製品: NIT2 Antibody
カタログ: DF13186
タンパク質の説明: Rabbit polyclonal antibody to NIT2
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 30 kDa; 31kD(Calculated).
ユニプロット: Q9NQR4
RRID: AB_2846146

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(91%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(91%)
クローナリティ:
Polyclonal
特異性:
NIT2 Antibody detects endogenous levels of total NIT2.
RRID:
AB_2846146
引用形式: Affinity Biosciences Cat# DF13186, RRID:AB_2846146.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

CUA002; MGC111199; NIT 2; Nit protein 2; NIT2; NIT2_HUMAN; Nitrilase family member 2; Nitrilase homolog 2; Omega amidase NIT2; Omega-amidase NIT2;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q9NQR4 NIT2_HUMAN:

Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.

タンパク質配列:
MTSFRLALIQLQISSIKSDNVTRACSFIREAATQGAKIVSLPECFNSPYGAKYFPEYAEKIPGESTQKLSEVAKECSIYLIGGSIPEEDAGKLYNTCAVFGPDGTLLAKYRKIHLFDIDVPGKITFQESKTLSPGDSFSTFDTPYCRVGLGICYDMRFAELAQIYAQRGCQLLVYPGAFNLTTGPAHWELLQRSRAVDNQVYVATASPARDDKASYVAWGHSTVVNPWGEVLAKAGTEEAIVYSDIDLKKLAEIRQQIPVFRQKRSDLYAVEMKKP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Sheep
100
Rabbit
100
Bovine
91
Dog
91
Zebrafish
64
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9NQR4 基板として

Site PTM Type Enzyme
S26 Phosphorylation
K37 Ubiquitination
Y49 Phosphorylation
K52 Ubiquitination
K60 Ubiquitination
K68 Acetylation
K68 Ubiquitination
K92 Ubiquitination
K109 Ubiquitination
K112 Ubiquitination
K130 Ubiquitination
S133 Phosphorylation
Y145 Phosphorylation
C146 S-Nitrosylation
Y165 Phosphorylation
Y202 Phosphorylation
S207 Phosphorylation
T237 Phosphorylation
S244 Phosphorylation
K249 Acetylation
K249 Ubiquitination
K250 Acetylation
R265 Methylation
S266 Phosphorylation
Y269 Phosphorylation

研究背景

機能:

Has a omega-amidase activity. The role of omega-amidase is to remove potentially toxic intermediates by converting alpha-ketoglutaramate and alpha-ketosuccinamate to biologically useful alpha-ketoglutarate and oxaloacetate, respectively. Overexpression decreases the colony-forming capacity of cultured cells by arresting cells in the G2 phase of the cell cycle.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Detected in fetal brain (at protein level). Ubiquitous. Detected in heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, prostate, spleen, thymus, prostate, testis, ovary, small intestine and colon.

サブユニット構造:

Homodimer.

タンパク質ファミリー:

Belongs to the carbon-nitrogen hydrolase superfamily. NIT1/NIT2 family.

研究領域

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

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