製品: MIS12 Antibody
カタログ: DF13646
タンパク質の説明: Rabbit polyclonal antibody to MIS12
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Bovine
分子量: 24kDa; 24kD(Calculated).
ユニプロット: Q9H081
RRID: AB_2846665

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(83%)
クローナリティ:
Polyclonal
特異性:
MIS12 Antibody detects endogenous levels of total MIS12.
RRID:
AB_2846665
引用形式: Affinity Biosciences Cat# DF13646, RRID:AB_2846665.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

hMIS12; homolog of yeast Mis12; KNTC2AP; MIND kinetochore complex component, homolog (yeast); MIS12 homolog (yeast); MIS12 homolog; MIS12 MIND kinetochore complex component homolog (yeast); MTW 1; MTW1; Protein MIS12 homolog;

免疫原

免疫原:

A synthesized peptide derived from human MIS12, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
タンパク質配列:
MSVDPMTYEAQFFGFTPQTCMLRIYIAFQDYLFEVMQAVEQVILKKLDGIPDCDISPVQIRKCTEKFLCFMKGHFDNLFSKMEQLFLQLILRIPSNILLPEDKCKETPYSEEDFQHLQKEIEQLQEKYKTELCTKQALLAELEEQKIVQAKLKQTLTFFDELHNVGRDHGTSDFRESLVSLVQNSRKLQNIRDNVEKESKRLKIS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
83
Horse
75
Chicken
71
Sheep
67
Xenopus
57
Pig
0
Dog
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Part of the MIS12 complex which is required for normal chromosome alignment and segregation and for kinetochore formation during mitosis. Essential for proper kinetochore microtubule attachments.

細胞の位置付け:

Chromosome>Centromere>Kinetochore.
Note: Associated with the kinetochore.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the mis12 family.

Restrictive clause

 

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For Research Use Only.
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