SET Antibody - #DF13529

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製品: SET Antibody
カタログ: DF13529
タンパク質の説明: Rabbit polyclonal antibody to SET
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human, Rat
分子量: 33kDa(Observed); 33kD(Calculated).
ユニプロット: Q01105
RRID: AB_2846548

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#DF13529-BP

SET Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Rat
クローナリティ:
Polyclonal
特異性:
SET Antibody detects endogenous levels of total SET.
RRID:
AB_2846548
引用形式: Affinity Biosciences Cat# DF13529, RRID:AB_2846548.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

2PP2A; HLA DR associated protein II; HLA-DR-associated protein II; I 2PP2A; I-2PP2A; I2PP2A; IGAAD; Inhibitor of granzyme A activated DNase; Inhibitor of granzyme A-activated DNase; Inhibitor of GzmA-activated DNase; inhibitor-2 of protein phosphatase-2A; IPP2A2; PHAPII; Phosphatase 2A inhibitor I2PP2A; protein phosphatase type 2A inhibitor; Protein SET; Set; SET nuclear oncogene; SET translocation; SET translocation (myeloid leukemia-associated); SET_HUMAN; TAF I; TAF IBETA; TAF-I; TAFI; Template activating factor I; Template-activating factor I; Template-Activating Factor-I, chromatin remodelling factor;

免疫原

免疫原:

A synthesized peptide derived from human SET, corresponding to a region within N-terminal amino acids.

Uniprot:

Q01105(SET_HUMAN) >>Visit HPA database.

遺伝子(ID):
SET(6418)
発現特異性:
Q01105 SET_HUMAN:

Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.

タンパク質配列:
MAPKRQSPLPPQKKKPRPPPALGPEETSASAGLPKKGEKEQQEAIEHIDEVQNEIDRLNEQASEEILKVEQKYNKLRQPFFQKRSELIAKIPNFWVTTFVNHPQVSALLGEEDEEALHYLTRVEVTEFEDIKSGYRIDFYFDENPYFENKVLSKEFHLNESGDPSSKSTEIKWKSGKDLTKRSSQTQNKASRKRQHEEPESFFTWFTDHSDAGADELGEVIKDDIWPNPLQYYLVPDMDDEEGEGEEDDDDDEEEEGLEDIDEEGDEDEGEEDEDDDEGEEGEEDEGEDD

研究背景

機能:

Multitasking protein, involved in apoptosis, transcription, nucleosome assembly and histone chaperoning. Isoform 2 anti-apoptotic activity is mediated by inhibition of the GZMA-activated DNase, NME1. In the course of cytotoxic T-lymphocyte (CTL)-induced apoptosis, GZMA cleaves SET, disrupting its binding to NME1 and releasing NME1 inhibition. Isoform 1 and isoform 2 are potent inhibitors of protein phosphatase 2A. Isoform 1 and isoform 2 inhibit EP300/CREBBP and PCAF-mediated acetylation of histones (HAT) and nucleosomes, most probably by masking the accessibility of lysines of histones to the acetylases. The predominant target for inhibition is histone H4. HAT inhibition leads to silencing of HAT-dependent transcription and prevents active demethylation of DNA. Both isoforms stimulate DNA replication of the adenovirus genome complexed with viral core proteins; however, isoform 2 specific activity is higher.

PTMs:

Isoform 2 is phosphorylated on Ser-15 and Ser-24.

Isoform 2 is acetylated on Lys-11.

Some glutamate residues are glycylated by TTLL8. This modification occurs exclusively on glutamate residues and results in a glycine chain on the gamma-carboxyl group (By similarity).

N-terminus of isoform 1 is methylated by METTL11A/NTM1. Mainly trimethylated (By similarity).

Cleaved after Lys-176 by GZMA. The cleavage inhibits its nucleosome assembly activity and disrupts the inhibition on NME1.

細胞の位置付け:

Cytoplasm>Cytosol. Endoplasmic reticulum. Nucleus>Nucleoplasm.
Note: In the cytoplasm, found both in the cytosol and associated with the endoplasmic reticulum. The SET complex is associated with the endoplasmic reticulum. Following CTL attack and cleavage by GZMA, moves rapidly to the nucleus, where it is found in the nucleoplasm, avoiding the nucleolus. Similar translocation to the nucleus is also observed for lymphocyte-activated killer cells after the addition of calcium.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed. Low levels in quiescent cells during serum starvation, contact inhibition or differentiation. Highly expressed in Wilms' tumor.

タンパク質ファミリー:

A long alpha helix in the N-terminus mediates dimerization, while the earmuff domain is responsible for core histone and dsDNA binding. The C-terminal acidic domain mediates the inhibition of histone acetyltransferases and is required for the DNA replication stimulatory activity.

Belongs to the nucleosome assembly protein (NAP) family.

参考文献

1). Sodium arsenite induces islets β-cells apoptosis and dysfunction via SET-Rac1-mediated cytoskeleton disturbance. Ecotoxicology and environmental safety, 2025 (PubMed: 39765117) [IF=6.2]

Application: WB    Species: Rat    Sample:

Fig. 2. The effect of NaAsO2 on the expression of SET and Rac1. Gavage was administered to SD rats for 5 months at a dosage of 5 mg/kg BW. The min6 cells were treated with NaAsO2 (4 μM) for 24 h. (A-C) Western blot images and quantifications of SET and Rac1 in min6 cells. (D-F) Western blot images and quantifications of SET and Rac1 in rat pancreatic tissues. (G and H) Immunofluorescence images of SET (G) and Rac1 (H) in rat islet β-cells. The data represent the X̅±SEM, and the Student’s two-tailed t-test was used to determine statistical significance, n = 3, *p 

Application: IF/ICC    Species: Rat    Sample:

Fig. 2. The effect of NaAsO2 on the expression of SET and Rac1. Gavage was administered to SD rats for 5 months at a dosage of 5 mg/kg BW. The min6 cells were treated with NaAsO2 (4 μM) for 24 h. (A-C) Western blot images and quantifications of SET and Rac1 in min6 cells. (D-F) Western blot images and quantifications of SET and Rac1 in rat pancreatic tissues. (G and H) Immunofluorescence images of SET (G) and Rac1 (H) in rat islet β-cells. The data represent the X̅±SEM, and the Student’s two-tailed t-test was used to determine statistical significance, n = 3, *p 

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