Interleukin 34 Antibody - #DF13820

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製品: Interleukin 34 Antibody
カタログ: DF13820
タンパク質の説明: Rabbit polyclonal antibody to Interleukin 34
アプリケーション: IHC IF/ICC
Cited expt.: IHC
反応性: Human
分子量: 40~50kDa(Observed); 27kD(Calculated).
ユニプロット: Q6ZMJ4

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Interleukin 34 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
クローナリティ:
Polyclonal
特異性:
Interleukin 34 Antibody detects endogenous levels of total Interleukin 34.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

C16orf77; Chromosome 16 open reading frame 77; FPT025; IL 34; IL-34; Il34; IL34_HUMAN; Interleukin 34; Interleukin-34; Interleukin34; MGC34647;

免疫原

免疫原:

A synthesized peptide derived from Human Interleukin 34.

Uniprot:

Q6ZMJ4(IL34_HUMAN) >>Visit HPA database.

遺伝子(ID):
IL34(146433)
発現特異性:
Q6ZMJ4 IL34_HUMAN:

Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.

タンパク質配列:
MPRGFTWLRYLGIFLGVALGNEPLEMWPLTQNEECTVTGFLRDKLQYRSRLQYMKHYFPINYKISVPYEGVFRIANVTRLQRAQVSERELRYLWVLVSLSATESVQDVLLEGHPSWKYLQEVETLLLNVQQGLTDVEVSPKVESVLSLLNAPGPNLKLVRPKALLDNCFRVMELLYCSCCKQSSVLNWQDCEVPSPQSCSPEPSLQYAATQLYPPPPWSPSSPPHSTGSVRPVRAQGEGLLP

研究背景

機能:

Cytokine that promotes the proliferation, survival and differentiation of monocytes and macrophages. Promotes the release of proinflammatory chemokines, and thereby plays an important role in innate immunity and in inflammatory processes. Plays an important role in the regulation of osteoclast proliferation and differentiation, and in the regulation of bone resorption. Signaling via CSF1R and its downstream effectors stimulates phosphorylation of MAPK1/ERK2 AND MAPK3/ERK1.

細胞の位置付け:

Secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in the sinusoidal epithelium in the red pulp of spleen (at protein level). Predominantly expressed in spleen. Also detected in a range of other tissues including heart, brain, lung, liver, kidney, thymus, testis, ovary, small intestine, prostate and colon.

タンパク質ファミリー:

Belongs to the IL-34 family.

参考文献

1). Interactions between MFAP5 + fibroblasts and tumor-infiltrating myeloid cells shape the malignant microenvironment of colorectal cancer. Journal of translational medicine, 2023 (PubMed: 37344903) [IF=7.4]

Application: IHC    Species: human    Sample:

Fig. 6 MFAP5 + fibroblasts modulate the phenotype of C1QC + macrophages through the IL34/CSF1R signal pathway. A The hierarchy plots of CSF signaling pathway network indicate the regulation of MFAP5 + fibroblasts and FABP5 + fibroblasts on myeloid cells in normal or tumor sites. B Violin plot of IL34, CSF1, and CSF1R expression of tumor-specific fibroblasts and myeloid cells in distinct tissues (normal or tumor). C Heatmap shows dominant senders, receivers, mediators and influencers in CSF signals of tumor inferred by network centrality score. D Bar plot shows the contribution of all L-R pairs in CSF pathway within the normal tissue. E Spatial images localize the expression of IL34 and CSF1R which suggest that the co-localization of IL34 and CSF1R in CRC slices. F Heat map of M1/M2 phenotype scores of three identified tumor-associated macrophage subtypes. G Immunohistochemical double-staining of MFAP5/CD163 and IL-34/CSF1R using continuous slides of CRC tissues (×20, 50 μm)

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