FBXL5 Antibody - #DF14329

Figure 4. FBXL5 regulated the IREB2-MFRN2 axis, attenuating mitochondrial iron deposition and protecting AECII cells from single-dose BLM-induced injury and MLE-12 cells from BLM damage. (A-B) IREB2 protein levels in the lungs of mice was detected using western blotting (n = 3). (A and C) IREB2 protein levels in MLE-12 cells was detected using western blotting (n = 3). (D) IREB2 localization in AECII of the control and pulmonary fibrosis mice was determined using anti-IREB2 antibodies (green) and anti-SPC antibodies (red) (Scale bar = 50 μm). (E) IREB2 protein levels in MLE-12 cells was detected using immunofluorescence staining (Scale bar = 100 μm). (F) The interaction between IREB2 and MFRN2 was assessed by dual luciferase reporter gene assay (n = 3). (G-J) FBXL5, IREB2, and MFRN2 protein levels in MLE-12 cells was detected using western blotting (n = 3). (K) MLE-12 cells were stained with Mito-Tracker (red), and iron was stained with Mito-Ferro Green (green), and then they were imaged using confocal microscopy with Airyscan. Representative images are shown (Scale bar = 5 μm), and boxes mark the enlarged images shown (Scale bar = 1 μm). (L) TFAM protein levels in MLE-12 cells was detected using immunofluorescence staining (Scale bar = 100 μm). (M) MLE-12 cells were transduced with Mito-Tracker (red), and the DNA was transduced with anti-DNA antibody (green) and then imaged by confocal microscopy with Airyscan. Representative images are shown (Scale bar = 1 μm), and boxes mark the enlarged images shown (Scale bar = 1 μm). (N) The Δψm in MLE-12 cells was measured using JC-1 staining (Scale bar = 100 μm). * P < 0.05, ** P < 0.01.