製品: Phospho-ATRIP (Ser68) Antibody
カタログ: AF3468
タンパク質の説明: Rabbit polyclonal antibody to Phospho-ATRIP (Ser68)
アプリケーション: WB IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Sheep, Rabbit, Dog, Chicken
分子量: 80kDa; 86kD(Calculated).
ユニプロット: Q8WXE1
RRID: AB_2834906

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Sheep(100%), Rabbit(89%), Dog(100%), Chicken(89%)
クローナリティ:
Polyclonal
特異性:
Phospho-ATRIP (Ser68) Antibody detects endogenous levels of ATRIP only when phosphorylated at Serine 68.
RRID:
AB_2834906
引用形式: Affinity Biosciences Cat# AF3468, RRID:AB_2834906.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

AGS1; ATIP; ATM and Rad3 related interacting protein; ATM and Rad3-related-interacting protein; ATR interacting protein; ATR-interacting protein; Atrip; ATRIP_HUMAN; DKFZp762J2115; FLJ12343; MGC20625; MGC21482; MGC26740;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q8WXE1 ATRIP_HUMAN:

Ubiquitous.

タンパク質の説明:
ATRIP is required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.
タンパク質配列:
MAGTSAPGSKRRSEPPAPRPGPPPGTGHPPSKRARGFSAAAAPDPDDPFGAHGDFTADDLEELDTLASQALSQCPAAARDVSSDHKVHRLLDGMSKNPSGKNRETVPIKDNFELEVLQAQYKELKEKMKVMEEEVLIKNGEIKILRDSLHQTESVLEEQRRSHFLLEQEKTQALSDKEKEFSKKLQSLQSELQFKDAEMNELRTKLQTSERANKLAAPSVSHVSPRKNPSVVIKPEACSPQFGKTSFPTKESFSANMSLPHPCQTESGYKPLVGREDSKPHSLRGDSIKQEEAQKSFVDSWRQRSNTQGSILINLLLKQPLIPGSSLSLCHLLSSSSESPAGTPLQPPGFGSTLAGMSGLRTTGSYDGSFSLSALREAQNLAFTGLNLVARNECSRDGDPAEGGRRAFPLCQLPGAVHFLPLVQFFIGLHCQALQDLAAAKRSGAPGDSPTHSSCVSSGVETNPEDSVCILEGFSVTALSILQHLVCHSGAVVSLLLSGVGADSAAGEGNRSLVHRLSDGDMTSALRGVADDQGQHPLLKMLLHLLAFSSAATGHLQASVLTQCLKVLVKLAENTSCDFLPRFQCVFQVLPKCLSPETPLPSVLLAVELLSLLADHDQLAPQLCSHSEGCLLLLLYMYITSRPDRVALETQWLQLEQEVVWLLAKLGVQSPLPPVTGSNCQCNVEVVRALTVMLHRQWLTVRRAGGPPRTDQQRRTVRCLRDTVLLLHGLSQKDKLFMMHCVEVLHQFDQVMPGVSMLIRGLPDVTDCEEAALDDLCAAETDVEDPEVECG

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Sheep
100
Dog
100
Chicken
89
Rabbit
89
Horse
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q8WXE1 基板として

Site PTM Type Enzyme
S9 Phosphorylation
S13 Phosphorylation
T26 Phosphorylation
S31 Phosphorylation
K32 Acetylation
S38 Phosphorylation
S68 Phosphorylation Q13535 (ATR)
S72 Phosphorylation Q13535 (ATR)
K96 Ubiquitination
K122 Ubiquitination
S162 Phosphorylation
K205 Ubiquitination
K214 Ubiquitination
S219 Phosphorylation
S221 Phosphorylation
S224 Phosphorylation P24941 (CDK2)
S239 Phosphorylation P24941 (CDK2)
K270 Ubiquitination
K295 Ubiquitination
S305 Phosphorylation
T307 Phosphorylation
S310 Phosphorylation
S512 Phosphorylation
S518 Phosphorylation
T523 Phosphorylation
Y636 Phosphorylation

研究背景

機能:

Required for checkpoint signaling after DNA damage. Required for ATR expression, possibly by stabilizing the protein.

PTMs:

Phosphorylated by ATR.

細胞の位置付け:

Nucleus.
Note: Redistributes to discrete nuclear foci upon DNA damage.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous.

サブユニット構造:

Interacts with ATR (By similarity). Heterodimer with ATR. The heterodimer binds the RPA complex and is then recruited to single-stranded DNA. Interacts with CEP164 (via N-terminus). Interacts with CINP.

タンパク質ファミリー:

The EEXXXDDL motif is required for the interaction with catalytic subunit PRKDC and its recruitment to sites of DNA damage.

Belongs to the ATRIP family.

研究領域

· Genetic Information Processing > Replication and repair > Fanconi anemia pathway.

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