SPC25 Antibody - #DF13747

(1)   (3)   Copy Info
製品: SPC25 Antibody
カタログ: DF13747
タンパク質の説明: Rabbit polyclonal antibody to SPC25
アプリケーション: WB
Cited expt.: WB
反応性: Human, Mouse
予測: Bovine, Horse, Rabbit, Dog
分子量: 26kDa(Observed); 26kD(Calculated).
ユニプロット: Q9HBM1
RRID: AB_2846766

類似製品を見る>>

   サイズ 価格 在庫状況
 100ul $280 在庫あり
 200ul $350 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先
Control Products

#DF13747-BP

SPC25 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Bovine(88%), Horse(88%), Rabbit(88%), Dog(88%)
クローナリティ:
Polyclonal
特異性:
SPC25 Antibody detects endogenous levels of total SPC25.
RRID:
AB_2846766
引用形式: Affinity Biosciences Cat# DF13747, RRID:AB_2846766.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AD024; hSpc25; Kinetochore protein Spc25; SPBC25; SPC25; SPC25_HUMAN; spindle pole body component 25 homolog;

免疫原

免疫原:

A synthesized peptide derived from human SPC25, corresponding to a region within N-terminal amino acids.

Uniprot:

Q9HBM1(SPC25_HUMAN) >>Visit HPA database.

遺伝子(ID):
SPC25(57405)
タンパク質配列:
MVEDELALFDKSINEFWNKFKSTDTSCQMAGLRDTYKDSIKAFAEKLSVKLKEEERMVEMFLEYQNQISRQNKLIQEKKDNLLKLIAEVKGKKQELEVLTANIQDLKEEYSRKKETISTANKANAERLKRLQKSADLYKDRLGLEIRKIYGEKLQFIFTNIDPKNPESPFMFSLHLNEARDYEVSDSAPHLEGLAEFQENVRKTNNFSAFLANVRKAFTATVYN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
88
Bovine
88
Dog
88
Rabbit
88
Pig
75
Sheep
75
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Acts as a component of the essential kinetochore-associated NDC80 complex, which is required for chromosome segregation and spindle checkpoint activity. Required for kinetochore integrity and the organization of stable microtubule binding sites in the outer plate of the kinetochore. The NDC80 complex synergistically enhances the affinity of the SKA1 complex for microtubules and may allow the NDC80 complex to track depolymerizing microtubules.

細胞の位置付け:

Nucleus. Chromosome>Centromere>Kinetochore.
Note: Localizes to kinetochores from late prophase to anaphase (PubMed:14738735). Localizes specifically to the outer plate of the kinetochore (PubMed:14738735, PubMed:14699129).

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the SPC25 family.

参考文献

1). SPC25 as a novel therapeutic and prognostic biomarker and its association with glycolysis, ferroptosis and ceRNA in lung adenocarcinoma. Aging (Albany NY), 2024 (PubMed: 38217547)

Application: WB    Species: human    Sample: H1975 cells

Figure 3. The impact of knocking down SPC25 on LUAD. (A, B) An analysis of Western blotting confirmed the success of SPC25-siRNAs transfection into H1975 cells and their ability to interfere with gene expression. (C, D) CCK-8 assay evaluated tumor cell proliferation activity. (E, F) EdU staining of H1975 cells and EdU-positive cell proportion. (G, H) The effect of SPC25 knockdown on apoptosis of the H1975 cells as detected by flow cytometry. (I–K) Representative images and quantitative analysis of wound healing measurements in LUAD cells. (***p < 0.001; **p < 0.01; *p < 0.05; Abbreviation: ns: not significant).

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.