Repulsive Guidance Molecule A Antibody - #DF13622

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製品: Repulsive Guidance Molecule A Antibody
カタログ: DF13622
タンパク質の説明: Rabbit polyclonal antibody to Repulsive Guidance Molecule A
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog, Chicken
分子量: 49kDa(Observed); 49kD(Calculated).
ユニプロット: Q96B86
RRID: AB_2846641

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#DF13622-BP

Repulsive Guidance Molecule A Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
Repulsive Guidance Molecule A Antibody detects endogenous levels of total Repulsive Guidance Molecule A.
RRID:
AB_2846641
引用形式: Affinity Biosciences Cat# DF13622, RRID:AB_2846641.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Repulsive guidance molecule A; RGM; RGM domain family member A; RGMA; RGMA_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human Repulsive Guidance Molecule A, corresponding to a region within the internal amino acids.

Uniprot:

Q96B86(RGMA_HUMAN) >>Visit HPA database.

遺伝子(ID):
RGMA(56963)
タンパク質配列:
MQPPRERLVVTGRAGWMGMGRGAGRSALGFWPTLAFLLCSFPAATSPCKILKCNSEFWSATSGSHAPASDDTPEFCAALRSYALCTRRTARTCRGDLAYHSAVHGIEDLMSQHNCSKDGPTSQPRLRTLPPAGDSQERSDSPEICHYEKSFHKHSATPNYTHCGLFGDPHLRTFTDRFQTCKVQGAWPLIDNNYLNVQVTNTPVLPGSAATATSKLTIIFKNFQECVDQKVYQAEMDELPAAFVDGSKNGGDKHGANSLKITEKVSGQHVEIQAKYIGTTIVVRQVGRYLTFAVRMPEEVVNAVEDWDSQGLYLCLRGCPLNQQIDFQAFHTNAEGTGARRLAAASPAPTAPETFPYETAVAKCKEKLPVEDLYYQACVFDLLTTGDVNFTLAAYYALEDVKMLHSNKDKLHLYDRTRDLPGRAAAGLPLAPRPLLGALVPLLALLPVFC

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Zebrafish
63
Xenopus
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Member of the repulsive guidance molecule (RGM) family that performs several functions in the developing and adult nervous system. Regulates cephalic neural tube closure, inhibits neurite outgrowth and cortical neuron branching, and the formation of mature synapses. Binding to its receptor NEO1/neogenin induces activation of RHOA-ROCK1/Rho-kinase signaling pathway through UNC5B-ARHGEF12/LARG-PTK2/FAK1 cascade, leading to collapse of the neuronal growth cone and neurite outgrowth inhibition. Furthermore, RGMA binding to NEO1/neogenin leads to HRAS inactivation by influencing HRAS-PTK2/FAK1-AKT1 pathway. It also functions as a bone morphogenetic protein (BMP) coreceptor that may signal through SMAD1, SMAD5, and SMAD8.

PTMs:

Autocatalytically cleaved at low pH; the two chains remain linked via two disulfide bonds.

細胞の位置付け:

Cell membrane>Lipid-anchor.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the repulsive guidance molecule (RGM) family.

参考文献

1). PTBP1 knockdown promotes neural differentiation of glioblastoma cells through UNC5B receptor. Theranostics, 2022 (PubMed: 35664063) [IF=12.4]

Application: WB    Species: Human    Sample: U251 cells

Figure 8 A RhoA inhibitor prevents the PTBP1 knockdown induced reprogramming. (A-C) Western blot study of NTN4 and RGMA protein in U251 cells infected with sh-Luci and sh-PTBP1 for 3 and 7 days. (n = 3). GAPDH was used as an internal reference protein. (D) RhoA activity measured by G-LISA. (n = 3). (E-G) TUJ1 and KI67 positive rates of five distinct groups constituted of lentivirus (sh-Luci or sh-PTBP1) and Rhosin (vehicle, 10 µM, and 30 µM; nine random fields from triplicate samples were recorded for quantification; TUJ1+ (%) = TUJ1+ M-cherry+/M-cherry+; KI67+ (%) = KI67+ M-cherry+/M-cherry+; M-cherry+ cells = 286-538 for each condition). (H) Pathways involved in PTBP1 knockdown-induced reprogramming. The data are presented as mean ± SD. **P < 0.01, *** P < 0.001 vs. sh-Luci-3d or sh-PTBP1 + vehicle group. Dpi (d): days post infection; ND: not detected; NS: no significance. Scale: 100 µm.
2). miR-106b-5p upregulation is associated with microglial activation and inflammation in the mouse hippocampus following status epilepticus. EXPERIMENTAL BRAIN RESEARCH, 2021 (PubMed: 34476536) [IF=1.7]

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