TRIM14 Antibody - #DF12490

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製品: TRIM14 Antibody
カタログ: DF12490
タンパク質の説明: Rabbit polyclonal antibody to TRIM14
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human
予測: Pig, Bovine, Rabbit
分子量: 50 kDa(Observed); 50kD(Calculated).
ユニプロット: Q14142
RRID: AB_2845295

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#DF12490-BP

TRIM14 Peptide

1mg
関連ダウンロード
Antigen/Blocking Peptide MS Report
Antigen/Blocking Peptide HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(91%), Bovine(90%), Rabbit(100%)
クローナリティ:
Polyclonal
特異性:
TRIM14 Antibody detects endogenous levels of total TRIM14.
RRID:
AB_2845295
引用形式: Affinity Biosciences Cat# DF12490, RRID:AB_2845295.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

5830400N10Rik; KIAA0129; pub; TRI14_HUMAN; TRIM14; tripartite motif protein 14; tripartite motif protein TRIM14; tripartite motif-containing 14; Tripartite motif-containing protein 14;

免疫原

免疫原:

A synthesized peptide derived from human TRIM14, corresponding to a region within C-terminal amino acids.

Uniprot:

Q14142(TRI14_HUMAN) >>Visit HPA database.

遺伝子(ID):
TRIM14(9830)
発現特異性:
Q14142 TRI14_HUMAN:

Highest expression in liver; undetectable in skeletal muscle.

タンパク質配列:
MAGAATGSRTPGRSELVEGCGWRCPEHGDRVAELFCRRCRRCVCALCPVLGAHRGHPVGLALEAAVHVQKLSQECLKQLAIKKQQHIDNITQIEDATEKLKANAESSKTWLKGKFTELRLLLDEEEALAKKFIDKNTQLTLQVYREQADSCREQLDIMNDLSNRVWSISQEPDPVQRLQAYTATEQEMQQQMSLGELCHPVPLSFEPVKSFFKGLVEAVESTLQTPLDIRLKESINCQLSDPSSTKPGTLLKTSPSPERSLLLKYARTPTLDPDTMHARLRLSADRLTVRCGLLGSLGPVPVLRFDALWQVLARDCFATGRHYWEVDVQEAGAGWWVGAAYASLRRRGASAAARLGCNRQSWCLKRYDLEYWAFHDGQRSRLRPRDDLDRLGVFLDYEAGVLAFYDVTGGMSHLHTFRATFQEPLYPALRLWEGAISIPRLP

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Rabbit
100
Pig
91
Bovine
90
Chicken
45
Horse
0
Sheep
0
Dog
0
Xenopus
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Plays a role in the innate immune defense against viruses. Facilitates the type I IFN response by interacting with MAVS at the outer mitochondria membrane and thereby recruiting NF-kappa-B essential modulator IKBKG/NEMO to the MAVS signalosome, leading to the activation of both the IFN regulatory factor 3/IRF3 and NF-kappa-B pathways. Positively regulates the CGAS-induced type I interferon signaling pathway by stabilizing CGAS and inhibiting its autophagic degradation.

PTMs:

Ubiquitinated. Undergoes 'Lys-63'-linked polyubiquitination; this modification allows IKBKG/NEMO recruitment to MAVS.

細胞の位置付け:

Mitochondrion outer membrane.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Highest expression in liver; undetectable in skeletal muscle.

タンパク質ファミリー:

The B-box zinc finger is responsible for inhibition of SPI1-mediated transcriptional activation.

Belongs to the TRIM/RBCC family.

参考文献

1). Circ_RPPH1 facilitates progression of breast cancer via miR-1296-5p/TRIM14 axis. Cancer biology & therapy, 2024 (PubMed: 38816350) [IF=4.4]

Application: WB    Species: human    Sample: MCF-7 and MDA-MB-231 cells

Figure 2. Circ_RPPH1 overexpression promoted BC cell entry into S phase and inhibited cell apoptosis. (a-c) Flow cytometry was used to detect the cycle of BC cells; circ_RPPH1 overexpression [circ_RPPH1(+)] decreased cells in the G0/G1 and G2/M phase, as well as promoting cells entry into the S phase in MCF-7 cells and MDA-MB-231 cells. (d-e) Flow cytometry was also used to detect the apoptosis; circ_RPPH1 (+) inhibited apoptosis in MCF-7 cells and MDA-MB-231 cells. (f) Western blot assay was used to detect TRIM14 protein expression. Circ_RPPH1 (+) increased TRIM14 protein expression in MCF-7 and MDA-MB-231 cells. Data were presented as the mean ± SD, n = 3. ▲▲p 

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