FAM134B Antibody - #DF12997

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製品: FAM134B Antibody
カタログ: DF12997
タンパク質の説明: Rabbit polyclonal antibody to FAM134B
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 65 kDa; 55kD(Calculated).
ユニプロット: Q9H6L5
RRID: AB_2845958

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 100ul $280 在庫あり
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
FAM134B Antibody detects endogenous levels of total FAM134B.
RRID:
AB_2845958
引用形式: Affinity Biosciences Cat# DF12997, RRID:AB_2845958.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

F134B_HUMAN; FAM134B; family with sequence similarity 134 member B; HSAN2B; JK 1; JK1; Protein FAM134B;

免疫原

免疫原:

A synthesized peptide derived from human FAM134B, corresponding to a region within the internal amino acids.

Uniprot:

Q9H6L5(RETR1_HUMAN) >>Visit HPA database.

遺伝子(ID):
RETREG1(54463)
発現特異性:
Q9H6L5 RETR1_HUMAN:

Overexpressed in esophageal squamous cell carcinoma (PubMed:17487424).

タンパク質配列:
MASPAPPEHAEEGCPAPAAEEQAPPSPPPPQASPAERQQQEEEAQEAGAAEGAGLQVEEAAGRAAAAVTWLLGEPVLWLGCRADELLSWKRPLRSLLGFVAANLLFWFLALTPWRVYHLISVMILGRVIMQIIKDMVLSRTRGAQLWRSLSESWEVINSKPDERPRLSHCIAESWMNFSIFLQEMSLFKQQSPGKFCLLVCSVCTFFTILGSYIPGVILSYLLLLCAFLCPLFKCNDIGQKIYSKIKSVLLKLDFGIGEYINQKKRERSEADKEKSHKDDSELDFSALCPKISLTVAAKELSVSDTDVSEVSWTDNGTFNLSEGYTPQTDTSDDLDRPSEEVFSRDLSDFPSLENGMGTNDEDELSLGLPTELKRKKEQLDSGHRPSKETQSAAGLTLPLNSDQTFHLMSNLAGDVITAAVTAAIKDQLEGVQQALSQAAPIPEEDTDTEEGDDFELLDQSELDQIESELGLTQDQEAEAQQNKKSSGFLSNLLGGH

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Xenopus
100
Rabbit
100
Zebrafish
75
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Endoplasmic reticulum-anchored autophagy receptor that mediates ER delivery into lysosomes through sequestration into autophagosomes. Promotes membrane remodeling and ER scission via its membrane bending capacity and targets the fragments into autophagosomes via interaction with ATG8 family proteins. Required for long-term survival of nociceptive and autonomic ganglion neurons.

細胞の位置付け:

Golgi apparatus>cis-Golgi network membrane>Multi-pass membrane protein. Endoplasmic reticulum membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Overexpressed in esophageal squamous cell carcinoma.

タンパク質ファミリー:

The LIR motif interacts with ATG8 family proteins and is necessary to target the ER fragments to autophagosomes for subsequent lysosomal degradation.

The reticulon homology domain provides capacity to bend the membrane and promotes ER scission (PubMed:26040720). This domain does not show relevant similarities with reticulon domains, preventing any domain predictions within the protein sequence.

Belongs to the RETREG family.

参考文献

1). SARS-CoV-2 ORF3a induces RETREG1/FAM134B-dependent reticulophagy and triggers sequential ER stress and inflammatory responses during SARS-CoV-2 infection. Autophagy, 2022 (PubMed: 35239449) [IF=14.6]
2). Physical exercise mitigates the cognitive impairments by promoting ER-phagy in mice model of Alzheimer's disease. Behavioural brain research, 2025 (PubMed: 40414310) [IF=2.6]
3). Investigation of the biomarkers and mechanism based on endoplasmic reticulum stress in irritable bowel syndrome. Arab journal of gastroenterology : the official publication of the Pan-Arab Association of Gastroenterology, 2025 (PubMed: 40987697) [IF=1.1]
4). Sequential ER stress and inflammatory responses are induced by SARS-CoV-2 ORF3 through ERphagy. bioRxiv, 2020

Application: WB    Species: Human    Sample: HEK293T cells

Figure S2.ORF3 does not affect FAM134B expression Flag-ORF3 or empty vector was transfected into HEK293T cells. Thirty-six hours after transfection, cell pellets were collected, and the whole cell extracts were analyzed by Western blots to detect the level of FAM134B expression.

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