MYOCD Antibody - #AF0023

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製品: MYOCD Antibody
カタログ: AF0023
タンパク質の説明: Rabbit polyclonal antibody to MYOCD
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Rabbit, Dog, Chicken, Xenopus
分子量: 100 kDa; 102kD(Calculated).
ユニプロット: Q8IZQ8

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IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
MYOCD Antibody detects endogenous levels of total MYOCD.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline, pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

MYCD; MYCD_HUMAN; Myocardin; Myocd;

免疫原

免疫原:

A synthesized peptide derived from human MYOCD, corresponding to a region within the internal amino acids.

Uniprot:

Q8IZQ8(MYCD_HUMAN) >>Visit HPA database.

遺伝子(ID):
MYOCD(93649)
発現特異性:
Q8IZQ8 MYCD_HUMAN:

Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.

タンパク質の説明:
Transcriptional factor that uses the canonical single or multiple CArG boxes DNA sequence. Binds CArG boxes only in the presence of serum response factor (SRF). Acts as a cofactor of SRF and modulates SRF-target genes. Regulates the expression of a set of cardiac and smooth muscle-specific genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage.
タンパク質配列:
MTLLGSEHSLLIRSKFRSVLQLRLQQRRTQEQLANQGIIPPLKRPAEFHEQRKHLDSDKAKNSLKRKARNRCNSADLVNMHILQASTAERSIPTAQMKLKRARLADDLNEKIALRPGPLELVEKNILPVDSAVKEAIKGNQVSFSKSTDAFAFEEDSSSDGLSPDQTRSEDPQNSAGSPPDAKASDTPSTGSLGTNQDLASGSENDRNDSASQPSHQSDAGKQGLGPPSTPIAVHAAVKSKSLGDSKNRHKKPKDPKPKVKKLKYHQYIPPDQKAEKSPPPMDSAYARLLQQQQLFLQLQILSQQQQQQQHRFSYLGMHQAQLKEPNEQMVRNPNSSSTPLSNTPLSPVKNSFSGQTGVSSFKPGPLPPNLDDLKVSELRQQLRIRGLPVSGTKTALMDRLRPFQDCSGNPVPNFGDITTVTFPVTPNTLPNYQSSSSTSALSNGFYHFGSTSSSPPISPASSDLSVAGSLPDTFNDASPSFGLHPSPVHVCTEESLMSSLNGGSVPSELDGLDSEKDKMLVEKQKVINELTWKLQQEQRQVEELRMQLQKQKRNNCSEKKPLPFLAASIKQEEAVSSCPFASQVPVKRQSSSSECHPPACEAAQLQPLGNAHCVESSDQTNVLSSTFLSPQCSPQHSPLGAVKSPQHISLPPSPNNPHFLPSSSGAQGEGHRVSSPISSQVCTAQMAGLHSSDKVGPKFSIPSPTFSKSSSAISEVTQPPSYEDAVKQQMTRSQQMDELLDVLIESGEMPADAREDHSCLQKVPKIPRSSRSPTAVLTKPSASFEQASSGSQIPFDPYATDSDEHLEVLLNSQSPLGKMSDVTLLKIGSEEPHFDGIMDGFSGKAAEDLFNAHEILPGPLSPMQTQFSPSSVDSNGLQLSFTESPWETMEWLDLTPPNSTPGFSALTTSSPSIFNIDFLDVTDLNLNSSMDLHLQQW

PTMs - Q8IZQ8 基板として

Site PTM Type Enzyme
S74 Phosphorylation
K138 Sumoylation
S178 Phosphorylation
S229 Phosphorylation
T230 Phosphorylation
S242 Phosphorylation
K264 Methylation
S451 Phosphorylation P49841 (GSK3B)
S455 Phosphorylation P49841 (GSK3B)
S459 Phosphorylation P49841 (GSK3B)
S463 Phosphorylation P49841 (GSK3B)
K571 Sumoylation
K588 Acetylation
S626 Phosphorylation P49841 (GSK3B)
S630 Phosphorylation P49841 (GSK3B)
S634 Phosphorylation P49841 (GSK3B)
S638 Phosphorylation P49841 (GSK3B)
S704 Phosphorylation
T732 Phosphorylation
S734 Phosphorylation
S815 Phosphorylation P28482 (MAPK1)
S862 Phosphorylation P28482 (MAPK1)
S869 Phosphorylation P28482 (MAPK1)
T896 Phosphorylation P28482 (MAPK1)

研究背景

機能:

Smooth muscle cells (SM) and cardiac muscle cells-specific transcriptional factor which uses the canonical single or multiple CArG boxes DNA sequence. Acts as a cofactor of serum response factor (SRF) with the potential to modulate SRF-target genes. Plays a crucial role in cardiogenesis and differentiation of the smooth muscle cell lineage (myogenesis) (By similarity).

PTMs:

Phosphorylation regulates negatively the intrinsic myocardin transcriptional activity.

細胞の位置付け:

Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in heart, aorta, and in smooth muscle cell-containing tissues: stomach, bladder, small intestine, colon, lung, placenta and uterus. Very faint expression in prostate and skeletal muscle.

サブユニット構造:

Homodimer. Interacts with SRF, its association does not depend on specific DNA sequences for ternary complex formation (By similarity). Interacts with MLLT7/FOXO4. Interacts (via C-terminal) with EP300 (via the CREB-binding domain). Interacts with HDAC4 and HDAC5 (By similarity). Interacts with MEF2C (By similarity).

タンパク質ファミリー:

The C-terminal region contains a general transcription activation domain. The N-terminal region, comprising a basic and a Gln-rich domain, confers transcriptional potency and specificity by mediating association with the MADS box of SRF. The basic domain may be required for nuclear localization. The SAP domain is important for transactivation and ternary complex formation (By similarity).

参考文献

1). MBNL1 regulates isoproterenol‐induced myocardial remodelling in vitro and in vivo. JOURNAL OF CELLULAR AND MOLECULAR MEDICINE (PubMed: 33295096) [IF=5.3]

Application: WB    Species: mice    Sample: cardiomyocytes

FIGURE 3 MBNL1 increases Myocardin expression by binding to UGCU at the 3'-UTR of Myocardin mRNA. A, The 3'-UTR region of Myocardin contains the binding site of MBNL1. B, The binding of MBNL1 to Myocardin mRNA was validated using RIP. (n = 3, *, P < .05). C-F, The changes in Myocardin and MBNL1 in cardiomyocytes with overexpressed or silenced MBNL1 were measured using realtime PCR and Western blotting. (n = 3, *, P < .05, **, P < .01). G and H, Myocardin mRNA residues after 0, 20, 40 and 60 min in cardiomyocytes treated with Actinomycin D (ACD, 5 μg/mL) and with overexpressed or silenced MBNL1were measured using realtime PCR. I, The 3'-UTR region of Myocardin mRNA was transcribed in vitro and labelled with biotin. The combination of MBNL1 protein and 3'-UTR of Myocardin was detected using RNA pulldown assay. J, The RNA probes corresponding to nine prediction sites (probe # 10-18) and their corresponding antisense probes (probe # 1-9) were synthesized and biotin-labelled. The combination site of MBNL1 protein in the 3'-UTR of Myocardin was confirmed using RNA pull-down assay
2). LncRNA-Mhrt regulates cardiac hypertrophy by modulating the miR-145a-5p/KLF4/myocardin axis. JOURNAL OF MOLECULAR AND CELLULAR CARDIOLOGY (PubMed: 31982428) [IF=5.0]

Application: WB    Species: mouse    Sample: primary cardiomyocytes

Fig. 2. |Mhrt regulates myocardin expression through KLF4 in primary cardiomyocytes.(D, E and F) Following knock down of endogenous KLF4 in primary cardiomyocytes, the effect of Mhrt on myocardin mRNA and protein levels was examined (n = 3, *,P < .05, **, P < .01, #, P > .05).
3). Myocardin Reverses Hypoxia-Inducible Factor-1α Mediated Phenotypic Modulation of Corpus Cavernosum Smooth Muscle Cells in Hypoxia Induced by. World Journal of Mens Health (PubMed: 35274501) [IF=4.8]
4). KLHL38 facilitates STS‐induced apoptosis in HL‐1 cells via myocardin degradation. IUBMB LIFE (PubMed: 35112472) [IF=4.6]

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