製品: Phospho-Connexin 43 / GJA1 (Ser262) Antibody
カタログ: AF2307
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Connexin 43 / GJA1 (Ser262)
アプリケーション: WB IHC
反応性: Human
予測: Pig, Bovine, Sheep, Rabbit
分子量: 42, 44, 46kDa; 43kD(Calculated).
ユニプロット: P17302
RRID: AB_2845321

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(83%), Bovine(83%), Sheep(83%), Rabbit(83%)
クローナリティ:
Polyclonal
特異性:
Phospho-Connexin 43 / GJA1 (Ser262) Antibody detects endogenous levels of Connexin 43 / GJA1 only when phosphorylated at Ser262.
RRID:
AB_2845321
引用形式: Affinity Biosciences Cat# AF2307, RRID:AB_2845321.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Connexin 43; Connexin-43; Cx 43; Cx43; CXA1_HUMAN; DFNB38; Gap junction 43 kDa heart protein; Gap junction alpha-1 protein; Gap junction protein alpha 1 43kDa (connexin 43); Gap junction protein alpha 1 43kDa; Gap junction protein alpha like; GJA 1; Gja1; GJAL; ODD; ODDD; ODOD; SDTY3;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
P17302 CXA1_HUMAN:

Expressed in the heart and fetal cochlea.

タンパク質配列:
MGDWSALGKLLDKVQAYSTAGGKVWLSVLFIFRILLLGTAVESAWGDEQSAFRCNTQQPGCENVCYDKSFPISHVRFWVLQIIFVSVPTLLYLAHVFYVMRKEEKLNKKEEELKVAQTDGVNVDMHLKQIEIKKFKYGIEEHGKVKMRGGLLRTYIISILFKSIFEVAFLLIQWYIYGFSLSAVYTCKRDPCPHQVDCFLSRPTEKTIFIIFMLVVSLVSLALNIIELFYVFFKGVKDRVKGKSDPYHATSGALSPAKDCGSQKYAYFNGCSSPTAPLSPMSPPGYKLVTGDRNNSSCRNYNKQASEQNWANYSAEQNRMGQAGSTISNSHAQPFDFPDDNQNSKKLAAGHELQPLAIVDQRPSSRASSRASSRPRPDDLEI

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
83
Bovine
83
Sheep
83
Rabbit
83
Horse
75
Dog
75
Xenopus
75
Chicken
75
Zebrafish
45
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P17302 基板として

Site PTM Type Enzyme
K9 Ubiquitination
K109 Ubiquitination
K128 Ubiquitination
K136 Ubiquitination
Y137 Phosphorylation
K144 Ubiquitination
R148 Methylation
K243 Ubiquitination
S244 Phosphorylation
Y247 Phosphorylation P12931 (SRC)
T250 Phosphorylation
S251 Phosphorylation
S255 Phosphorylation P28482 (MAPK1) , P06493 (CDK1) , P27361 (MAPK3) , Q13164 (MAPK7)
K258 Ubiquitination
S262 Phosphorylation P17252 (PRKCA)
K264 Ubiquitination
Y265 Phosphorylation P12931 (SRC)
Y267 Phosphorylation
S272 Phosphorylation
T275 Phosphorylation
S279 Phosphorylation Q16539 (MAPK14) , P28482 (MAPK1) , P27361 (MAPK3)
S282 Phosphorylation P28482 (MAPK1) , Q16539 (MAPK14) , P27361 (MAPK3) , Q13164 (MAPK7)
Y286 Phosphorylation
K287 Ubiquitination
T290 Phosphorylation
S296 Phosphorylation
S297 Phosphorylation
Y301 Phosphorylation
K303 Ubiquitination
S306 Phosphorylation
Y313 Phosphorylation
S314 Phosphorylation
S325 Phosphorylation P48730 (CSNK1D)
T326 Phosphorylation
S328 Phosphorylation P48730 (CSNK1D)
S330 Phosphorylation P48730 (CSNK1D)
S344 Phosphorylation
K345 Ubiquitination
K346 Ubiquitination
S364 Phosphorylation
S365 Phosphorylation Q02156 (PRKCE) , P17612 (PRKACA)
S368 Phosphorylation P17252 (PRKCA) , P17612 (PRKACA) , Q02156 (PRKCE) , P05771 (PRKCB) , P05129 (PRKCG) , Q05655 (PRKCD)
S369 Phosphorylation P31749 (AKT1) , Q02156 (PRKCE) , P17612 (PRKACA)
S372 Phosphorylation
S373 Phosphorylation P17612 (PRKACA) , P31749 (AKT1) , Q02156 (PRKCE)

研究背景

機能:

Gap junction protein that acts as a regulator of bladder capacity. A gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. May play a critical role in the physiology of hearing by participating in the recycling of potassium to the cochlear endolymph. Negative regulator of bladder functional capacity: acts by enhancing intercellular electrical and chemical transmission, thus sensitizing bladder muscles to cholinergic neural stimuli and causing them to contract (By similarity). May play a role in cell growth inhibition through the regulation of NOV expression and localization. Plays an essential role in gap junction communication in the ventricles (By similarity).

PTMs:

Phosphorylated at Ser-368 by PRKCG; phosphorylation induces disassembly of gap junction plaques and inhibition of gap junction activity (By similarity). Phosphorylation at Ser-325, Ser-328 and Ser-330 by CK1 modulates gap junction assembly. Phosphorylation at Ser-368 by PRKCD triggers its internalization into small vesicles leading to proteasome-mediated degradation (By similarity).

Sumoylated with SUMO1, SUMO2 and SUMO3, which may regulate the level of functional Cx43 gap junctions at the plasma membrane. May be desumoylated by SENP1 or SENP2.

S-nitrosylation at Cys-271 is enriched at the muscle endothelial gap junction in arteries, it augments channel permeability and may regulate of smooth muscle cell to endothelial cell communication.

Acetylated in the developing cortex; leading to delocalization from the cell membrane.

細胞の位置付け:

Cell membrane>Multi-pass membrane protein. Cell junction>Gap junction. Endoplasmic reticulum.
Note: Localizes at the intercalated disk (ICD) in cardiomyocytes and the proper localization at ICD is dependent on TMEM65.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in the heart and fetal cochlea.

サブユニット構造:

A connexon is composed of a hexamer of connexins. Interacts (via C-terminus) with TJP1 (By similarity). Interacts (via C-terminus) with SRC (via SH3 domain) (By similarity). Interacts (not ubiquitinated) with UBQLN4 (via UBA domain) (By similarity). Interacts with SGSM3 and CNST (By similarity). Interacts with RIC1/CIP150. Interacts with CSNK1D. Interacts with NOV. Interacts with TMEM65 (By similarity).

タンパク質ファミリー:

Belongs to the connexin family. Alpha-type (group II) subfamily.

研究領域

· Cellular Processes > Cellular community - eukaryotes > Gap junction.   (View pathway)

· Human Diseases > Cardiovascular diseases > Arrhythmogenic right ventricular cardiomyopathy (ARVC).

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