RSPH4A Antibody - #DF13889

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製品: RSPH4A Antibody
カタログ: DF13889
タンパク質の説明: Rabbit polyclonal antibody to RSPH4A
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Rat
分子量: 81kD; 81kD(Calculated).
ユニプロット: Q5TD94

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
クローナリティ:
Polyclonal
特異性:
RSPH4A Antibody detects endogenous levels of total RSPH4A.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

A230081C05; CILD11; dJ412I7.1; Radial spoke head protein 4 homolog A; Radial spoke head-like protein 3; RSH4A_HUMAN; RSHL3; Rsph4a; RSPH6B;

免疫原

免疫原:

A synthesized peptide derived from Human RSPH4A.

Uniprot:

Q5TD94(RSH4A_HUMAN) >>Visit HPA database.

遺伝子(ID):
RSPH4A(345895)
発現特異性:
Q5TD94 RSH4A_HUMAN:

Expressed in trachea, lungs, and testes. Very strong expression is detected in nasal brushings.

タンパク質配列:
MEDSTSPKQEKENQEELGETRRPWEGKTAASPQYSEPESSEPLEAKQGPETGRQSRSSRPWSPQSRAKTPLGGPAGPETSSPAPVSPREPSSSPSPLAPARQDLAAPPQSDRTTSVIPEAGTPYPDPLEQSSDKRESTPHHTSQSEGNTFQQSQQPKPHLCGRRDVSYNNAKQKELRFDVFQEEDSNSDYDLQQPAPGGSEVAPSMLEITIQNAKAYLLKTSSNSGFNLYDHLSNMLTKILNERPENAVDIFENISQDVKMAHFSKKFDALQNENELLPTYEIAEKQKALFLQGHLEGVDQELEDEIAENALPNVMESAFYFEQAGVGLGTDETYRIFLALKQLTDTHPIQRCRFWGKILGLEMNYIVAEVEFREGEDEEEVEEEDVAEERDNGESEAHEDEEDELPKSFYKAPQAIPKEESRTGANKYVYFVCNEPGRPWVKLPPVIPAQIVIARKIKKFFTGRLDAPIISYPPFPGNESNYLRAQIARISAGTHVSPLGFYQFGEEEGEEEEEAEGGRNSFEENPDFEGIQVIDLVESLSNWVHHVQHILSQGRCNWFNSIQKNEEEEEEEDEEKDDSDYIEQEVGLPLLTPISEDLEIQNIPPWTTRLSSNLIPQYAIAVLQSNLWPGAYAFSNGKKFENFYIGWGHKYSPDNYTPPVPPPVYQEYPSGPEITEMDDPSVEEEQAFRAAQEAVLLAAENEESEEDEDEEDDYD

研究背景

機能:

Probable component of the axonemal radial spoke head. Radial spokes are regularly spaced along cilia, sperm and flagella axonemes. They consist of a thin stalk which is attached to a subfiber of the outer doublet microtubule, and a bulbous head which is attached to the stalk and appears to interact with the projections from the central pair of microtubules.

細胞の位置付け:

Cytoplasm>Cytoskeleton>Cilium axoneme. Cell projection>Cilium.
Note: Radial spoke.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in trachea, lungs, and testes. Very strong expression is detected in nasal brushings.

タンパク質ファミリー:

Belongs to the flagellar radial spoke RSP4/6 family.

参考文献

1). Association of novel DNAH11 variants with asthenoteratozoospermia lead to male infertility. Human genomics, 2024 (PubMed: 39256880) [IF=3.8]

Application: WB    Species: human    Sample:

Fig. 5 The distribution and expression levels of flagella-associated proteins in patients. (A-D). Immunofluorescence staining assays were performed on the sperm of mutant patients and normal subjects using anti-DNAI2 (red), anti-SPAG6 (red), anti-RSPH4A (red), and anti-TOMM20 (red). The results showed that TOMM20 was located at the mitochondrial sheath, and RSPH4A, DNAI2, and SPAG6 were distributed in full-length flagella in fertile control individual sperm. By contrast, it was found that almost absence of DNAI2, SPAG6, and TOMM20 in sperm from AY0524 and AY0540, RSPH4A was accumulated on the sperm neck, while it was not evidently changed in DNAH9-deficient sperm. Anti-ac-tubulin (green) marked the sperm flagella, and Hoechst (blue) marked the nucleus of spermatozoa. Scale bars: 10 μm. (E) The levels of DNAI2, SPAG6, RSPH4A, and TOMM20 in sperm of patients (AY0524, AY0540, XFY03, and XFY04) reduced markedly compared with control individuals by western blotting analysis. The results of WB assays were in accordance with those of immunofluorescence assays described above. β-actin was used as an internal reference

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