Thrombin Receptor Antibody - #AF0263

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製品: Thrombin Receptor Antibody
カタログ: AF0263
タンパク質の説明: Rabbit polyclonal antibody to Thrombin Receptor
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 48kDa; 47kD(Calculated).
ユニプロット: P25116
RRID: AB_2833437

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:3000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Thrombin Receptor Antibody detects endogenous levels of total Thrombin Receptor.
RRID:
AB_2833437
引用形式: Affinity Biosciences Cat# AF0263, RRID:AB_2833437.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CF2R; Coagulation factor II (thrombin) receptor; Coagulation factor II receptor; F2R; HTR; PAR 1; PAR-1; PAR1; PAR1_HUMAN; Protease activated receptor 1; Proteinase activated receptor 1; Proteinase-activated receptor 1; Thrombin receptor; TR;

免疫原

免疫原:
Uniprot:

P25116(PAR1_HUMAN) >>Visit HPA database.

遺伝子(ID):
F2R(2149)
発現特異性:
P25116 PAR1_HUMAN:

Platelets and vascular endothelial cells.

タンパク質の説明:
PAR1 a G-protein coupled high-affinity receptor for activated thrombin or trypsin. Coupled to G proteins that stimulate phosphoinositide hydrolysis. Coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelet activation and in vascular development.
タンパク質配列:
MGPRRLLLVAACFSLCGPLLSARTRARRPESKATNATLDPRSFLLRNPNDKYEPFWEDEEKNESGLTEYRLVSINKSSPLQKQLPAFISEDASGYLTSSWLTLFVPSVYTGVFVVSLPLNIMAIVVFILKMKVKKPAVVYMLHLATADVLFVSVLPFKISYYFSGSDWQFGSELCRFVTAAFYCNMYASILLMTVISIDRFLAVVYPMQSLSWRTLGRASFTCLAIWALAIAGVVPLLLKEQTIQVPGLNITTCHDVLNETLLEGYYAYYFSAFSAVFFFVPLIISTVCYVSIIRCLSSSAVANRSKKSRALFLSAAVFCIFIICFGPTNVLLIAHYSFLSHTSTTEAAYFAYLLCVCVSSISCCIDPLIYYYASSECQRYVYSILCCKESSDPSSYNSSGQLMASKMDTCSSNLNNSIYKKLLT

PTMs - P25116 基板として

Site PTM Type Enzyme
K51 Ubiquitination
Y52 Phosphorylation
Y69 Phosphorylation
S306 Phosphorylation
S375 Phosphorylation
S376 Phosphorylation
Y383 Phosphorylation
S384 Phosphorylation
S391 Phosphorylation
S392 Phosphorylation
S395 Phosphorylation
S396 Phosphorylation
Y397 Phosphorylation
S399 Phosphorylation
S400 Phosphorylation
S406 Phosphorylation
K407 Ubiquitination
T410 Phosphorylation
S412 Phosphorylation
S413 Phosphorylation
S418 Phosphorylation
Y420 Phosphorylation
K421 Ubiquitination
K422 Ubiquitination
T425 Phosphorylation

研究背景

機能:

High affinity receptor for activated thrombin coupled to G proteins that stimulate phosphoinositide hydrolysis. May play a role in platelets activation and in vascular development.

PTMs:

A proteolytic cleavage generates a new N-terminus that functions as a tethered ligand.

Phosphorylated in the C-terminal tail; probably mediating desensitization prior to the uncoupling and internalization of the receptor.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Platelets and vascular endothelial cells.

タンパク質ファミリー:

The cleaved signal peptide may not be degraded and may function as an intracellular angiogenesis inhibitor peptide known as parstatin.

Belongs to the G-protein coupled receptor 1 family.

研究領域

· Cellular Processes > Cell motility > Regulation of actin cytoskeleton.   (View pathway)

· Environmental Information Processing > Signal transduction > Rap1 signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Environmental Information Processing > Signal transduction > PI3K-Akt signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Organismal Systems > Immune system > Complement and coagulation cascades.   (View pathway)

· Organismal Systems > Immune system > Platelet activation.   (View pathway)

参考文献

1). Inhibiting trophoblast PAR-1 overexpression suppresses sFlt-1-induced anti-angiogenesis and abnormal vascular remodeling: a possible therapeutic approach for preeclampsia. MOLECULAR HUMAN REPRODUCTION (PubMed: 29325127) [IF=4.0]
2). pHLIP(Var7)-P1AP suppresses tumor cell proliferation in MDA-MB-231 triple-negative breast cancer by targeting protease activated receptor 1. BREAST CANCER RESEARCH AND TREATMENT (PubMed: 32034579) [IF=3.8]

Application: IF/ICC    Species: Human    Sample: MDA-MB-231 and MCF-10A cells

Fig. 1 PAR1 expression was positive in MDA-MB-231 cells, and PAR1 expression was negative in MCF-10A cells (400 ×)
3). Doxycycline directly targets PAR1 to suppress tumor progression. Oncotarget (PubMed: 28187433)

Application: IF/ICC    Species: human    Sample:

(G) Confocal immunofluorescence imaging was performed to visualize PAR1 and doxycycline. PAR1 and doxycycline co-localize, with a Pearson’s correlation coeffcient of 0.944 and a Mander’s overlap of 0.964.

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