製品: Phospho-EPRS (Ser886) Antibody
カタログ: AF3737
タンパク質の説明: Rabbit polyclonal antibody to Phospho-EPRS (Ser886)
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
分子量: 171kD(Calculated).
ユニプロット: P07814
RRID: AB_2847051

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製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Phospho-EPRS (Ser886) Antibody detects endogenous levels of EPRS only when phosphorylated at Ser886.
RRID:
AB_2847051
引用形式: Affinity Biosciences Cat# AF3737, RRID:AB_2847051.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Bifunctional aminoacyl tRNA synthetase; Bifunctional aminoacyl-tRNA synthetase; Bifunctional glutamate/proline tRNA ligase; Cell proliferation-inducing gene 32 protein; DKFZp313B047; EARS; Eprs; GLNS; Glu pro tRNA synthetase; GLUPRORS; GluRS; Glutamate tRNA ligase; Glutamatyl prolyl tRNA synthetase; Glutaminyl tRNA synthetase; Glutamyl prolyl tRNA synthetase; Glutamyl tRNA synthetase; Glutamyl-tRNA synthetase; PARS; PIG 32; PIG32; Proliferation inducing gene 32 protein; Proliferation inducing protein 32; Proline tRNA ligase; Proline--tRNA ligase; Prolyl tRNA synthetase; Prolyl-tRNA synthetase; QARS; QPRS; SYEP_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human EPRS around the phosphorylation site of Ser886.

Uniprot:
遺伝子(ID):
タンパク質配列:
MATLSLTVNSGDPPLGALLAVEHVKDDVSISVEEGKENILHVSENVIFTDVNSILRYLARVATTAGLYGSNLMEHTEIDHWLEFSATKLSSCDSFTSTINELNHCLSLRTYLVGNSLSLADLCVWATLKGNAAWQEQLKQKKAPVHVKRWFGFLEAQQAFQSVGTKWDVSTTKARVAPEKKQDVGKFVELPGAEMGKVTVRFPPEASGYLHIGHAKAALLNQHYQVNFKGKLIMRFDDTNPEKEKEDFEKVILEDVAMLHIKPDQFTYTSDHFETIMKYAEKLIQEGKAYVDDTPAEQMKAEREQRIDSKHRKNPIEKNLQMWEEMKKGSQFGQSCCLRAKIDMSSNNGCMRDPTLYRCKIQPHPRTGNKYNVYPTYDFACPIVDSIEGVTHALRTTEYHDRDEQFYWIIEALGIRKPYIWEYSRLNLNNTVLSKRKLTWFVNEGLVDGWDDPRFPTVRGVLRRGMTVEGLKQFIAAQGSSRSVVNMEWDKIWAFNKKVIDPVAPRYVALLKKEVIPVNVPEAQEEMKEVAKHPKNPEVGLKPVWYSPKVFIEGADAETFSEGEMVTFINWGNLNITKIHKNADGKIISLDAKLNLENKDYKKTTKVTWLAETTHALPIPVICVTYEHLITKPVLGKDEDFKQYVNKNSKHEELMLGDPCLKDLKKGDIIQLQRRGFFICDQPYEPVSPYSCKEAPCVLIYIPDGHTKEMPTSGSKEKTKVEATKNETSAPFKERPTPSLNNNCTTSEDSLVLYNRVAVQGDVVRELKAKKAPKEDVDAAVKQLLSLKAEYKEKTGQEYKPGNPPAEIGQNISSNSSASILESKSLYDEVAAQGEVVRKLKAEKSPKAKINEAVECLLSLKAQYKEKTGKEYIPGQPPLSQSSDSSPTRNSEPAGLETPEAKVLFDKVASQGEVVRKLKTEKAPKDQVDIAVQELLQLKAQYKSLIGVEYKPVSATGAEDKDKKKKEKENKSEKQNKPQKQNDGQRKDPSKNQGGGLSSSGAGEGQGPKKQTRLGLEAKKEENLADWYSQVITKSEMIEYHDISGCYILRPWAYAIWEAIKDFFDAEIKKLGVENCYFPMFVSQSALEKEKTHVADFAPEVAWVTRSGKTELAEPIAIRPTSETVMYPAYAKWVQSHRDLPIKLNQWCNVVRWEFKHPQPFLRTREFLWQEGHSAFATMEEAAEEVLQILDLYAQVYEELLAIPVVKGRKTEKEKFAGGDYTTTIEAFISASGRAIQGGTSHHLGQNFSKMFEIVFEDPKIPGEKQFAYQNSWGLTTRTIGVMTMVHGDNMGLVLPPRVACVQVVIIPCGITNALSEEDKEALIAKCNDYRRRLLSVNIRVRADLRDNYSPGWKFNHWELKGVPIRLEVGPRDMKSCQFVAVRRDTGEKLTVAENEAETKLQAILEDIQVTLFTRASEDLKTHMVVANTMEDFQKILDSGKIVQIPFCGEIDCEDWIKKTTARDQDLEPGAPSMGAKSLCIPFKPLCELQPGAKCVCGKNPAKYYTLFGRSY

PTMs - P07814 基板として

Site PTM Type Enzyme
S29 Phosphorylation
T64 Phosphorylation
C92 S-Nitrosylation
C105 S-Nitrosylation
K139 Ubiquitination
K173 Ubiquitination
K186 Ubiquitination
K197 Ubiquitination
K216 Ubiquitination
Y224 Phosphorylation
K229 Methylation
K229 Ubiquitination
K231 Methylation
K243 Acetylation
K245 Acetylation
K282 Acetylation
K282 Ubiquitination
K288 Ubiquitination
K300 Acetylation
K300 Ubiquitination
K318 Sumoylation
K328 Methylation
K328 Ubiquitination
S345 Phosphorylation
S346 Phosphorylation
T355 Phosphorylation
Y357 Phosphorylation
K360 Ubiquitination
K370 Acetylation
K370 Ubiquitination
Y377 Phosphorylation
K417 Acetylation
K417 Ubiquitination
Y423 Phosphorylation
S434 Phosphorylation
K435 Ubiquitination
K437 Ubiquitination
R454 Methylation
R464 Methylation
T467 Phosphorylation
K472 Ubiquitination
K497 Acetylation
K497 Ubiquitination
K498 Acetylation
K498 Ubiquitination
K512 Ubiquitination
K513 Ubiquitination
K528 Acetylation
K535 Acetylation
K535 Ubiquitination
K542 Acetylation
K542 Ubiquitination
Y546 Phosphorylation
S547 Phosphorylation
K581 Ubiquitination
K586 Ubiquitination
K593 Ubiquitination
K599 Acetylation
K599 Ubiquitination
K637 Acetylation
K642 Ubiquitination
K647 Ubiquitination
K666 Ubiquitination
Y684 Phosphorylation
S688 Phosphorylation
Y690 Phosphorylation
S691 Phosphorylation
K708 Ubiquitination
S713 Phosphorylation
K718 Ubiquitination
K720 Ubiquitination
K725 Ubiquitination
K733 Ubiquitination
S739 Phosphorylation
T745 Phosphorylation
T746 Phosphorylation
S747 Phosphorylation
S750 Phosphorylation
Y754 Phosphorylation
K782 Ubiquitination
K788 Acetylation
S813 Phosphorylation
S814 Phosphorylation
S816 Phosphorylation
S817 Phosphorylation
S819 Phosphorylation
S825 Phosphorylation
Y827 Phosphorylation
S845 Phosphorylation
K849 Ubiquitination
C856 S-Nitrosylation
S859 Phosphorylation
K861 Acetylation
K861 Ubiquitination
Y864 Phosphorylation
Y872 Phosphorylation
S880 Phosphorylation
S882 Phosphorylation
S883 Phosphorylation
S885 Phosphorylation
S886 Phosphorylation Q00535 (CDK5)
T888 Phosphorylation
S891 Phosphorylation
T898 Phosphorylation
K902 Ubiquitination
K907 Ubiquitination
S910 Phosphorylation O14757 (CHEK1)
K925 Ubiquitination
K939 Ubiquitination
K943 Ubiquitination
S944 Phosphorylation
Y950 Phosphorylation
K951 Acetylation
S954 Phosphorylation
K980 Ubiquitination
S990 Phosphorylation
K991 Methylation
K991 Ubiquitination
S998 Phosphorylation
S999 Phosphorylation
S1000 Phosphorylation
K1009 Ubiquitination
T1012 Phosphorylation
K1019 Ubiquitination
K1020 Ubiquitination
K1069 Ubiquitination
K1070 Ubiquitination
K1091 Ubiquitination
K1109 Ubiquitination
T1121 Phosphorylation
K1132 Ubiquitination
K1143 Ubiquitination
R1152 Methylation
K1156 Acetylation
K1156 Ubiquitination
K1207 Ubiquitination
Y1221 Phosphorylation
T1222 Phosphorylation
K1250 Methylation
R1278 Methylation
T1279 Phosphorylation
S1336 Phosphorylation
Y1349 Phosphorylation
S1350 Phosphorylation
K1354 Methylation
K1361 Ubiquitination
K1375 Ubiquitination
C1377 S-Nitrosylation
K1389 Acetylation
K1389 Ubiquitination
K1435 Ubiquitination
K1458 Ubiquitination
K1477 Ubiquitination
C1480 S-Nitrosylation
K1484 Ubiquitination
C1487 S-Nitrosylation
K1503 Acetylation
K1503 Ubiquitination
R1510 Methylation

研究背景

機能:

Multifunctional protein which is primarily part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex, that catalyzes the attachment of the cognate amino acid to the corresponding tRNA in a two-step reaction: the amino acid is first activated by ATP to form a covalent intermediate with AMP and is then transferred to the acceptor end of the cognate tRNA. The phosphorylation of EPRS1, induced by interferon-gamma, dissociates the protein from the aminoacyl-tRNA synthetase multienzyme complex and recruits it to the GAIT complex that binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin), suppressing their translation. Interferon-gamma can therefore redirect, in specific cells, the EPRS1 function from protein synthesis to translation inhibition. Also functions as an effector of the mTORC1 signaling pathway by promoting, through SLC27A1, the uptake of long-chain fatty acid by adipocytes. Thereby, it also plays a role in fat metabolism and more indirectly influences lifespan.

PTMs:

Phosphorylated at Ser-886 by CDK5. Phosphorylated at Ser-999 by RPS6KB1; triggers EPRS1 release from the aminoacyl-tRNA synthetase multienzyme complex. In monocytes, the IFN-gamma-induced sequential phosphorylation at Ser-886 and Ser-999 releases EPRS1 from the aminoacyl-tRNA synthetase multienzyme complex, allowing its association with the GAIT complex. Phosphorylation at Ser-999 is specifically required for the RPL13A-mediated interaction of the GAIT complex with eIF4G. Phosphorylation at Ser-999 by RPS6KB1, is also induced by insulin through activation of the mTORC1 signaling pathway and promotes the interaction of EPRS1 with SLC27A1.

細胞の位置付け:

Cytoplasm>Cytosol. Membrane>Peripheral membrane protein.
Note: Translocates from cytosol to membranes upon phosphorylation at Ser-999.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homodimer. Part of the aminoacyl-tRNA synthetase multienzyme complex, also know as multisynthetase complex (MSC), that is composed of the tRNA ligases for Arg (RARS1), Asp (DARS1), Gln (QARS1), Ile (IARS1), Leu (LARS1), Lys (KARS1), Met (MARS1) the bifunctional ligase for Glu and Pro (EPRS1) and the auxiliary subunits AIMP1/p43, AIMP2/p38 and EEF1E1/p18. Forms a linear complex that contains MARS1, EEF1E1, EPRS1 and AIMP2 that is at the core of the multisubunit complex. Interacts with TARS3. Interacts with DUS2L. Component of the GAIT complex which is composed of EPRS1, RPL13A and GAPDH. For human, the complex assembly seems to be a two-step process in which EPRS1 first associates with SYNCRIP to form a pre-GAIT complex which is deficient in GAIT element binding. Interacts (phosphorylated at Ser-999) with SLC27A1; mediates the translocation of SLC27A1 from the cytoplasm to the plasma membrane thereby increasing the uptake of long-chain fatty acids.

タンパク質ファミリー:

The WHEP-TRS domains are involved in RNA binding.

In the N-terminal section; belongs to the class-I aminoacyl-tRNA synthetase family. Glutamate--tRNA ligase type 2 subfamily.

In the C-terminal section; belongs to the class-II aminoacyl-tRNA synthetase family.

研究領域

· Genetic Information Processing > Translation > Aminoacyl-tRNA biosynthesis.

· Metabolism > Metabolism of cofactors and vitamins > Porphyrin and chlorophyll metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

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