製品: ODAM Antibody
カタログ: DF13204
タンパク質の説明: Rabbit polyclonal antibody to ODAM
アプリケーション: WB IHC
Cited expt.:
反応性: Human, Mouse
予測: Pig, Horse, Rabbit, Dog
分子量: 17 kDa(Observed); 31kD(Calculated).
ユニプロット: A1E959
RRID: AB_2846164

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Horse(90%), Rabbit(80%), Dog(90%)
クローナリティ:
Polyclonal
特異性:
ODAM Antibody detects endogenous levels of total ODAM.
RRID:
AB_2846164
引用形式: Affinity Biosciences Cat# DF13204, RRID:AB_2846164.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.

免疫原

免疫原:

A synthesized peptide derived from human ODAM, corresponding to a region within N-terminal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
A1E959 ODAM_HUMAN:

Expressed in the junctional epithelium of healthy teeth. In periodontitis, absent in the pocket epithelium of the diseased periodontium but is detected in the gingival crevicular fluid.

タンパク質配列:
MKIIILLGFLGATLSAPLIPQRLMSASNSNELLLNLNNGQLLPLQLQGPLNSWIPPFSGILQQQQQAQIPGLSQFSLSALDQFAGLLPNQIPLTGEASFAQGAQAGQVDPLQLQTPPQTQPGPSHVMPYVFSFKMPQEQGQMFQYYPVYMVLPWEQPQQTVPRSPQQTRQQQYEEQIPFYAQFGYIPQLAEPAISGGQQQLAFDPQLGTAPEIAVMSTGEEIPYLQKEAINFRHDSAGVFMPSTSPKPSTTNVFTSAVDQTITPELPEEKDKTDSLREP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
90
Dog
90
Rabbit
80
Bovine
78
Sheep
67
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Tooth-associated epithelia protein that probably plays a role in odontogenesis, the complex process that results in the initiation and generation of the tooth. May be incorporated in the enamel matrix at the end of mineralization process. Involved in the induction of RHOA activity via interaction with ARHGEF and expression of downstream factors such as ROCK. Plays a role in attachment of the junctional epithelium to the tooth surface.

PTMs:

O-glycosylated.

細胞の位置付け:

Secreted. Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in the junctional epithelium of healthy teeth. In periodontitis, absent in the pocket epithelium of the diseased periodontium but is detected in the gingival crevicular fluid.

タンパク質ファミリー:

Belongs to the ODAM family.

参考文献

1). Single-cell transcriptomics reveals cell atlas and identifies cycling tumor cells responsible for recurrence in ameloblastoma. International journal of oral science, 2024 (PubMed: 38424060) [IF=10.8]

Application: IF/ICC    Species: Human    Sample:

Single-cell transcriptomic profiling of the multicellular ecosystem of ameloblastoma. a Workflow showing the collection and processing of fresh patient samples from 11 tumors for scRNA-seq. (b) UMAP plot of 92,622 single cells from scRNA-seq labeled by cell type with different colors. c UMAP plot showing the expression of selected marker genes in the 9 clusters. (d) Representative images of multiplex immunohistochemistry showing the expression of PCK, CD3, CD34, CD68, ODAM, FAP in ameloblastoma; scale bar, 100 μm, and 20 μm

2). Targeting BRD4 attenuates the stemness and aggressiveness of ameloblastoma. Oral diseases, 2024 (PubMed: 37798926) [IF=2.9]

3). A novel junctional epithelial cell line, mHAT-JE01, derived from incisor epithelial cells. Journal of Oral Biosciences, 2023 (PubMed: 36693475) [IF=2.6]

4). Odontogenic ameloblast-associated protein (ODAM)-enriched coating improves adhesion of junctional epithelial cells to composite resin via AKT activation. Journal of oral biosciences, 2026 (PubMed: 41714024) [IF=2.6]

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