Phospho-Nucleophosmin (Ser125) Antibody - #AF3740

(2)   (16)  
製品: Phospho-Nucleophosmin (Ser125) Antibody
カタログ: AF3740
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Nucleophosmin (Ser125)
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
分子量: 37kDa; 33kD(Calculated).
ユニプロット: P06748
RRID: AB_2847054

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $350 在庫あり
 200ul $450 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
関連ダウンロード
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-Nucleophosmin (Ser125) Antibody detects endogenous levels of Nucleophosmin only when phosphorylated at Ser125.
RRID:
AB_2847054
引用形式: Affinity Biosciences Cat# AF3740, RRID:AB_2847054.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

B23; MGC104254; NO38; NPM; NPM_HUMAN; NPM1; Nucleolar phosphoprotein B23; Nucleolar protein NO38; Nucleophosmin (nucleolar phosphoprotein B23 numatrin); Nucleophosmin; nucleophosmin nucleoplasmin family member 1; Nucleophosmin/nucleoplasmin family member 1; Numatrin; OTTHUMP00000161024; OTTHUMP00000161025; OTTHUMP00000223397; OTTHUMP00000223398;

免疫原

免疫原:

A synthesized peptide derived from human Nucleophosmin around the phosphorylation site of Ser125.

Uniprot:

P06748(NPM_HUMAN) >>Visit HPA database.

遺伝子(ID):
NPM1(4869)
タンパク質配列:
MEDSMDMDMSPLRPQNYLFGCELKADKDYHFKVDNDENEHQLSLRTVSLGAGAKDELHIVEAEAMNYEGSPIKVTLATLKMSVQPTVSLGGFEITPPVVLRLKCGSGPVHISGQHLVAVEEDAESEDEEEEDVKLLSISGKRSAPGGGSKVPQKKVKLAADEDDDDDDEEDDDEDDDDDDFDDEEAEEKAPVKKSIRDTPAKNAQKSNQNGKDSKPSSTPRSKGQESFKKQEKTPKTPKGPSSVEDIKAKMQASIEKGGSLPKVEAKFINYVKNCFRMTDQEAIQDLWQWRKSL

研究背景

機能:

Involved in diverse cellular processes such as ribosome biogenesis, centrosome duplication, protein chaperoning, histone assembly, cell proliferation, and regulation of tumor suppressors p53/TP53 and ARF. Binds ribosome presumably to drive ribosome nuclear export. Associated with nucleolar ribonucleoprotein structures and bind single-stranded nucleic acids. Acts as a chaperonin for the core histones H3, H2B and H4. Stimulates APEX1 endonuclease activity on apurinic/apyrimidinic (AP) double-stranded DNA but inhibits APEX1 endonuclease activity on AP single-stranded RNA. May exert a control of APEX1 endonuclease activity within nucleoli devoted to repair AP on rDNA and the removal of oxidized rRNA molecules. In concert with BRCA2, regulates centrosome duplication. Regulates centriole duplication: phosphorylation by PLK2 is able to trigger centriole replication. Negatively regulates the activation of EIF2AK2/PKR and suppresses apoptosis through inhibition of EIF2AK2/PKR autophosphorylation. Antagonizes the inhibitory effect of ATF5 on cell proliferation and relieves ATF5-induced G2/M blockade. In complex with MYC enhances the transcription of MYC target genes.

PTMs:

Acetylated at C-terminal lysine residues, thereby increasing affinity to histones.

ADP-ribosylated.

Phosphorylated at Ser-4 by PLK1 and PLK2. Phosphorylation at Ser-4 by PLK2 in S phase is required for centriole duplication and is sufficient to trigger centriole replication. Phosphorylation at Ser-4 by PLK1 takes place during mitosis. Phosphorylated by CDK2 at Ser-125 and Thr-199. Phosphorylation at Thr-199 may trigger initiation of centrosome duplication. Phosphorylated by CDK1 at Thr-199, Thr-219, Thr-234 and Thr-237 during cell mitosis. When these four sites are phosphorated, RNA-binding activity seem to be abolished. May be phosphorylated at Ser-70 by NEK2. The Thr-199 phosphorylated form has higher affinity for ROCK2. CDK6 triggers Thr-199 phosphorylation when complexed to Kaposi's sarcoma herpesvirus (KSHV) V-cyclin, leading to viral reactivation by reducing viral LANA levels.

Sumoylated by ARF.

Ubiquitinated. Ubiquitination leads to proteasomal degradation. Deubiquitinated by USP36.

細胞の位置付け:

Nucleus>Nucleolus. Nucleus>Nucleoplasm. Cytoplasm>Cytoskeleton>Microtubule organizing center>Centrosome.
Note: Generally nucleolar, but is translocated to the nucleoplasm in case of serum starvation or treatment with anticancer drugs. Has been found in the cytoplasm in patients with primary acute myelogenous leukemia (AML), but not with secondary AML. Can shuttle between cytoplasm and nucleus. Co- localizes with the methylated form of RPS10 in the granular component (GC) region of the nucleolus. Colocalized with nucleolin and APEX1 in nucleoli. Isoform 1 of NEK2 is required for its localization to the centrosome during mitosis.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the nucleoplasmin family.

参考文献

1). AURKA inhibition induces Ewing's sarcoma apoptosis and ferroptosis through NPM1/YAP1 axis. Cell death & disease, 2024 (PubMed: 38287009) [IF=8.1]

Application: WB    Species: human    Sample: ES cell

Fig. 5: AURKA promoted ES cell apoptosis and ferroptosis resistance via phosphorylating Thr95 of NPM1. A Silver staining of co-immunoprecipitation with AURKA antibody in A673. B Results of protein mass spectrometry revealed the physical interaction of AURKA and NPM1. C Results of immunoprecipitation to confirm the interaction relationship of AURKA and NPM1 in ES cells. The corresponding original western blots are shown in Fig. S9. D WB analysis identified the expression level changes of p-NPM1 at Ser4, Thr95, Ser125, Thr199, and the total NPM1 in ES cell lines after treatment with TCS7010. The corresponding original western blots are shown in Fig. S10. E WB analysis identified the expression level changes of p-NPM1 at Ser4, Thr95, Ser125, Thr199, and the total NPM1 in ES cell lines after AURKA knockdown. The corresponding original western blots are shown in Fig. S10. F Colony formation assay showed the reproductive ability of ES cells with NPM1 knockdown. G Colony formation assay determined the reproductive ability of ES cells with NPM1 knockdown in the absence or presence of indicated cell death inhibitors (10 µM ZVAD-FMK and 10 µM ferrostatin-1). H Columnar statistical chart based on the CCK-8 assays indicated the cell viability after NPM1 knockdown in the absence or presence of indicated cell death inhibitors (10 µM ZVAD-FMK and 10 µM ferrostatin-1) for 48 h. I Columnar statistical chart indicated changes in the apoptosis rates of ES cell lines after NPM1 inhibition with siRNA. J Detection of the intracellular ROS levels in ES cells after NPM1 inhibition. K Detection of the intracellular lipid ROS levels in ES cells after NPM1 inhibition. L Changes of the relative intracellular Fe2+ levels in ES cells after NPM1 inhibition. M WB analysis indicated changes in the apoptosis-related gene markers (PARP, Bcl2, Bax) and the ferroptosis-related marker GPX4 after NPM1 inhibition in ES cells. The corresponding original western blots are shown in Fig. S11. Values represented the mean ± SD from 3 independent experiments.
2). TOM1L1 mediated the sort of tumor suppressive miR-378a-3p into exosomes and the excretion out of cells to promote ESCC progression. Cancer gene therapy, 2025 (PubMed: 40123000) [IF=4.8]

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.