody to PROM2
アプリケーション: IHC
Cited expt.: IHC
反応性: Human, Mouse, Rat
分子量: 92kD(Calculated).
ユニプロット: Q8N271

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
PROM2 Antibody detects endogenous levels of PROM2.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

hPROML2; MGC138714; OTTHUMP00000160939; OTTHUMP00000207357; PROM 2; PROM-2; PROM2; PROM2_HUMAN; Prominin like protein 2; Prominin related protein; Prominin-2; Prominin-like protein 2; PROML2;

免疫原

免疫原:

A synthesized peptide derived from human PROM2.

Uniprot:

Q8N271(PROM2_HUMAN) >>Visit HPA database.

遺伝子(ID):
PROM2(150696)
発現特異性:
Q8N271 PROM2_HUMAN:

Present in saliva within small membrane particles (at protein level). Expressed in kidney, prostate, trachea, esophagus, salivary gland, thyroid gland, mammary gland adrenal gland, placenta, stomach, spinal cord and liver. In submucosal tumor, expressed in spindle-shaped or stellate stromal cells. Expressed in prostate cancer cell lines.

タンパク質配列:
MKHTLALLAPLLGLGLGLALSQLAAGATDCKFLGPAEHLTFTPAARARWLAPRVRAPGLLDSLYGTVRRFLSVVQLNPFPSELVKALLNELASVKVNEVVRYEAGYVVCAVIAGLYLLLVPTAGLCFCCCRCHRRCGGRVKTEHKALACERAALMVFLLLTTLLLLIGVVCAFVTNQRTHEQMGPSIEAMPETLLSLWGLVSDVPQELQAVAQQFSLPQEQVSEELDGVGVSIGSAIHTQLRSSVYPLLAAVGSLGQVLQVSVHHLQTLNATVVELQAGQQDLEPAIREHRDRLLELLQEARCQGDCAGALSWARTLELGADFSQVPSVDHVLHQLKGVPEANFSSMVQEENSTFNALPALAAMQTSSVVQELKKAVAQQPEGVRTLAEGFPGLEAASRWAQALQEVEESSRPYLQEVQRYETYRWIVGCVLCSVVLFVVLCNLLGLNLGIWGLSARDDPSHPEAKGEAGARFLMAGVGLSFLFAAPLILLVFATFLVGGNVQTLVCQSWENGELFEFADTPGNLPPSMNLSQLLGLRKNISIHQAYQQCKEGAALWTVLQLNDSYDLEEHLDINQYTNKLRQELQSLKVDTQSLDLLSSAARRDLEALQSSGLQRIHYPDFLVQIQRPVVKTSMEQLAQELQGLAQAQDNSVLGQRLQEEAQGLRNLHQEKVVPQQSLVAKLNLSVRALESSAPNLQLETSDVLANVTYLKGELPAWAARILRNVSECFLAREMGYFSQYVAWVREEVTQRIATCQPLSGALDNSRVILCDMMADPWNAFWFCLAWCTFFLIPSIIFAVKTSKYFRPIRKRLSSTSSEETQLFHIPRVTSLKL

研究背景

PTMs:

Glycosylated.

細胞の位置付け:

Apical cell membrane>Multi-pass membrane protein. Basolateral cell membrane>Multi-pass membrane protein. Cell projection>Microvillus membrane>Multi-pass membrane protein. Cell projection>Cilium membrane>Multi-pass membrane protein.
Note: Colocalizes with PROM1. Associates with membrane in a cholesterol-dependent manner. Localizes to the apical and basolateral membranes of epithelial cells (By similarity).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Present in saliva within small membrane particles (at protein level). Expressed in kidney, prostate, trachea, esophagus, salivary gland, thyroid gland, mammary gland adrenal gland, placenta, stomach, spinal cord and liver. In submucosal tumor, expressed in spindle-shaped or stellate stromal cells. Expressed in prostate cancer cell lines.

タンパク質ファミリー:

Belongs to the prominin family.

参考文献

1). Inhibition of HOXC11 by artesunate induces ferroptosis and suppresses ovarian cancer progression through transcriptional regulation of the PROM2/PI3K/AKT pathway. World journal of surgical oncology, 2024 (PubMed: 39380001) [IF=3.2]

Application: WB    Species: Mouse    Sample:

Fig. 1 Identification of ferroptosis-related genes induced by artesunate (ART) by RNA-seq (A) Volcano plot showed differentially expressed genes (DEGs) between control and ART-treated ES2 cells (|log2FC| > 1 and p value 

Application: IHC    Species: Mouse    Sample:

Fig. 7 Artesunate (ART) induced ferroptosis-mediated tumor inhibition by suppressing the HOXC11/PROM2/PI3K/AKT pathway in a SKOV3 xenograft mouse model (A) Scheme of the experimental design and timeline for the tumor model. SKOV3 cells (5 × 106 cells for each mouse) were inoculated into BALB/c-nude mice subcutaneously. Tumor-bearing mice were treated with vehicle (5% DMSO) or ART at 70 mg/kg daily for 25 days (n = 6 for each group). Body weights and tumor volumes were monitored every three days. B Images of xenograft nude mice and tumor mass. C Curve of tumor volume. D Curve of body weight. E Tumor weight. The superoxide dismutase (SOD) activity (F), glutathione (GSH) levels (G) and malondialdehyde (MDA) levels (H) were measured in the tumor tissue of nude mice. I IHC staining was performed to detect the expression of HOXC11, PROM2 and GPX4 in the tumor tissues of xenografted mice. J Western blot analysis of HOXC11, PROM2, GPX4, p-PI3K, PI3KCG, p-AKT and AKT in tumor tissue samples obtained from xenograft mice treated with vehicle or ART. GAPDH was used as a loading control. Data were analyzed as the mean ± SD of three independent experiments. *p 

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