MARCKS Antibody - #AF6250

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製品: MARCKS Antibody
カタログ: AF6250
タンパク質の説明: Rabbit polyclonal antibody to MARCKS
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Rabbit, Chicken, Xenopus
分子量: 36,80 kDa; 32kD(Calculated).
ユニプロット: P29966
RRID: AB_2835114

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MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Rabbit(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
MARCKS Antibody detects endogenous levels of total MARCKS.
RRID:
AB_2835114
引用形式: Affinity Biosciences Cat# AF6250, RRID:AB_2835114.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

80 kDa protein; 80K L; 80K L protein; 80K-L protein; 80KL; 81 kDa protein, light chain; light chain; MACS; MARCKS; MARCS; MARCS_HUMAN; MGC52672; myristoylated alanine rich C kinase substrate; Myristoylated alanine rich protein kinase C substrate (MARCKS, 80K L); Myristoylated alanine rich protein kinase C substrate; Myristoylated alanine-rich C-kinase substrate; Phosphomyristin; PKCSL; PRKCSL; protein kinase C substrate 80 kDa protein light chain; Protein kinase C substrate;

免疫原

免疫原:

A synthesized peptide derived from human MARCKS, corresponding to a region within the internal amino acids.

Uniprot:

P29966(MARCS_HUMAN) >>Visit HPA database.

遺伝子(ID):
MARCKS(4082)
タンパク質の説明:
MARCKS the most prominent cellular substrate of protein kinase C. Binds calmodulin, actin, and synapsin. A filamentous actin cross-linking protein. Critical for normal brain development.
タンパク質配列:
MGAQFSKTAAKGEAAAERPGEAAVASSPSKANGQENGHVKVNGDASPAAAESGAKEELQANGSAPAADKEEPAAAGSGAASPSAAEKGEPAAAAAPEAGASPVEKEAPAEGEAAEPGSPTAAEGEAASAASSTSSPKAEDGATPSPSNETPKKKKKRFSFKKSFKLSGFSFKKNKKEAGEGGEAEAPAAEGGKDEAAGGAAAAAAEAGAASGEQAAAPGEEAAAGEEGAAGGDPQEAKPQEAAVAPEKPPASDETKAAEEPSKVEEKKAEEAGASAAACEAPSAAGPGAPPEQEAAPAEEPAAAAASSACAAPSQEAQPECSPEAPPAEAAE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Xenopus
100
Chicken
100
Rabbit
100
Horse
0
Sheep
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

MARCKS is the most prominent cellular substrate for protein kinase C. This protein binds calmodulin, actin, and synapsin. MARCKS is a filamentous (F) actin cross-linking protein.

PTMs:

Phosphorylation by PKC displaces MARCKS from the membrane. It also inhibits the F-actin cross-linking activity.

細胞の位置付け:

Cytoplasm>Cytoskeleton. Membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the MARCKS family.

研究領域

· Human Diseases > Cancers: Overview > MicroRNAs in cancer.

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

参考文献

1). HRH1-targeting mAb: a precision therapeutic strategy for Glioblastoma. Oncogene, 2025 (PubMed: 41125791) [IF=6.9]
2). LINC00525 drives aggressive phenotypes in bladder cancer via YAP stabilization-mediated transcriptional activation. Cancer cell international, 2025 (PubMed: 40528165) [IF=5.8]

Application: WB    Species: Mouse    Sample:

Fig. 6 LINC00525 regulates bladder cancer tumor growth through YAP. A and B. The volume and growth of tumors in the following groups (sh Control, sh-00525, sh-00525 + GA-017). C. Immunohistochemical staining shows the expression of KI67, YAP and pYAPS127 in mice tumors among sh Control, sh-00525, sh-00525 + GA-017 groups. Scale bar: 100 μm. D. Relative protein expression of YAP, pYAPS127, downstream targets genes (such as CYR61, CTGF and ANKRD1) and LINC00525 in mice tumors among sh Control, sh-00525, sh-00525 + GA-017 groups. E-H. The volume and growth of tumors in the following groups (Control, LINC00525, LINC00525 + Cytochalasin D) and analyzed as in C and D

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