Myelin PLP Antibody - #DF13282

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製品: Myelin PLP Antibody
カタログ: DF13282
タンパク質の説明: Rabbit polyclonal antibody to Myelin PLP
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit, Dog
分子量: 30kDa(Observed); 30kD(Calculated).
ユニプロット: P60201
RRID: AB_2846301

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#DF13282-BP

Myelin PLP Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
IHC 1:50-1:200, WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
予測:
Pig(100%), Bovine(%), Sheep(%), Rabbit(%), Dog(%)
クローナリティ:
Polyclonal
特異性:
Myelin PLP Antibody detects endogenous levels of total Myelin PLP.
RRID:
AB_2846301
引用形式: Affinity Biosciences Cat# DF13282, RRID:AB_2846301.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

HLD1; Lipophilin; Major myelin proteolipid protein; MMPL; Myelin proteolipid protein; MYPR_HUMAN; PLP 1; PLP; PLP/DM20; PLP1; PMD; Proteolipid protein 1; SPG2;

免疫原

免疫原:

A synthesized peptide derived from human Myelin PLP, corresponding to a region within the internal amino acids.

Uniprot:

P60201(MYPR_HUMAN) >>Visit HPA database.

遺伝子(ID):
PLP1(5354)
タンパク質配列:
MGLLECCARCLVGAPFASLVATGLCFFGVALFCGCGHEALTGTEKLIETYFSKNYQDYEYLINVIHAFQYVIYGTASFFFLYGALLLAEGFYTTGAVRQIFGDYKTTICGKGLSATVTGGQKGRGSRGQHQAHSLERVCHCLGKWLGHPDKFVGITYALTVVWLLVFACSAVPVYIYFNTWTTCQSIAFPSKTSASIGSLCADARMYGVLPWNAFPGKVCGSNLLSICKTAEFQMTFHLFIAAFVGAAATLVSLLTFMIAATYNFAVLKLMGRGTKF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Chicken
50
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

This is the major myelin protein from the central nervous system. It plays an important role in the formation or maintenance of the multilamellar structure of myelin.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Myelin membrane.
Note: Colocalizes with SIRT2 in internodal regions, at paranodal axoglial junction and Schmidt-Lanterman incisures of myelin sheat.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the myelin proteolipid protein family.

参考文献

1). Proteomic analysis of the effects of caffeine in a neonatal rat model of hypoxic‐ischemic white matter damage. CNS Neuroscience & Therapeutics, 2022 (PubMed: 35393758) [IF=4.8]

Application: WB    Species: Rat    Sample: brain tissue

FIGURE 3 Caffeine improved myelin development after hypoxic‐ischemic WMD in neonatal rats. (A) Western blot detection of MBP, PLP, and MAG. Relative protein levels of (B) MBP, (C) PLP, and (D) MAG. (E) Immunohistochemical staining of MBP and LFB staining in the corpus callosum (CC) area of the ligated side cerebral hemisphere at 14 days after hypoxic‐ischemia (MBP scale bar = 50 μm; LBF scale bar = 50 μm). Average optical density (AOD) values of (F) MBP and (G) LBF staining. (H) Diagrammatic representation of the brain region analyzed for the level of MBP and LBF staining. Data are presented as means ± SEMs. Statistical analyses involved one‐way ANOVA, followed by Tukey's test. *p < 0.05, **p < 0.01, ***p < 0.001. Sham group (n = 6); HI group (n = 6); Caffeine group (n = 6); and Caffeine+AK‐7 group (n = 6). Ca, Caffeine group; Ca+AK‐7, Caffeine+AK‐7 group
2). Identification and preliminary analysis of hub genes associated with bladder cancer progression by comprehensive bioinformatics analysis. Scientific reports, 2024 (PubMed: 38307969) [IF=3.8]

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