EDD Antibody - #AF0268

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製品: EDD Antibody
カタログ: AF0268
タンパク質の説明: Rabbit polyclonal antibody to EDD
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse
予測: Zebrafish, Horse, Sheep, Rabbit, Xenopus
分子量: 309kDa; 309kD(Calculated).
ユニプロット: O95071
RRID: AB_2833442

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:3000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Zebrafish(100%), Horse(100%), Sheep(88%), Rabbit(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
EDD Antibody detects endogenous levels of total EDD.
RRID:
AB_2833442
引用形式: Affinity Biosciences Cat# AF0268, RRID:AB_2833442.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

4432411E13Rik; AW549941; C77315; D030042K14; DD5; E3 identified by differential display; E3 ubiquitin protein ligase, HECT domain containing, 1; E3 ubiquitin-protein ligase; E3 ubiquitin-protein ligase UBR5; EDD 1; EDD; EDD1; FLJ11310; HECT domain-containing 1; hHYD; HYD; Hyperplastic discs protein homolog; Hyperplastic discs, Drosophila, homolog of; KIAA0896; MGC57263; mKIAA0896; Progestin induced protein; Progestin-induced protein; Rat100; Ubiquitin protein ligase; Ubiquitin protein ligase E3 component n recognin 5; UBR5; UBR5_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human EDD, corresponding to a region within N-terminal amino acids.

Uniprot:

O95071(UBR5_HUMAN) >>Visit HPA database.

遺伝子(ID):
UBR5(51366)
発現特異性:
O95071 UBR5_HUMAN:

Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.

タンパク質の説明:
UBR5 E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific amino-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation.
タンパク質配列:
MTSIHFVVHPLPGTEDQLNDRLREVSEKLNKYNLNSHPPLNVLEQATIKQCVVGPNHAAFLLEDGRVCRIGFSVQPDRLELGKPDNNDGSKLNSNSGAGRTSRPGRTSDSPWFLSGSETLGRLAGNTLGSRWSSGVGGSGGGSSGRSSAGARDSRRQTRVIRTGRDRGSGLLGSQPQPVIPASVIPEELISQAQVVLQGKSRSVIIRELQRTNLDVNLAVNNLLSRDDEDGDDGDDTASESYLPGEDLMSLLDADIHSAHPSVIIDADAMFSEDISYFGYPSFRRSSLSRLGSSRVLLLPLERDSELLRERESVLRLRERRWLDGASFDNERGSTSKEGEPNLDKKNTPVQSPVSLGEDLQWWPDKDGTKFICIGALYSELLAVSSKGELYQWKWSESEPYRNAQNPSLHHPRATFLGLTNEKIVLLSANSIRATVATENNKVATWVDETLSSVASKLEHTAQTYSELQGERIVSLHCCALYTCAQLENSLYWWGVVPFSQRKKMLEKARAKNKKPKSSAGISSMPNITVGTQVCLRNNPLYHAGAVAFSISAGIPKVGVLMESVWNMNDSCRFQLRSPESLKNMEKASKTTEAKPESKQEPVKTEMGPPPSPASTCSDASSIASSASMPYKRRRSTPAPKEEEKVNEEQWSLREVVFVEDVKNVPVGKVLKVDGAYVAVKFPGTSSNTNCQNSSGPDADPSSLLQDCRLLRIDELQVVKTGGTPKVPDCFQRTPKKLCIPEKTEILAVNVDSKGVHAVLKTGNWVRYCIFDLATGKAEQENNFPTSSIAFLGQNERNVAIFTAGQESPIILRDGNGTIYPMAKDCMGGIRDPDWLDLPPISSLGMGVHSLINLPANSTIKKKAAVIIMAVEKQTLMQHILRCDYEACRQYLMNLEQAVVLEQNLQMLQTFISHRCDGNRNILHACVSVCFPTSNKETKEEEEAERSERNTFAERLSAVEAIANAISVVSSNGPGNRAGSSSSRSLRLREMMRRSLRAAGLGRHEAGASSSDHQDPVSPPIAPPSWVPDPPAMDPDGDIDFILAPAVGSLTTAATGTGQGPSTSTIPGPSTEPSVVESKDRKANAHFILKLLCDSVVLQPYLRELLSAKDARGMTPFMSAVSGRAYPAAITILETAQKIAKAEISSSEKEEDVFMGMVCPSGTNPDDSPLYVLCCNDTCSFTWTGAEHINQDIFECRTCGLLESLCCCTECARVCHKGHDCKLKRTSPTAYCDCWEKCKCKTLIAGQKSARLDLLYRLLTATNLVTLPNSRGEHLLLFLVQTVARQTVEHCQYRPPRIREDRNRKTASPEDSDMPDHDLEPPRFAQLALERVLQDWNALKSMIMFGSQENKDPLSASSRIGHLLPEEQVYLNQQSGTIRLDCFTHCLIVKCTADILLLDTLLGTLVKELQNKYTPGRREEAIAVTMRFLRSVARVFVILSVEMASSKKKNNFIPQPIGKCKRVFQALLPYAVEELCNVAESLIVPVRMGIARPTAPFTLASTSIDAMQGSEELFSVEPLPPRPSSDQSSSSSQSQSSYIIRNPQQRRISQSQPVRGRDEEQDDIVSADVEEVEVVEGVAGEEDHHDEQEEHGEENAEAEGQHDEHDEDGSDMELDLLAAAETESDSESNHSNQDNASGRRSVVTAATAGSEAGASSVPAFFSEDDSQSNDSSDSDSSSSQSDDIEQETFMLDEPLERTTNSSHANGAAQAPRSMQWAVRNTQHQRAASTAPSSTSTPAASSAGLIYIDPSNLRRSGTISTSAAAAAAALEASNASSYLTSASSLARAYSIVIRQISDLMGLIPKYNHLVYSQIPAAVKLTYQDAVNLQNYVEEKLIPTWNWMVSIMDSTEAQLRYGSALASAGDPGHPNHPLHASQNSARRERMTAREEASLRTLEGRRRATLLSARQGMMSARGDFLNYALSLMRSHNDEHSDVLPVLDVCSLKHVAYVFQALIYWIKAMNQQTTLDTPQLERKRTRELLELGIDNEDSEHENDDDTNQSATLNDKDDDSLPAETGQNHPFFRRSDSMTFLGCIPPNPFEVPLAEAIPLADQPHLLQPNARKEDLFGRPSQGLYSSSASSGKCLMEVTVDRNCLEVLPTKMSYAANLKNVMNMQNRQKKEGEEQPVLPEETESSKPGPSAHDLAAQLKSSLLAEIGLTESEGPPLTSFRPQCSFMGMVISHDMLLGRWRLSLELFGRVFMEDVGAEPGSILTELGGFEVKESKFRREMEKLRNQQSRDLSLEVDRDRDLLIQQTMRQLNNHFGRRCATTPMAVHRVKVTFKDEPGEGSGVARSFYTAIAQAFLSNEKLPNLECIQNANKGTHTSLMQRLRNRGERDREREREREMRRSSGLRAGSRRDRDRDFRRQLSIDTRPFRPASEGNPSDDPEPLPAHRQALGERLYPRVQAMQPAFASKITGMLLELSPAQLLLLLASEDSLRARVDEAMELIIAHGRENGADSILDLGLVDSSEKVQQENRKRHGSSRSVVDMDLDDTDDGDDNAPLFYQPGKRGFYTPRPGKNTEARLNCFRNIGRILGLCLLQNELCPITLNRHVIKVLLGRKVNWHDFAFFDPVMYESLRQLILASQSSDADAVFSAMDLAFAIDLCKEEGGGQVELIPNGVNIPVTPQNVYEYVRKYAEHRMLVVAEQPLHAMRKGLLDVLPKNSLEDLTAEDFRLLVNGCGEVNVQMLISFTSFNDESGENAEKLLQFKRWFWSIVEKMSMTERQDLVYFWTSSPSLPASEEGFQPMPSITIRPPDDQHLPTANTCISRLYVPLYSSKQILKQKLLLAIKTKNFGFV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Xenopus
100
Zebrafish
100
Rabbit
100
Sheep
88
Pig
0
Bovine
0
Dog
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

E3 ubiquitin-protein ligase which is a component of the N-end rule pathway. Recognizes and binds to proteins bearing specific N-terminal residues that are destabilizing according to the N-end rule, leading to their ubiquitination and subsequent degradation (By similarity). Involved in maturation and/or transcriptional regulation of mRNA by activating CDK9 by polyubiquitination. May play a role in control of cell cycle progression. May have tumor suppressor function. Regulates DNA topoisomerase II binding protein (TopBP1) in the DNA damage response. Plays an essential role in extraembryonic development. Ubiquitinates acetylated PCK1. Also acts as a regulator of DNA damage response by acting as a suppressor of RNF168, an E3 ubiquitin-protein ligase that promotes accumulation of 'Lys-63'-linked histone H2A and H2AX at DNA damage sites, thereby acting as a guard against excessive spreading of ubiquitinated chromatin at damaged chromosomes.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed. Most abundant in testis and expressed at high levels in brain, pituitary and kidney.

研究領域

· Genetic Information Processing > Folding, sorting and degradation > Ubiquitin mediated proteolysis.   (View pathway)

参考文献

1). Targeting Viperin prevents coxsackievirus B3-induced acute heart failure. Cell discovery, 2025 (PubMed: 40195316) [IF=33.5]

Application: WB    Species: Mouse    Sample: heart tissues

Fig. 4: Viperin promotes STAT1 ubiquitination and degradation dependently on UBR5. a Western blot analysis of STAT1 levels in HEK293T cells transfected with Flag-Viperin and treated with MG132 (10 μM) as indicated. b Immunoprecipitation analysis of STAT1 ubiquitination in HL-1 cells transfected with or without Myc-Viperin and then treated with MG132 (10 μM, 4 h). c cycloheximide (CHX) pulse chase assay was used to analyze Flag-STAT1 protein stability in HEK293T cells co-transfected with Flag-STAT1 and HA-Viperin, followed by CHX treatment (50 μM) for 12 h and 36 h. d Mass spectrometry analysis of Viperin-binding proteins in HEK293T cells transfected with Flag-Viperin. The putative ubiquitin E3 ligases interacting with Viperin are listed. Western blot was used to analyze Myc-STAT1 levels in cells with knockout of these ubiquitin E3 ligases that were transfected with or without HA-Viperin. e Immunoprecipitation analysis of the interaction between UBR5 and STAT1 in HL-1 cells transfected with or without HA-Viperin. f, g Ubr5+/+ or Ubr5−/− cells were made by CRISPR-Cas9 in both stable Viperin-expressing HEK293T cells (f) and intact HEK293T cells (Viperin protein-deficient) (g). Immunoprecipitation was used to analyze STAT1 ubiquitination. h In vitro ubiquitination assay for analysis of Flag-STAT1 ubiquitination by the recombinant UBR5 proteins. Flag-STAT1 was immunoprecipitated by Flag beads. Flag-STAT1 ubiquitination was determined using an anti-Flag antibody using western blot analysis. i Immunoprecipitation analysis of STAT1 ubiquitination types in stable Viperin-expressing HEK293T cells cotransfected with Myc-STAT1 and different types of HA-Ub. j Western blot analysis of STAT1 levels in Ubr5+/+ or Ubr5−/− cells transfected with HA-Viperin. k Western blot analysis of STAT1, SGK1 and cytomembrane KCNQ1 levels in Ubr5+/+ or Ubr5−/− HL-1 cells infected with or without CVB3 (MOI = 10) for 12 h. l RT-qPCR analysis of Sgk1 mRNA in Ubr5+/+ or Ubr5−/− HL-1 cells infected with or without CVB3 (MOI = 10) for 12 h. NS, not significant (P > 0.05), ***P 

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