SH3BP5 Antibody - #DF12738
| 製品: | SH3BP5 Antibody |
| カタログ: | DF12738 |
| タンパク質の説明: | Rabbit polyclonal antibody to SH3BP5 |
| アプリケーション: | WB IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Bovine, Horse, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 47 kDa; 50kD(Calculated). |
| ユニプロット: | O60239 |
| RRID: | AB_2845699 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12738, RRID:AB_2845699.
折りたたみ／展開
3BP5_HUMAN; OTTHUMP00000208289; OTTHUMP00000208291; OTTHUMP00000208294; SAB; SH3 binding protein; SH3 domain binding protein 5 (BTK associated); SH3 domain binding protein 5; SH3 domain binding protein that preferentially associates with BTK; SH3 domain-binding protein 5; SH3 domain-binding protein that preferentially associates with BTK; SH3 domainbinding protein 5; SH3BP 5; SH3BP-5; Sh3bp5;
免疫原
Highly expressed in testis and ovaries. It is also expressed in a variety of tissues including spleen, lymph node, thymus, bone marrow, fetal liver, colon, small intestine and prostate.
- O60239 3BP5_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MDAALKRSRSEEPAEILPPARDEEEEEEEGMEQGLEEEEEVDPRIQGELEKLNQSTDDINRRETELEDARQKFRSVLVEATVKLDELVKKIGKAVEDSKPYWEARRVARQAQLEAQKATQDFQRATEVLRAAKETISLAEQRLLEDDKRQFDSAWQEMLNHATQRVMEAEQTKTRSELVHKETAARYNAAMGRMRQLEKKLKRAINKSKPYFELKAKYYVQLEQLKKTVDDLQAKLTLAKGEYKMALKNLEMISDEIHERRRSSAMGPRGCGVGAEGSSTSVEDLPGSKPEPDAISVASEAFEDDSCSNFVSEDDSETQSVSSFSSGPTSPSEMPDQFPAVVRPGSLDLPSPVSLSEFGMMFPVLGPRSECSGASSPECEVERGDRAEGAENKTSDKANNNRGLSSSSGSGGSSKSQSSTSPEGQALENRMKQLSLQCSKGRDGIIADIKMVQIG
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O60239 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| K6 | Ubiquitination | Uniprot | |
| S10 | Phosphorylation | Uniprot | |
| K51 | Ubiquitination | Uniprot | |
| S55 | Phosphorylation | Uniprot | |
| K93 | Ubiquitination | Uniprot | |
| K99 | Ubiquitination | Uniprot | |
| Y101 | Phosphorylation | Uniprot | |
| K117 | Ubiquitination | Uniprot | |
| K133 | Ubiquitination | Uniprot | |
| T135 | Phosphorylation | Uniprot | |
| S137 | Phosphorylation | Uniprot | |
| K173 | Ubiquitination | Uniprot | |
| K181 | Ubiquitination | Uniprot | |
| S208 | Phosphorylation | Uniprot | |
| K209 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K217 | Ubiquitination | Uniprot | |
| K226 | Ubiquitination | Uniprot | |
| K227 | Ubiquitination | Uniprot | |
| K235 | Acetylation | Uniprot | |
| K235 | Methylation | Uniprot | |
| K235 | Ubiquitination | Uniprot | |
| K240 | Methylation | Uniprot | |
| K240 | Ubiquitination | Uniprot | |
| K244 | Ubiquitination | Uniprot | |
| K248 | Ubiquitination | Uniprot | |
| S351 | Phosphorylation | P53778 (MAPK12) , P45984 (MAPK9) | Uniprot |
| S375 | Phosphorylation | Uniprot | |
| S376 | Phosphorylation | Uniprot | |
| K415 | Ubiquitination | Uniprot | |
| S416 | Phosphorylation | Uniprot | |
| S421 | Phosphorylation | Uniprot | |
| K432 | Ubiquitination | Uniprot | |
| S439 | Phosphorylation | Uniprot | |
| K440 | Ubiquitination | Uniprot |
研究背景
Functions as guanine nucleotide exchange factor (GEF) with specificity for RAB11A and RAB25. Inhibits the auto- and transphosphorylation activity of BTK. Plays a negative regulatory role in BTK-related cytoplasmic signaling in B-cells. May be involved in BCR-induced apoptotic cell death.
Cytoplasmic vesicle membrane>Peripheral membrane protein. Mitochondrion.
Note: Colocalizes with RAB11A on cytoplasmic vesicle membranes.
Highly expressed in testis and ovaries. It is also expressed in a variety of tissues including spleen, lymph node, thymus, bone marrow, fetal liver, colon, small intestine and prostate.
Interacts with BTK. Interacts with all isoforms of MAPK8, MAPK9, MAPK10 and MAPK12. Interacts with GDP-bound and nucleotide-free forms of RAB11A.
The N-terminal half of the protein mediates interaction with RAB11A and functions as guanine nucleotide exchange factor. Four long alpha-helices (interrupted by a central kink) assemble into coiled coils, giving rise to a 'V' shape.
Belongs to the SH3BP5 family.
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