FOXA2 Antibody - #DF13363
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| 製品: | FOXA2 Antibody |
| カタログ: | DF13363 |
| タンパク質の説明: | Rabbit polyclonal antibody to FOXA2 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken |
| 分子量: | 48kDa; 48kD(Calculated). |
| ユニプロット: | Q9Y261 |
| RRID: | AB_2846382 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF13363, RRID:AB_2846382.
折りたたみ／展開
Forkhead box A2; Forkhead box protein A2; FOX A2; foxa2; FOXA2_HUMAN; Hepatic nuclear factor 3 beta; Hepatocyte nuclear factor 3; Hepatocyte nuclear factor 3 beta; Hepatocyte nuclear factor 3-beta; HNF 3B; HNF-3-beta; HNF-3B; HNF3B; MGC19807; TCF 3B; TCF-3B; TCF3B; Transcription factor 3B;
免疫原
- Q9Y261 FOXA2_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MLGAVKMEGHEPSDWSSYYAEPEGYSSVSNMNAGLGMNGMNTYMSMSAAAMGSGSGNMSAGSMNMSSYVGAGMSPSLAGMSPGAGAMAGMGGSAGAAGVAGMGPHLSPSLSPLGGQAAGAMGGLAPYANMNSMSPMYGQAGLSRARDPKTYRRSYTHAKPPYSYISLITMAIQQSPNKMLTLSEIYQWIMDLFPFYRQNQQRWQNSIRHSLSFNDCFLKVPRSPDKPGKGSFWTLHPDSGNMFENGCYLRRQKRFKCEKQLALKEAAGAAGSGKKAAAGAQASQAQLGEAAGPASETPAGTESPHSSASPCQEHKRGGLGELKGTPAAALSPPEPAPSPGQQQQAAAHLLGPPHHPGLPPEAHLKPEHHYAFNHPFSINNLMSSEQQHHHSHHHHQPHKMDLKAYEQVMHYPGYGSPMPGSLAMGPVTNKTGLDASPLAADTSYYQGVYSRPIMNSS
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - Q9Y261 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Acetylation | Uniprot | |
| S107 | Phosphorylation | O15111 (CHUK) | Uniprot |
| S111 | Phosphorylation | O15111 (CHUK) | Uniprot |
| T156 | Phosphorylation | P31749 (AKT1) | Uniprot |
| S212 | Phosphorylation | Uniprot | |
| S223 | Phosphorylation | Uniprot | |
| Y248 | Phosphorylation | Uniprot | |
| K256 | Acetylation | Uniprot | |
| K259 | Acetylation | Uniprot | |
| K264 | Acetylation | Uniprot | |
| K274 | Acetylation | Uniprot | |
| K275 | Acetylation | Uniprot | |
| S283 | Phosphorylation | Uniprot | |
| S295 | Phosphorylation | Uniprot | |
| T301 | Phosphorylation | Uniprot | |
| S303 | Phosphorylation | Uniprot | |
| S307 | Phosphorylation | Uniprot | |
| S309 | Phosphorylation | Uniprot |
研究背景
Transcription factor that is involved in embryonic development, establishment of tissue-specific gene expression and regulation of gene expression in differentiated tissues. Is thought to act as a 'pioneer' factor opening the compacted chromatin for other proteins through interactions with nucleosomal core histones and thereby replacing linker histones at target enhancer and/or promoter sites. Binds DNA with the consensus sequence 5'-[AC]A[AT]T[AG]TT[GT][AG][CT]T[CT]-3' (By similarity). In embryonic development is required for notochord formation. Involved in the development of multiple endoderm-derived organ systems such as the liver, pancreas and lungs; FOXA1 and FOXA2 seem to have at least in part redundant roles. Originally described as a transcription activator for a number of liver genes such as AFP, albumin, tyrosine aminotransferase, PEPCK, etc. Interacts with the cis-acting regulatory regions of these genes. Involved in glucose homeostasis; regulates the expression of genes important for glucose sensing in pancreatic beta-cells and glucose homeostasis. Involved in regulation of fat metabolism. Binds to fibrinogen beta promoter and is involved in IL6-induced fibrinogen beta transcriptional activation.
Phosphorylation on Thr-156 abolishes binding to target promoters and subsequent transcription activation upon insulin stimulation.
Nucleus. Cytoplasm.
Note: Shuttles between the nucleus and cytoplasm in a CRM1-dependent manner; in response to insulin signaling via AKT1 is exported from the nucleus.
Binds DNA as a monomer. Binds TLE1 (By similarity). Interacts with FOXA1 and FOXA3. Interacts with PRKDC.
研究領域
· Human Diseases > Endocrine and metabolic diseases > Maturity onset diabetes of the young.
· Organismal Systems > Aging > Longevity regulating pathway - multiple species. (View pathway)
参考文献
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