製品: BCAT2 Antibody
カタログ: DF12851
タンパク質の説明: Rabbit polyclonal antibody to BCAT2
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Bovine
分子量: 42 kDa; 44kD(Calculated).
ユニプロット: O15382
RRID: AB_2845812

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(85%)
クローナリティ:
Polyclonal
特異性:
BCAT2 Antibody detects endogenous levels of total BCAT2.
RRID:
AB_2845812
引用形式: Affinity Biosciences Cat# DF12851, RRID:AB_2845812.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

BCAM; BCAT 2; BCAT(m); BCAT2; BCAT2_HUMAN; BCATM; BCT2; Branched chain aminotransferase 2, mitochondrial; Branched-chain-amino-acid aminotransferase; ECA40; mitochondrial; Placental protein 18; PP18;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
O15382 BCAT2_HUMAN:

Ubiquitous.

タンパク質配列:
MAAAALGQIWARKLLSVPWLLCGPRRYASSSFKAADLQLEMTQKPHKKPGPGEPLVFGKTFTDHMLMVEWNDKGWGQPRIQPFQNLTLHPASSSLHYSLQLFEGMKAFKGKDQQVRLFRPWLNMDRMLRSAMRLCLPSFDKLELLECIRRLIEVDKDWVPDAAGTSLYVRPVLIGNEPSLGVSQPTRALLFVILCPVGAYFPGGSVTPVSLLADPAFIRAWVGGVGNYKLGGNYGPTVLVQQEALKRGCEQVLWLYGPDHQLTEVGTMNIFVYWTHEDGVLELVTPPLNGVILPGVVRQSLLDMAQTWGEFRVVERTITMKQLLRALEEGRVREVFGSGTACQVCPVHRILYKDRNLHIPTMENGPELILRFQKELKEIQYGIRAHEWMFPV

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
85
Horse
77
Sheep
77
Xenopus
77
Pig
0
Dog
0
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - O15382 基板として

Site PTM Type Enzyme
K44 Acetylation
K44 Methylation
K73 Acetylation
C135 S-Nitrosylation
K141 Acetylation
Y228 Phosphorylation
K229 Acetylation
K229 Ubiquitination
K246 Acetylation
K246 Ubiquitination
K321 Acetylation
K321 Ubiquitination
C342 S-Nitrosylation
C345 S-Nitrosylation
K353 Acetylation
K353 Ubiquitination
K374 Ubiquitination
K377 Acetylation
K377 Ubiquitination

研究背景

機能:

Catalyzes the first reaction in the catabolism of the essential branched chain amino acids leucine, isoleucine, and valine. May also function as a transporter of branched chain alpha-keto acids.

細胞の位置付け:

Mitochondrion.

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous.

サブユニット構造:

Homodimer.

タンパク質ファミリー:

Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family.

研究領域

· Metabolism > Amino acid metabolism > Cysteine and methionine metabolism.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine degradation.

· Metabolism > Amino acid metabolism > Valine, leucine and isoleucine biosynthesis.

· Metabolism > Metabolism of cofactors and vitamins > Pantothenate and CoA biosynthesis.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > 2-Oxocarboxylic acid metabolism.

· Metabolism > Global and overview maps > Biosynthesis of amino acids.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
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