製品: TAP1 Antibody
カタログ: DF13735
タンパク質の説明: Rabbit polyclonal antibody to TAP1
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Monkey
分子量: 87kDa; 87kD(Calculated).
ユニプロット: Q03518
RRID: AB_2846754

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製品説明

ソース:
Rabbit
アプリケーション:
IF/ICC 1:100-1:500, WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Monkey
クローナリティ:
Polyclonal
特異性:
TAP1 Antibody detects endogenous levels of total TAP1.
RRID:
AB_2846754
引用形式: Affinity Biosciences Cat# DF13735, RRID:AB_2846754.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

ABC 17; ABC transporter MHC 1; ABC17; ABCB 2; ABCB2; Antigen peptide transporter 1; APT 1; APT1; ATP binding cassette sub family B (MDR/TAP) member 2; ATP binding cassette sub family B member 2; ATP binding cassette transporter; ATP-binding cassette sub-family B member 2; D6S114E; FLJ26666; FLJ41500; Peptide supply factor 1; Peptide transporter involved in antigen processing 1; Peptide transporter PSF 1; Peptide transporter PSF1; Peptide transporter TAP 1; Peptide transporter TAP1; PSF 1; PSF-1; PSF1; Really interesting new gene 4 protein; RING 4; RING4; TAP 1; TAP1; TAP1*0102N; TAP1_HUMAN; TAP1N; Transporter 1 ATP binding cassette sub family B (MDR/TAP); Transporter 1 ATP binding cassette sub family B; Transporter associated with antigen processing; Transporter ATP binding cassette major histocompatibility complex 1; Y3;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MAELLASAGSACSWDFPRAPPSFPPPAASRGGLGGTRSFRPHRGAESPRPGRDRDGVRVPMASSRCPAPRGCRCLPGASLAWLGTVLLLLADWVLLRTALPRIFSLLVPTALPLLRVWAVGLSRWAVLWLGACGVLRATVGSKSENAGAQGWLAALKPLAAALGLALPGLALFRELISWGAPGSADSTRLLHWGSHPTAFVVSYAAALPAAALWHKLGSLWVPGGQGGSGNPVRRLLGCLGSETRRLSLFLVLVVLSSLGEMAIPFFTGRLTDWILQDGSADTFTRNLTLMSILTIASAVLEFVGDGIYNNTMGHVHSHLQGEVFGAVLRQETEFFQQNQTGNIMSRVTEDTSTLSDSLSENLSLFLWYLVRGLCLLGIMLWGSVSLTMVTLITLPLLFLLPKKVGKWYQLLEVQVRESLAKSSQVAIEALSAMPTVRSFANEEGEAQKFREKLQEIKTLNQKEAVAYAVNSWTTSISGMLLKVGILYIGGQLVTSGAVSSGNLVTFVLYQMQFTQAVEVLLSIYPRVQKAVGSSEKIFEYLDRTPRCPPSGLLTPLHLEGLVQFQDVSFAYPNRPDVLVLQGLTFTLRPGEVTALVGPNGSGKSTVAALLQNLYQPTGGQLLLDGKPLPQYEHRYLHRQVAAVGQEPQVFGRSLQENIAYGLTQKPTMEEITAAAVKSGAHSFISGLPQGYDTEVDEAGSQLSGGQRQAVALARALIRKPCVLILDDATSALDANSQLQVEQLLYESPERYSRSVLLITQHLSLVEQADHILFLEGGAIREGGTHQQLMEKKGCYWAMVQAPADAPE

PTMs - Q03518 基板として

Site PTM Type Enzyme
R30 Methylation
R37 Methylation
S47 Phosphorylation
K422 Ubiquitination
S424 Phosphorylation
S439 Phosphorylation
K449 Ubiquitination
K453 Ubiquitination
K458 Ubiquitination
K537 Ubiquitination
Y541 Phosphorylation
T545 Phosphorylation
T587 Phosphorylation
K627 Ubiquitination
K666 Ubiquitination
T694 Phosphorylation

研究背景

機能:

Involved in the transport of antigens from the cytoplasm to the endoplasmic reticulum for association with MHC class I molecules. Also acts as a molecular scaffold for the final stage of MHC class I folding, namely the binding of peptide. Nascent MHC class I molecules associate with TAP via tapasin. Inhibited by the covalent attachment of herpes simplex virus ICP47 protein, which blocks the peptide-binding site of TAP. Inhibited by human cytomegalovirus US6 glycoprotein, which binds to the lumenal side of the TAP complex and inhibits peptide translocation by specifically blocking ATP-binding to TAP1 and prevents the conformational rearrangement of TAP induced by peptide binding. Inhibited by human adenovirus E3-19K glycoprotein, which binds the TAP complex and acts as a tapasin inhibitor, preventing MHC class I/TAP association. Expression of TAP1 is down-regulated by human Epstein-Barr virus vIL-10 protein, thereby affecting the transport of peptides into the endoplasmic reticulum and subsequent peptide loading by MHC class I molecules.

細胞の位置付け:

Endoplasmic reticulum membrane>Multi-pass membrane protein.
Note: The transmembrane segments seem to form a pore in the membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Heterodimer of TAP1 and TAP2. Interacts with HLA-A*02-B2M complex; this interaction is obligatory for the loading of peptide epitopes delivered to the ER by TAP1-TAP2 transporter. Interacts with PSMB5 and PSMB8. Similarly to classical MHC class I assembly, HLA-E-B2M complex binds TAP1-TAP2 heterodimer and CALR before the peptide loading. Interacts with HLA-F. Interacts with PSMB5 and PSMB8.

(Microbial infection) Interacts with Epstein-Barr virus BNLF2a.

(Microbial infection) Interacts with herpes simplex virus ICP47.

タンパク質ファミリー:

The peptide-binding site is shared between the cytoplasmic loops of TAP1 and TAP2.

Belongs to the ABC transporter superfamily. ABCB family. MHC peptide exporter (TC 3.A.1.209) subfamily.

研究領域

· Cellular Processes > Transport and catabolism > Phagosome.   (View pathway)

· Environmental Information Processing > Membrane transport > ABC transporters.

· Human Diseases > Infectious diseases: Viral > Herpes simplex infection.

· Human Diseases > Immune diseases > Primary immunodeficiency.

· Organismal Systems > Immune system > Antigen processing and presentation.   (View pathway)

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