Stra8 Antibody - #DF13234

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製品: Stra8 Antibody
カタログ: DF13234
タンパク質の説明: Rabbit polyclonal antibody to Stra8
アプリケーション: WB IHC IF/ICC
Cited expt.: WB, IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 36-60kD(Observed); 37kD(Calculated).
ユニプロット: Q7Z7C7
RRID: AB_2846253

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#DF13234-BP

Stra8 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human, Mouse, Rat
予測:
Pig(100%), Bovine(%), Horse(%), Sheep(%), Dog(%)
クローナリティ:
Polyclonal
特異性:
Stra8 Antibody detects endogenous levels of total Stra8.
RRID:
AB_2846253
引用形式: Affinity Biosciences Cat# DF13234, RRID:AB_2846253.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

Stimulated by retinoic acid 8; stimulated by retinoic acid 8 homolog; Stimulated by retinoic acid Gene 8; stimulated by retinoic acid gene 8 homolog; Stimulated by retinoic acid gene 8 protein homolog; STRA 8; STRA8; STRA8_HUMAN;

免疫原

免疫原:

A synthesized peptide derived from human Stra8, corresponding to a region within the internal amino acids.

Uniprot:

Q7Z7C7(STRA8_HUMAN) >>Visit HPA database.

遺伝子(ID):
STRA8(346673)
発現特異性:
Q7Z7C7 STRA8_HUMAN:

Expressed specifically in testis and fetal ovaries.

タンパク質配列:
MGKIDVDKILFFNQEIRLWQLIMATPEENSNPHDRATPQLPAQLQELEHRVARRRLSQARHRATLAALFNNLRKTVYSQSDLIASKWQVLNKAKSHIPELEQTLDNLLKLKASFNLEDGHASSLEEVKKEYASMYSGNDSFPQNGSSPWYLNFYKQTMDLLTGSGIITPQEAALPIVSAAISHLWQNLSEERKASLRQAWAQKHRGPATLAEACREPACAEGSVKDSGVDSQGASCSLVSTPEEILFEDAFDVASFLDKSEVPSTSSSSSVLASCNPENPEEKFQLYMQIINFFKGLSCANTQVKQEASFPVDEEMIMLQCTETFDDEDL

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Meiosis-inducer required for the transition into meiosis for both female and male germ cells. In female germ cells, required for premeiotic DNA replication and subsequent events in meiotic prophase. During spermatogenesis, next to its role in meiotic initiation, promotes (but is not required for) spermatogonial differentiation. Can associate with DNA (possibly in an indirect manner), and in vitro can activate DNA transcription (By similarity).

PTMs:

Phosphorylated.

細胞の位置付け:

Cytoplasm. Nucleus.
Note: Shuttles between nucleus and cytoplasm. Nuclear export is XPO1-dependent.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed specifically in testis and fetal ovaries.

参考文献

1). Long noncoding RNAs regulated spermatogenesis in varicocele‐induced spermatogenic dysfunction. CELL PROLIFERATION, 2022 (PubMed: 35297519) [IF=5.9]

Application: WB    Species: rat    Sample: Testicular

FIGURE 13| Validation of regulated signalling pathways, spermatogenic cell apoptosis and proliferation, and meiotic spermatocytes by Western blot. Representative Western blot images of PI3K, Akt, p-Akt, caspase-9, Bcl-2, Bax, PCNA, PLZF, REC8, STRA8, and SYCP3.
2). Differential Impairment Mechanism of Sperm Production via Induction of miR-34c-Activated Apoptosis and Spermatogenesis Pathway in Diet-Induced Obesity and Resistant Mice and GC-1 Spg Cells. International journal of molecular sciences, 2024 (PubMed: 39000558) [IF=5.6]

Application: WB    Species: Mouse    Sample: testicular tissues

Figure 4 The effect of a high-fat diet on miR-34c-5p and spermatogenesis-related proteins in testicular tissues among DIO-R, DIO, and control mice. (a) miR-34c-5p levels in testicular tissue (n = 6); (b) representative Western blots using antibodies against RARg, Stra8, and PLZF in testicular tissue (n = 6). (c–e) The bar graphs show the results of the semi-quantitative measurement of RARg, Stra8, and PLZF expression, respectively. All data are expressed as mean ± SD; * p < 0.05, ** p < 0.01, versus control group; # p < 0.05, ## p < 0.01, versus DIO group.
3). Mouse Pramel1 regulates spermatogonial development by inhibiting retinoic acid signaling during spermatogenesis. Development (Cambridge, England), 2023 (PubMed: 37781892) [IF=3.7]

Application: IF/ICC    Species: Mouse    Sample:

Fig. 3. Spermatogonial population dynamics during neonatal development are altered in the absence of PRAMEL1. (A) Immunofluorescent staining with PLZF (green) and STRA8 (red) antibodies, counterstained with DAPI (blue), on testis cross-sections at P2-P6. Arrows indicate the PLZF+/STRA8+ double-positive cells. Negative controls are in the right top corners of images. (B) Number of STRA8+/PLZF+ double-positive spermatogonia/mm2 and of PLZF+ cells/mm2. (C) Relative RNA expression level (wild type is set as 1, dashed line) in spermatogonia. (D) Flow cytometry analysis of ID4-EGFPBright, ID4-EGFPMid, ID4-EGFPDim and ID4-EGFP− among DDX4+ cells. The immunofluorescent staining experiments in A, qRT-PCR in C and flow cytometry analysis in D were repeated three times. Data are mean±s.e.m., n=4 or 5; *P

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