TRAPPC9 Antibody - #DF12489

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製品: TRAPPC9 Antibody
カタログ: DF12489
タンパク質の説明: Rabbit polyclonal antibody to TRAPPC9
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat, Zebrafish
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 128-140 kDa; 129kD(Calculated).
ユニプロット: Q96Q05
RRID: AB_2845294

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Zebrafish
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(83%), Rabbit(100%), Dog(100%), Chicken(83%)
クローナリティ:
Polyclonal
特異性:
TRAPPC9 Antibody detects endogenous levels of total TRAPPC9.
RRID:
AB_2845294
引用形式: Affinity Biosciences Cat# DF12489, RRID:AB_2845294.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

1810044A24Rik; 2900005P22Rik; 4632408O18Rik; Ibp; IKBKBBP; Ikk2 binding protein; KIAA1882; MGC4737; MGC4769; mKIAA1882; MRT13; Nibp; NIK and IKK(beta) binding protein; NIK and IKK{beta} binding protein; RGD1309461; T1; Trafficking protein particle complex 9; TRAPP 120 kDa subunit; TRS130; Tularik gene 1 protein;

免疫原

免疫原:
Uniprot:

Q96Q05(TPPC9_HUMAN) >>Visit HPA database.

遺伝子(ID):
TRAPPC9(83696)
発現特異性:
Q96Q05 TPPC9_HUMAN:

Expressed at high levels in muscle and kidney and to a lower extent in brain, heart and placenta.

タンパク質配列:
MSVPDYMQCAEDHQTLLVVVQPVGIVSEENFFRIYKRICSVSQISVRDSQRVLYIRYRHHYPPENNEWGDFQTHRKVVGLITITDCFSAKDWPQTFEKFHVQKEIYGSTLYDSRLFVFGLQGEIVEQPRTDVAFYPNYEDCQTVEKRIEDFIESLFIVLESKRLDRATDKSGDKIPLLCVPFEKKDFVGLDTDSRHYKKRCQGRMRKHVGDLCLQAGMLQDSLVHYHMSVELLRSVNDFLWLGAALEGLCSASVIYHYPGGTGGKSGARRFQGSTLPAEAANRHRPGAQEVLIDPGALTTNGINPDTSTEIGRAKNCLSPEDIIDKYKEAISYYSKYKNAGVIELEACIKAVRVLAIQKRSMEASEFLQNAVYINLRQLSEEEKIQRYSILSELYELIGFHRKSAFFKRVAAMQCVAPSIAEPGWRACYKLLLETLPGYSLSLDPKDFSRGTHRGWAAVQMRLLHELVYASRRMGNPALSVRHLSFLLQTMLDFLSDQEKKDVAQSLENYTSKCPGTMEPIALPGGLTLPPVPFTKLPIVRHVKLLNLPASLRPHKMKSLLGQNVSTKSPFIYSPIIAHNRGEERNKKIDFQWVQGDVCEVQLMVYNPMPFELRVENMGLLTSGVEFESLPAALSLPAESGLYPVTLVGVPQTTGTITVNGYHTTVFGVFSDCLLDNLPGIKTSGSTVEVIPALPRLQISTSLPRSAHSLQPSSGDEISTNVSVQLYNGESQQLIIKLENIGMEPLEKLEVTSKVLTTKEKLYGDFLSWKLEETLAQFPLQPGKVATFTINIKVKLDFSCQENLLQDLSDDGISVSGFPLSSPFRQVVRPRVEGKPVNPPESNKAGDYSHVKTLEAVLNFKYSGGPGHTEGYYRNLSLGLHVEVEPSVFFTRVSTLPATSTRQCHLLLDVFNSTEHELTVSTRSSEALILHAGECQRMAIQVDKFNFESFPESPGEKGQFANPKQLEEERREARGLEIHSKLGICWRIPSLKRSGEASVEGLLNQLVLEHLQLAPLQWDVLVDGQPCDREAVAACQVGDPVRLEVRLTNRSPRSVGPFALTVVPFQDHQNGVHNYDLHDTVSFVGSSTFYLDAVQPSGQSACLGALLFLYTGDFFLHIRFHEDSTSKELPPSWFCLPSVHVCALEAQA

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Rabbit
100
Sheep
83
Chicken
83
Xenopus
75
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q96Q05 基板として

Site PTM Type Enzyme
S45 Phosphorylation
K98 Ubiquitination
K103 Ubiquitination
K146 Ubiquitination
K174 Ubiquitination
K185 Ubiquitination
S274 Phosphorylation
K315 Ubiquitination
K326 Ubiquitination
Y327 Phosphorylation
K328 Ubiquitination
Y334 Phosphorylation
K336 Ubiquitination
Y337 Phosphorylation
K359 Ubiquitination
K408 Acetylation
K446 Ubiquitination
Y469 Phosphorylation
S480 Phosphorylation
K501 Ubiquitination
K558 Ubiquitination
S559 Phosphorylation
S566 Phosphorylation
T567 Phosphorylation
K568 Ubiquitination
S569 Phosphorylation
Y573 Phosphorylation
S574 Phosphorylation
K748 Ubiquitination
S753 Phosphorylation
K754 Ubiquitination
T757 Phosphorylation
T758 Phosphorylation
K759 Ubiquitination
K761 Ubiquitination
K835 Ubiquitination
K944 Ubiquitination
S953 Phosphorylation
K957 Ubiquitination
K964 Ubiquitination

研究背景

機能:

Functions as an activator of NF-kappa-B through increased phosphorylation of the IKK complex. May function in neuronal cells differentiation. May play a role in vesicular transport from endoplasmic reticulum to Golgi.

細胞の位置付け:

Golgi apparatus>cis-Golgi network. Endoplasmic reticulum. Cytoplasm.
Note: Processes and cell bodies of neurons.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at high levels in muscle and kidney and to a lower extent in brain, heart and placenta.

サブユニット構造:

Component of the multisubunit TRAPP (transport protein particle) complex, which includes at least TRAPPC2, TRAPPC2L, TRAPPC3, TRAPPC3L, TRAPPC4, TRAPPC5, TRAPPC8, TRAPPC9, TRAPPC10, TRAPPC11 and TRAPPC12. Directly interacts with IKBKB and MAP3K14.

タンパク質ファミリー:

Belongs to the NIBP family.

参考文献

1). Synaptic and Genetic Bases of Impaired Motor Learning Associated with Modified Experience-Dependent Cortical Plasticity in Heterozygous Reeler Mutants. Cerebral Cortex, 2022 (PubMed: 34339488) [IF=3.7]

Application: IF/ICC    Species: Mouse    Sample:

Figure 6. Gene enrichment of M1 in WT and HRM. (A) MA plots showing enriched genes in the comparative analyses of (Left) HRM-untrained versus WT-untrained, and (right) HRM-trained versus WT-trained group, identified by the RNA-seq (n = 3 mice). There was no gene enrichment in the comparative analysis of WT-trained versus WT-untrained, and HRM-trained versus HRM-untrained. (B) Left: gene expression levels of Smn1, measured by RNA-sequencing (n = 3 mice, ∗P < 0.05 Wald tests). Right: validation of the gene expression by qRT-PCR, normalized by the geometric mean of the expression levels of Actb and Rps18 (n = 6–8 mice, ∗∗P < 0.01, 1-way ANOVA Tukey’s post hoc tests). (C) Left: gene expression levels of Trappc9, measured by RNA-sequencing. (n = 3 mice, ∗∗P < 0.01 Wald tests). Right: validation of the gene expression by qRT-PCR (n = 8 mice, ∗∗∗P < 0.001, 1-way ANOVA Tukey’s post hoc tests). (D) Smn1 immunohistochemistry on frozen M1 sections, contralateral to the trained forepaw, of trained WT mouse and trained HRM mouse, showing less intensive and less extensive staining in both layers II/III and layer V of trained HRM mouse. Left: cortical layer I to V, scale bar = 100 μm; Middle: layer II/III, scale bar = 50 μm; Right: layer V, scale bar = 50 μm. (E) Trappc9 immunohistochemistry on frozen M1 sections of trained WT mouse and trained HRM mouse, showing less intensive staining in both layers II/III and layer V of trained HRM mouse

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