TM158 Antibody - #DF12531

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製品: TM158 Antibody
カタログ: DF12531
タンパク質の説明: Rabbit polyclonal antibody to TM158
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human, Mouse
予測: Pig, Bovine, Chicken
分子量: 30 kDa; 30kD(Calculated).
ユニプロット: Q8WZ71
RRID: AB_2845493

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 100ul $280 在庫あり
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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse
予測:
Pig(100%), Bovine(100%), Chicken(90%)
クローナリティ:
Polyclonal
特異性:
TM158 Antibody detects endogenous levels of total TM158.
RRID:
AB_2845493
引用形式: Affinity Biosciences Cat# DF12531, RRID:AB_2845493.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

40 kDa BINP binding protein; 40 kDa BINP-binding protein; BBP; BINP receptor; Brain injury derived neurotrophic peptide (BINP) binding protein; Brain specific binding protein; DKFZp586E1621; HBBP; p40BBP; Ras induced senescence protein 1; Ras-induced senescence protein 1; RIS1; TM158_HUMAN; TMEM 158; TMEM158; Transmembrane protein 158;

免疫原

免疫原:

A synthesized peptide derived from human TM158, corresponding to a region within the internal amino acids.

Uniprot:

Q8WZ71(TM158_HUMAN) >>Visit HPA database.

遺伝子(ID):
TMEM158(25907)
タンパク質配列:
MLPLLAALLAAACPLPPVRGGAADAPGLLGVPSNASVNASSADEPIAPRLLASAAPGPPERPGPEEAAAAAAPCNISVQRQMLSSLLVRWGRPRGFQCDLLLFSTNAHGRAFFAAAFHRVGPPLLIEHLGLAAGGAQQDLRLCVGCGWVRGRRTGRLRPAAAPSAAAATAGAPTALPAYPAAEPPGPLWLQGEPLHFCCLDFSLEELQGEPGWRLNRKPIESTLVACFMTLVIVVWSVAALIWPVPIIAGFLPNGMEQRRTTASTTAATPAAVPAGTTAAAAAAAAAAAAAAVTSGVATK

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Bovine
100
Chicken
90
Horse
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Receptor for brain injury-derived neurotrophic peptide (BINP), a synthetic 13-mer peptide.

PTMs:

N-glycosylated.

細胞の位置付け:

Membrane>Multi-pass membrane protein.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the TMEM158 family.

参考文献

1). TMEM158 functions as an oncogene and promotes lung adenocarcinoma progression through the PI3K/AKT pathway via interaction with TWIST1. Experimental cell research, 2024 (PubMed: 38508329) [IF=3.3]

Application: WB    Species: human    Sample:

Fig. 1. Expression patterns of TMEM158 in LUAD patients. (A, B) Volcano plots depicting the DEGs in LUAD were generated utilizing both TCGA-LUAD and GSE140797 datasets. (C) The Venn diagram illustrated the overlapping DEGs between the TCGA-LUAD and GSE140797 datasets. (D, E) In the TCGA-LUAD dataset, TMEM158 expression revealed a significant increase in LUAD tissues in comparison with healthy tissues. (F) The analysis of the GSE140797 dataset has shown that TMEM158 expression is raised in LUAD tissues contrasted to normal tissues. (G) Expression of TMEM158 in different malignancy kinds was analyzed employing data acquired from the TCGA database. (H, I) Analysis of LUAD samples and nearby normal tissues was conducted utilizing western blotting and qRT–PCR. *p < 0.05; **p < 0.01; ***p < 0.001.

Application: IHC    Species: human    Sample: H1650 cells

Fig. 4. TMEM158 promotes migration and invasion while inhibiting cell apoptosis in LUAD cells. (A, B) The apoptotic activities of H1650 cells were assessed using flow cytometry. (C, D) The migratory capability of H1650 cells was assessed employing the wound healing assay. (E, F) The Transwell invasion assay was employed to assess the invasiveness of H1650 cells. *p < 0.05.

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