LOXL4 Antibody - #DF13661

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製品: LOXL4 Antibody
カタログ: DF13661
タンパク質の説明: Rabbit polyclonal antibody to LOXL4
アプリケーション: WB IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 84kDa(Observed); 84kD(Calculated).
ユニプロット: Q96JB6
RRID: AB_2846680

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#DF13661-BP

LOXL4 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(83%), Bovine(100%), Horse(88%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
LOXL4 Antibody detects endogenous levels of total LOXL4.
RRID:
AB_2846680
引用形式: Affinity Biosciences Cat# DF13661, RRID:AB_2846680.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

4833426I20Rik; FLJ21889; LOX L4; LOXC; LOXL 4; LOXL4; LOXL4_HUMAN; Lysyl oxidase homolog 4; Lysyl oxidase like 4; Lysyl oxidase like protein 4; Lysyl oxidase related C; Lysyl oxidase related protein C; Lysyl oxidase-like protein 4; Lysyl oxidase-related protein C;

免疫原

免疫原:

A synthesized peptide derived from human LOXL4, corresponding to a region within N-terminal amino acids.

Uniprot:

Q96JB6(LOXL4_HUMAN) >>Visit HPA database.

遺伝子(ID):
LOXL4(84171)
発現特異性:
Q96JB6 LOXL4_HUMAN:

Expressed in many tissues, the highest levels among the tissues studied being in the skeletal muscle, testis and pancreas. Expressed in cartilage.

タンパク質配列:
MAWSPPATLFLFLLLLGQPPPSRPQSLGTTKLRLVGPESKPEEGRLEVLHQGQWGTVCDDNFAIQEATVACRQLGFEAALTWAHSAKYGQGEGPIWLDNVRCVGTESSLDQCGSNGWGVSDCSHSEDVGVICHPRRHRGYLSETVSNALGPQGRRLEEVRLKPILASAKQHSPVTEGAVEVKYEGHWRQVCDQGWTMNNSRVVCGMLGFPSEVPVDSHYYRKVWDLKMRDPKSRLKSLTNKNSFWIHQVTCLGTEPHMANCQVQVAPARGKLRPACPGGMHAVVSCVAGPHFRPPKTKPQRKGSWAEEPRVRLRSGAQVGEGRVEVLMNRQWGTVCDHRWNLISASVVCRQLGFGSAREALFGARLGQGLGPIHLSEVRCRGYERTLSDCPALEGSQNGCQHENDAAVRCNVPNMGFQNQVRLAGGRIPEEGLLEVQVEVNGVPRWGSVCSENWGLTEAMVACRQLGLGFAIHAYKETWFWSGTPRAQEVVMSGVRCSGTELALQQCQRHGPVHCSHGGGRFLAGVSCMDSAPDLVMNAQLVQETAYLEDRPLSQLYCAHEENCLSKSADHMDWPYGYRRLLRFSTQIYNLGRTDFRPKTGRDSWVWHQCHRHYHSIEVFTHYDLLTLNGSKVAEGHKASFCLEDTNCPTGLQRRYACANFGEQGVTVGCWDTYRHDIDCQWVDITDVGPGNYIFQVIVNPHYEVAESDFSNNMLQCRCKYDGHRVWLHNCHTGNSYPANAELSLEQEQRLRNNLI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Horse
88
Zebrafish
83
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

May modulate the formation of a collagenous extracellular matrix.

PTMs:

The lysine tyrosylquinone cross-link (LTQ) is generated by condensation of the epsilon-amino group of a lysine with a topaquinone produced by oxidation of tyrosine.

細胞の位置付け:

Secreted>Extracellular space.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in many tissues, the highest levels among the tissues studied being in the skeletal muscle, testis and pancreas. Expressed in cartilage.

タンパク質ファミリー:

Belongs to the lysyl oxidase family.

参考文献

1). Deficiency of Integrin β 4 Results in Increased Lung Tissue Stiffness and Responds to Substrate Stiffness via Modulating RhoA Activity. Frontiers in cell and developmental biology, 2022 (PubMed: 35309934) [IF=4.6]

Application: IHC    Species: Mouse    Sample:

FIGURE 3. The expression of collagens, elastin and LOXs between ITGB4f/f and ITGB4ccsp.cre mice. (A,B) Masson’s Trichrome stain image (a, b) and ImageJ Imaging Software-generated image (c, d) to calculate Area % of collagen (Red). Scale bars: 50 μm. (C,D) The mRNA expression levels of collagens, elastin and LOXs were detected by RT-PCR. (E) Immunostaining for LOXL4 in airway epithelium (a,b) and alveolar septa (c,d) in ITGB4f/fand ITGB4ccsp.crelungs. Scale bars: 50 μm for (a, b);20 μm for (c, d). (F) Immunostaining for COLVI in pulmonary alveoli (a–d) and in airway epithelium (e–h) in ITGB4f/f and ITGB4ccsp.crelungs. Scale bars: 200 μm for (a, b, e, f); 50 μm for (c, d, g, h). (G) Immunostaining for elastin in pulmonary alveoli. Scale bars: 20 μm. (n = 3 independent experiments, *p < 0.05, **p < 0.01). Data are presented as mean ± SD.

Application: IF/ICC    Species: Mouse    Sample:

FIGURE 4. The expression of LOXL4,ColⅥ and elastin between ITGB4f/f and. ITGB4ccsp.cre mice (A) Immunofluorescence images of Col VI (green) and LOXL4 (pink) in the airway epithelium (a, b) and the Col VI (green) and elastin (red) in alveoli (c, d). Scale bars: 50 μm. (B) Immunofluorescence images of elastin in the in alveoli. Scale bars: 20 μm. Lung elastin accumulation (arrows) was distinctly greater in the ITGB4ccsp.cre lungs.
2). Pathological changes and expression of lysine oxidases and matrix metalloproteinases‐1,‐2, and‐3 in ligaments of patients with haemophilic arthritis. HAEMOPHILIA, 2022 (PubMed: 34697874) [IF=3.0]

Application: WB    Species: human    Sample: cruciate ligaments

Figure 5.|LOXs and MMP-1,-2,and -3protein expression levels in the cruciate ligaments of patients with OA or HA

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