製品: Nicotinic Acetylcholine Receptor alpha 7 Antibody
カタログ: DF13247
タンパク質の説明: Rabbit polyclonal antibody to Nicotinic Acetylcholine Receptor alpha 7
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Chicken
分子量: 56kDa, 48 kDa; 56kD(Calculated).
ユニプロット: P36544
RRID: AB_2846266

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Chicken(100%)
クローナリティ:
Polyclonal
特異性:
Nicotinic Acetylcholine Receptor alpha 7 Antibody detects endogenous levels of total Nicotinic Acetylcholine Receptor alpha 7.
RRID:
AB_2846266
引用形式: Affinity Biosciences Cat# DF13247, RRID:AB_2846266.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

7nAChR; a7 nicotinic acetylcholine receptor; acetylcholine receptor, neuronal nicotinic, alpha-7 subunit; ACHA7_HUMAN; AChR; Acra7; alpha 7 neuronal nicotinic acetylcholine receptor; alpha-7 nicotinic cholinergic receptor subunit; alpha7; cholinergic receptor, neuronal nicotinic, alpha polypeptide 7; cholinergic receptor, nicotinic, alpha 7 (neuronal); cholinergic receptor, nicotinic, alpha 7; cholinergic receptor, nicotinic, alpha polypeptide 7; CHRNA7; CHRNA7-2; NACHRA7; neuronal acetylcholine receptor protein, alpha-7 chain; Neuronal acetylcholine receptor subunit alpha-7;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MRCSPGGVWLALAASLLHVSLQGEFQRKLYKELVKNYNPLERPVANDSQPLTVYFSLSLLQIMDVDEKNQVLTTNIWLQMSWTDHYLQWNVSEYPGVKTVRFPDGQIWKPDILLYNSADERFDATFHTNVLVNSSGHCQYLPPGIFKSSCYIDVRWFPFDVQHCKLKFGSWSYGGWSLDLQMQEADISGYIPNGEWDLVGIPGKRSERFYECCKEPYPDVTFTVTMRRRTLYYGLNLLIPCVLISALALLVFLLPADSGEKISLGITVLLSLTVFMLLVAEIMPATSDSVPLIAQYFASTMIIVGLSVVVTVIVLQYHHHDPDGGKMPKWTRVILLNWCAWFLRMKRPGEDKVRPACQHKQRRCSLASVEMSAVAPPPASNGNLLYIGFRGLDGVHCVPTPDSGVVCGRMACSPTHDEHLLHGGQPPEGDPDLAKILEEVRYIANRFRCQDESEAVCSEWKFAACVVDRLCLMAFSVFTIICTIGILMSAPNFVEAVSKDFA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Chicken
100
Xenopus
75
Pig
0
Horse
0
Bovine
0
Sheep
0
Dog
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P36544 基板として

Site PTM Type Enzyme
Y30 Phosphorylation
T83 Phosphorylation
Y86 Phosphorylation
Y217 Phosphorylation
T221 Phosphorylation
T225 Phosphorylation
S365 Phosphorylation
Y386 Phosphorylation P12931 (SRC)
Y442 Phosphorylation P12931 (SRC)

研究背景

機能:

After binding acetylcholine, the AChR responds by an extensive change in conformation that affects all subunits and leads to opening of an ion-conducting channel across the plasma membrane. The channel is blocked by alpha-bungarotoxin.

細胞の位置付け:

Cell junction>Synapse>Postsynaptic cell membrane>Multi-pass membrane protein. Cell membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homopentamer (By similarity). Interacts with RIC3; which is required for proper folding and assembly. Interacts with LYPD6. Interacts with the alpha-conotoxin RgIA (By similarity). Interacts with alpha-conotoxins ImI and ImII.

タンパク質ファミリー:

Belongs to the ligand-gated ion channel (TC 1.A.9) family. Acetylcholine receptor (TC 1.A.9.1) subfamily. Alpha-7/CHRNA7 sub-subfamily.

研究領域

· Environmental Information Processing > Signal transduction > Calcium signaling pathway.   (View pathway)

· Environmental Information Processing > Signaling molecules and interaction > Neuroactive ligand-receptor interaction.

· Human Diseases > Cancers: Overview > Chemical carcinogenesis.

· Organismal Systems > Nervous system > Cholinergic synapse.

参考文献

1). α-Mangostin Treats Early-Stage Adjuvant-Induced Arthritis of Rat by Regulating the CAP-SIRT1 Pathway in Macrophages. Drug Design, Development and Therapy (PubMed: 35250263) [IF=4.8]

Application: WB    Species: Rat    Sample: RAW264.7 Cells

Figure 4 Effect of MG activated CAP-SIRT1 pathway on NF-κB-mediated inflammatory response in rat macrophages. (A) Expression of α7nAChR, SIRT1, ac-p65, p-p65 and p65 in macrophages investigated by immunoblotting; (B–E) quantitative result of (A). #P<0.05, ##P<0.01, ###P<0.001 compared with normal, *P<0.05, **P<0.01, ***P<0.001 compared with AIA rats, &P<0.05 compared with MG (early).

2). The prenatal hypoxic pathology associated with maternal stress predisposes to dysregulated expression of the chrna7 gene and the subsequent development of nicotine addiction in adult offspring. Neuroendocrinology (PubMed: 38198758) [IF=4.1]

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