HIST1H1T Antibody - #DF13061

(8)  
製品: HIST1H1T Antibody
カタログ: DF13061
タンパク質の説明: Rabbit polyclonal antibody to HIST1H1T
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse
分子量: 30 kDa; 22kD(Calculated).
ユニプロット: P22492
RRID: AB_2846022

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
クローナリティ:
Polyclonal
特異性:
HIST1H1T Antibody detects endogenous levels of total HIST1H1T.
RRID:
AB_2846022
引用形式: Affinity Biosciences Cat# DF13061, RRID:AB_2846022.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

H1 histone family, member T (testis specific); H1FT; H1T; H1T_HUMAN; Hist1h1t; Histone cluster 1, H1t; Histone H1t; Testicular H1 histone;

免疫原

免疫原:

A synthesized peptide derived from human HIST1H1T, corresponding to a region within the internal amino acids.

Uniprot:

P22492(H1T_HUMAN) >>Visit HPA database.

遺伝子(ID):
HIST1H1T(3010)
発現特異性:
P22492 H1T_HUMAN:

Testis-specific.

タンパク質配列:
MSETVPAASASAGVAAMEKLPTKKRGRKPAGLISASRKVPNLSVSKLITEALSVSQERVGMSLVALKKALAAAGYDVEKNNSRIKLSLKSLVNKGILVQTRGTGASGSFKLSKKVIPKSTRSKAKKSVSAKTKKLVLSRDSKSPKTAKTNKRAKKPRATTPKTVRSGRKAKGAKGKQQQKSPVKARASKSKLTQHHEVNVRKATSKK

研究背景

機能:

Testis-specific histone H1 that forms less compacted chromatin compared to other H1 histone subtypes. Formation of more relaxed chromatin may be required to promote chromatin architecture required for proper chromosome regulation during meiosis, such as homologous recombination. Histones H1 act as linkers that bind to nucleosomes and compact polynucleosomes into a higher-order chromatin configuration (Probable).

PTMs:

Phosphorylated in early spermatids.

Citrullination at Arg-58 (H1R54ci) by PADI4 takes place within the DNA-binding site of H1 and results in its displacement from chromatin and global chromatin decondensation, thereby promoting pluripotency and stem cell maintenance.

細胞の位置付け:

Nucleus. Chromosome.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Testis-specific.

タンパク質ファミリー:

Belongs to the histone H1/H5 family.

Restrictive clause

 

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