Asporin Antibody - #DF13642

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製品: Asporin Antibody
カタログ: DF13642
タンパク質の説明: Rabbit polyclonal antibody to Asporin
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 43kDa, 37 kDa; 43kD(Calculated).
ユニプロット: Q9BXN1
RRID: AB_2846661

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(88%), Dog(100%), Xenopus(86%)
クローナリティ:
Polyclonal
特異性:
Asporin Antibody detects endogenous levels of total Asporin.
RRID:
AB_2846661
引用形式: Affinity Biosciences Cat# DF13642, RRID:AB_2846661.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

ASPN; ASPN protein; ASPN_HUMAN; Asporin (LRR class 1); Asporin; Asporin proteoglycan; FLJ20129; LRR class 1; Periodontal ligament associated protein 1; Periodontal ligament-associated protein 1; PLAP 1; PLAP-1; PLAP1; SLRR 1C; SLRR1C; Small leucine rich protein 1C;

免疫原

免疫原:

A synthesized peptide derived from human Asporin, corresponding to a region within C-terminal amino acids.

Uniprot:

Q9BXN1(ASPN_HUMAN) >>Visit HPA database.

遺伝子(ID):
ASPN(54829)
発現特異性:
Q9BXN1 ASPN_HUMAN:

Higher levels in osteoarthritic articular cartilage, aorta, uterus. Moderate expression in small intestine, heart, liver, bladder, ovary, stomach, and in the adrenal, thyroid, and mammary glands. Low expression in trachea, bone marrow, and lung. Colocalizes with TGFB1 in chondrocytes within osteoarthritic (OA) lesions of articular cartilage.

タンパク質配列:
MKEYVLLLFLALCSAKPFFSPSHIALKNMMLKDMEDTDDDDDDDDDDDDDDEDNSLFPTREPRSHFFPFDLFPMCPFGCQCYSRVVHCSDLGLTSVPTNIPFDTRMLDLQNNKIKEIKENDFKGLTSLYGLILNNNKLTKIHPKAFLTTKKLRRLYLSHNQLSEIPLNLPKSLAELRIHENKVKKIQKDTFKGMNALHVLEMSANPLDNNGIEPGAFEGVTVFHIRIAEAKLTSVPKGLPPTLLELHLDYNKISTVELEDFKRYKELQRLGLGNNKITDIENGSLANIPRVREIHLENNKLKKIPSGLPELKYLQIIFLHSNSIARVGVNDFCPTVPKMKKSLYSAISLFNNPVKYWEMQPATFRCVLSRMSVQLGNFGM

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
88
Xenopus
86
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Negatively regulates periodontal ligament (PDL) differentiation and mineralization to ensure that the PDL is not ossified and to maintain homeostasis of the tooth-supporting system. Inhibits BMP2-induced cytodifferentiation of PDL cells by preventing its binding to BMPR1B/BMP type-1B receptor, resulting in inhibition of BMP-dependent activation of SMAD proteins (By similarity). Critical regulator of TGF-beta in articular cartilage and plays an essential role in cartilage homeostasis and osteoarthritis (OA) pathogenesis. Negatively regulates chondrogenesis in the articular cartilage by blocking the TGF-beta/receptor interaction on the cell surface and inhibiting the canonical TGF-beta/Smad signal. Binds calcium and plays a role in osteoblast-driven collagen biomineralization activity.

PTMs:

There is no serine/glycine dipeptide sequence expected for the attachment of O-linked glycosaminoglycans and this is probably not a proteoglycan. The O-linked polysaccharide on 54-Ser is probably the mucin type linked to GalNAc.

The N-linked glycan at Asn-282 is composed of variable structures of GlcNAc, mannose, fucose, HexNAc and hexose.

細胞の位置付け:

Secreted>Extracellular space>Extracellular matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Higher levels in osteoarthritic articular cartilage, aorta, uterus. Moderate expression in small intestine, heart, liver, bladder, ovary, stomach, and in the adrenal, thyroid, and mammary glands. Low expression in trachea, bone marrow, and lung. Colocalizes with TGFB1 in chondrocytes within osteoarthritic (OA) lesions of articular cartilage.

タンパク質ファミリー:

The LRR 5 repeat can inhibit BMP2-induced cytodifferentiation and may be involved in the interaction with BMP2 (By similarity). The repeats LRR 10, LRR 11 and LRR 12 are involved in binding type I collagen. The poly-Asp region is involved in binding calcium.

Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily.

参考文献

1). Identification of nanoparticle-mediated siRNA-ASPN as a key gene target in the treatment of keloids. Frontiers in bioengineering and biotechnology, 2022 (PubMed: 36394011) [IF=4.3]

Application: WB    Species: human    Sample: skin tissue

FIGURE 4. (A) The SVM dimensionality reduction algorithm. (B) The Venn diagrams shows the six specific genes. (C) The boxplot showed significantly higher expression of ASPN in the keloid group than in normal skin. (D) The area under the ROC curve is 0.984 in analyzing the sensitivity and specificity of ASPN gene. (E–G) Specific expression of ASPN in keloids was confirmed by RT-qPCR and Western blot analysis, ASPN was strongly expressed in keloids but the expression signal was negative or weakly positive in normal skin tissue.
2). A transcriptome sequencing study on the effect of macro-pores in hydrogel scaffolds on global gene expression of laden human cartilage chondrocytes. Biomedical Materials, 2022 (PubMed: 35609582) [IF=3.9]

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