Kv4.3 Antibody - #DF13239

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製品: Kv4.3 Antibody
カタログ: DF13239
タンパク質の説明: Rabbit polyclonal antibody to Kv4.3
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Bovine, Horse, Sheep, Rabbit, Chicken, Xenopus
分子量: 73kDa(Observed); 73kD(Calculated).
ユニプロット: Q9UK17
RRID: AB_2846258

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(88%), Horse(88%), Sheep(88%), Rabbit(100%), Chicken(100%), Xenopus(88%)
クローナリティ:
Polyclonal
特異性:
Kv4.3 Antibody detects endogenous levels of total Kv4.3.
RRID:
AB_2846258
引用形式: Affinity Biosciences Cat# DF13239, RRID:AB_2846258.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

KCND 3; KCND 3L; KCND 3S; Kcnd3; KCND3_HUMAN; KCND3L; KCND3S; KSHIVB; Kv 4.3; MGC14203; MGC142037; OTTHUMP00000063948; Potassium ioinic channel Kv4.3; Potassium voltage gated channel Shal related subfamily member 3; Potassium voltage gated channel subfamily D member 3; Potassium voltage-gated channel subfamily D member 3; Sha1 related potassium channel Kv4.3; Voltage gated K+ channel; Voltage gated potassium channel Kv4.3; Voltage gated potassium channel subunit Kv4.3; Voltage-gated potassium channel subunit Kv4.3;

免疫原

免疫原:

A synthesized peptide derived from human Kv4.3, corresponding to a region within the internal amino acids.

Uniprot:

Q9UK17(KCND3_HUMAN) >>Visit HPA database.

遺伝子(ID):
KCND3(3752)
発現特異性:
Q9UK17 KCND3_HUMAN:

Highly expressed in heart and brain, in particular in cortex, cerebellum, amygdala and caudate nucleus. Detected at lower levels in liver, skeletal muscle, kidney and pancreas. Isoform 1 predominates in most tissues. Isoform 1 and isoform 2 are detected at similar levels in brain, skeletal muscle and pancreas.

タンパク質配列:
MAAGVAAWLPFARAAAIGWMPVANCPMPLAPADKNKRQDELIVLNVSGRRFQTWRTTLERYPDTLLGSTEKEFFFNEDTKEYFFDRDPEVFRCVLNFYRTGKLHYPRYECISAYDDELAFYGILPEIIGDCCYEEYKDRKRENAERLMDDNDSENNQESMPSLSFRQTMWRAFENPHTSTLALVFYYVTGFFIAVSVITNVVETVPCGTVPGSKELPCGERYSVAFFCLDTACVMIFTVEYLLRLFAAPSRYRFIRSVMSIIDVVAIMPYYIGLVMTNNEDVSGAFVTLRVFRVFRIFKFSRHSQGLRILGYTLKSCASELGFLLFSLTMAIIIFATVMFYAEKGSSASKFTSIPASFWYTIVTMTTLGYGDMVPKTIAGKIFGSICSLSGVLVIALPVPVIVSNFSRIYHQNQRADKRRAQKKARLARIRVAKTGSSNAYLHSKRNGLLNEALELTGTPEEEHMGKTTSLIESQHHHLLHCLEKTTGLSYLVDDPLLSVRTSTIKNHEFIDEQMFEQNCMESSMQNYPSTRSPSLSSHPGLTTTCCSRRSKKTTHLPNSNLPATRLRSMQELSTIHIQGSEQPSLTTSRSSLNLKADDGLRPNCKTSQITTAIISIPTPPALTPEGESRPPPASPGPNTNIPSIASNVVKVSAL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Chicken
100
Horse
88
Bovine
88
Sheep
88
Xenopus
88
Pig
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Pore-forming (alpha) subunit of voltage-gated rapidly inactivating A-type potassium channels. May contribute to I(To) current in heart and I(Sa) current in neurons. Channel properties are modulated by interactions with other alpha subunits and with regulatory subunits.

PTMs:

Regulated through phosphorylation at Ser-569 by CaMK2D.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Cell membrane>Sarcolemma>Multi-pass membrane protein. Cell projection>Dendrite.
Note: Interaction with palmitoylated KCNIP2 and KCNIP3 enhances cell surface expression.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highly expressed in heart and brain, in particular in cortex, cerebellum, amygdala and caudate nucleus. Detected at lower levels in liver, skeletal muscle, kidney and pancreas. Isoform 1 predominates in most tissues. Isoform 1 and isoform 2 are detected at similar levels in brain, skeletal muscle and pancreas.

タンパク質ファミリー:

The segment S4 is probably the voltage-sensor and is characterized by a series of positively charged amino acids at every third position.

Belongs to the potassium channel family. D (Shal) (TC 1.A.1.2) subfamily. Kv4.3/KCND3 sub-subfamily.

参考文献

1). Rare KCND3 Loss-of-Function Mutation Associated With the SCA19/22. INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES, 2022 (PubMed: 34067185) [IF=5.6]
2). Role of Ion Channels in Chronic Intermittent Hypoxia-Induced Atrial Remodeling in Rats. LIFE SCIENCES, 2020 (PubMed: 32417371) [IF=5.2]

Application: WB    Species: rat    Sample: atrial

Fig. 6.| Protein levels of Nav1.5, Cav1.2, Kv4.2, Kv4.3, Kv1.5, NCX1, RyR2, p-RyR2, CaMKII, p-CaMKII confirmed by Western Blotting in two groups (n = 10). And p-RyR2/RyR2, p-CaMKII/CaMKII were also calculated and compared between two groups. LA -left atrial, RA -right atrial.

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