製品: Phospho-ATG4B (Ser316) Antibody
カタログ: AF3505
タンパク質の説明: Rabbit polyclonal antibody to Phospho-ATG4B (Ser316)
アプリケーション: ELISA(peptide)
反応性: Human, Mouse
分子量: 44kD(Calculated).
ユニプロット: Q9Y4P1
RRID: AB_2846819

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製品説明

ソース:
Rabbit
アプリケーション:
ELISA(peptide) 1:20000-1:40000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
Phospho-ATG4B (Ser316) Antibody detects endogenous levels of ATG4B only when phosphorylated at Ser316.
RRID:
AB_2846819
引用形式: Affinity Biosciences Cat# AF3505, RRID:AB_2846819.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

APG4 autophagy 4 homolog B; APG4B; ATG4 autophagy related 4 homolog B (S. cerevisiae); ATG4B; ATG4B_HUMAN; AUT like 1 cysteine endopeptidase; AUT-like 1 cysteine endopeptidase; AUTL1; Autophagin 1; Autophagin-1; Autophagy related 4B cysteine peptidase; Autophagy related cysteine endopeptidase 1; Autophagy related protein 4 homolog B; Autophagy-related cysteine endopeptidase 1; Autophagy-related protein 4 homolog B; Cysteine protease ATG4B; hAPG4B; MGC1353;

免疫原

免疫原:

A synthesized peptide derived from human ATG4B around the phosphorylation site of Ser316.

Uniprot:
遺伝子(ID):
発現特異性:
Q9Y4P1 ATG4B_HUMAN:

Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.

タンパク質配列:
MDAATLTYDTLRFAEFEDFPETSEPVWILGRKYSIFTEKDEILSDVASRLWFTYRKNFPAIGGTGPTSDTGWGCMLRCGQMIFAQALVCRHLGRDWRWTQRKRQPDSYFSVLNAFIDRKDSYYSIHQIAQMGVGEGKSIGQWYGPNTVAQVLKKLAVFDTWSSLAVHIAMDNTVVMEEIRRLCRTSVPCAGATAFPADSDRHCNGFPAGAEVTNRPSPWRPLVLLIPLRLGLTDINEAYVETLKHCFMMPQSLGVIGGKPNSAHYFIGYVGEELIYLDPHTTQPAVEPTDGCFIPDESFHCQHPPCRMSIAELDPSIAVGFFCKTEDDFNDWCQQVKKLSLLGGALPMFELVELQPSHLACPDVLNLSLDSSDVERLERFFDSEDEDFEILSL

PTMs - Q9Y4P1 基板として

Site PTM Type Enzyme
M1 Acetylation
Y8 Phosphorylation
S34 Phosphorylation
K39 Ubiquitination
S44 Phosphorylation
K102 Ubiquitination
K119 Ubiquitination
Y122 Phosphorylation
Y123 Phosphorylation
K153 Ubiquitination
S316 Phosphorylation
K337 Ubiquitination
S383 Phosphorylation
S392 Phosphorylation

研究背景

機能:

Cysteine protease required for the cytoplasm to vacuole transport (Cvt) and autophagy. Cleaves the C-terminal amino acid of ATG8 family proteins MAP1LC3, GABARAPL1, GABARAPL2 and GABARAP, to reveal a C-terminal glycine. Exposure of the glycine at the C-terminus is essential for ATG8 proteins conjugation to phosphatidylethanolamine (PE) and insertion to membranes, which is necessary for autophagy. Has also an activity of delipidating enzyme for the PE-conjugated forms.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Mainly expressed in the skeletal muscle, followed by brain, heart, liver and pancreas.

タンパク質ファミリー:

Belongs to the peptidase C54 family.

研究領域

· Cellular Processes > Transport and catabolism > Autophagy - other.   (View pathway)

· Cellular Processes > Transport and catabolism > Autophagy - animal.   (View pathway)

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