HHLA2 Antibody - #DF14396

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製品: HHLA2 Antibody
カタログ: DF14396
タンパク質の説明: Rabbit polyclonal antibody to HHLA2
アプリケーション: IHC IF/ICC
Cited expt.: IF/ICC
反応性: Human
分子量: 47kD(Calculated).
ユニプロット: Q9UM44

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
HHLA2 Antibody detects endogenous levels of total HHLA2.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

B7H7; HERV H LTR associating 2; HERV-H LTR-associating protein 2; HHLA 2; HHLA2; HHLA2_HUMAN; Human endogenous retrovirus H long terminal repeat associating protein 2; Human endogenous retrovirus-H long terminal repeat-associating protein 2;

免疫原

免疫原:

A synthesized peptide derived from Human HHLA2.

Uniprot:

Q9UM44(HHLA2_HUMAN) >>Visit HPA database.

遺伝子(ID):
HHLA2(11148)
発現特異性:
Q9UM44 HHLA2_HUMAN:

Expressed at high levels in colon, kidney, testis, lung and pancreas, and at lower levels in small intestine, liver and skeletal muscle. In immune cells, highly expressed in B-cells, dendritic cells and macrophages. Not detected in T-cells.

タンパク質配列:
MKAQTALSFFLILITSLSGSQGIFPLAFFIYVPMNEQIVIGRLDEDIILPSSFERGSEVVIHWKYQDSYKVHSYYKGSDHLESQDPRYANRTSLFYNEIQNGNASLFFRRVSLLDEGIYTCYVGTAIQVITNKVVLKVGVFLTPVMKYEKRNTNSFLICSVLSVYPRPIITWKMDNTPISENNMEETGSLDSFSINSPLNITGSNSSYECTIENSLLKQTWTGRWTMKDGLHKMQSEHVSLSCQPVNDYFSPNQDFKVTWSRMKSGTFSVLAYYLSSSQNTIINESRFSWNKELINQSDFSMNLMDLNLSDSGEYLCNISSDEYTLLTIHTVHVEPSQETASHNKGLWILVPSAILAAFLLIWSVKCCRAQLEARRSRHPADGAQQERCCVPPGERCPSAPDNGEENVPLSGKV

研究背景

機能:

Through interaction with TMIGD2, costimulates T-cells in the context of TCR-mediated activation. Enhances T-cell proliferation and cytokine production via an AKT-dependent signaling cascade.

細胞の位置付け:

Membrane>Single-pass type I membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at high levels in colon, kidney, testis, lung and pancreas, and at lower levels in small intestine, liver and skeletal muscle. In immune cells, highly expressed in B-cells, dendritic cells and macrophages. Not detected in T-cells.

参考文献

1). Histone lactylation dynamics: Unlocking the triad of metabolism, epigenetics, and immune regulation in metastatic cascade of pancreatic cancer. Cancer letters, 2024 (PubMed: 39019144) [IF=9.1]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 9. Increased HLS in metastasized cells presented with enhanced immune evasion (A–B) Representative multi-color staining of PRM and LMT. Panel1: CK19 (blue), HHLA2 (brown), Pan Kla (grey), NCAM1 (yellow), Galectin-9 (green), and B7H3 (red) (A). Panel2: CK19 (grey), PDL1 (brown), B7H4 (pink), Nectin-2 (yellow), CD3 (green), and CD8 (red) (B) (C–E) Correlation of tumor nuclear score of Pan Kla and mesenchymal proportion of NK, CD8+ T cells and CD8+/CD3+CD8− T cells ratio in PRM and LMT. (F) Circle diagram showing that correlation of IHC score of Pan Kla and other immune checkpoints. (G) Violin diagram showing that difference of mesenchymal and parenchymal score and discrepancy of PRM and LMT. (H) Scatter plot displaying nuclear score of Pan Kla and cytoplastic score of Netin-2 in PRM and LMT. (I) Heatmap showing cytoplastic score of Pan Kla and cytoplastic score of checkpoints and immune infiltration in PRM and LMT. (J) K-M survival analysis of TMA validation cohort. (K) Correlation of immune parameters and Pan Kla score in TMA validation cohort. (L) Distance distribution between Nectin-2+ CK19+ cells and CD8+ T cells in high and low Pan Kla group. (M) Heatmap show the difference in the number and intensity of interactions per cell cluster at PRM and LMT. (N) Comparison of immune-related function in PRM and LMT. MCS, mesenchymal cytoplastic score; MNS, mesenchymal nuclear score; PRM, primary lesions; LMT, liver metastasis; HLS, histone lactylation score; TMA, tissue microarray.

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