EXOC5 Antibody - #DF12397

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製品: EXOC5 Antibody
カタログ: DF12397
タンパク質の説明: Rabbit polyclonal antibody to EXOC5
アプリケーション: WB IHC
Cited expt.: WB, IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 77 kDa; 82kD(Calculated).
ユニプロット: O00471
RRID: AB_2845202

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(92%), Bovine(92%), Horse(100%), Sheep(92%), Rabbit(100%), Dog(100%), Chicken(92%)
クローナリティ:
Polyclonal
特異性:
EXOC5 Antibody detects endogenous levels of total EXOC5.
RRID:
AB_2845202
引用形式: Affinity Biosciences Cat# DF12397, RRID:AB_2845202.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AI447711; AI448003; DKFZp666H126; EXOC5; exocyst complex component 5; Exocyst complex component Sec10; Gm76; HSEC10; p71 component of rsec6/8 secretory complex; PRO1912; SEC10; SEC10 like 1; SEC10L1; SEC10P;

免疫原

免疫原:

A synthesized peptide derived from human EXOC5, corresponding to a region within the internal amino acids.

Uniprot:

O00471(EXOC5_HUMAN) >>Visit HPA database.

遺伝子(ID):
EXOC5(10640)
発現特異性:
O00471 EXOC5_HUMAN:

Ubiquitous.

タンパク質配列:
MATTAELFEEPFVADEYIERLVWRTPGGGSRGGPEAFDPKRLLEEFVNHIQELQIMDERIQRKVEKLEQQCQKEAKEFAKKVQELQKSNQVAFQHFQELDEHISYVATKVCHLGDQLEGVNTPRQRAVEAQKLMKYFNEFLDGELKSDVFTNSEKIKEAADIIQKLHLIAQELPFDRFSEVKSKIASKYHDLECQLIQEFTSAQRRGEISRMREVAAVLLHFKGYSHCVDVYIKQCQEGAYLRNDIFEDAGILCQRVNKQVGDIFSNPETVLAKLIQNVFEIKLQSFVKEQLEECRKSDAEQYLKNLYDLYTRTTNLSSKLMEFNLGTDKQTFLSKLIKSIFISYLENYIEVETGYLKSRSAMILQRYYDSKNHQKRSIGTGGIQDLKERIRQRTNLPLGPSIDTHGETFLSQEVVVNLLQETKQAFERCHRLSDPSDLPRNAFRIFTILVEFLCIEHIDYALETGLAGIPSSDSRNANLYFLDVVQQANTIFHLFDKQFNDHLMPLISSSPKLSECLQKKKEIIEQMEMKLDTGIDRTLNCMIGQMKHILAAEQKKTDFKPEDENNVLIQYTNACVKVCAYVRKQVEKIKNSMDGKNVDTVLMELGVRFHRLIYEHLQQYSYSCMGGMLAICDVAEYRKCAKDFKIPMVLHLFDTLHALCNLLVVAPDNLKQVCSGEQLANLDKNILHSFVQLRADYRSARLARHFS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Dog
100
Rabbit
100
Pig
92
Bovine
92
Sheep
92
Chicken
92
Xenopus
73
Zebrafish
58
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Component of the exocyst complex involved in the docking of exocytic vesicles with fusion sites on the plasma membrane.

細胞の位置付け:

Cytoplasm. Midbody.
Note: Localization at the midbody requires the presence of RALA, EXOC2 and EXOC3.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous.

タンパク質ファミリー:

Belongs to the SEC10 family.

参考文献

1). Exocyst controls exosome biogenesis via Rab11a. Molecular Therapy-Nucleic Acids, 2022 (PubMed: 35036064) [IF=6.5]

Application: IHC    Species: Human    Sample: LC tissue

Figure 1 Regulation of exosome release by exocysts in HNC (A and B) Sec10, Sec3, and Exo70 expression in LC tissue compared with that in Ctrl. (A) Representative images of immunohistochemistry staining. (B) Data summary of immunohistochemistry staining. LC, laryngocarcinoma tissue; Ctrl, adjacent normal tissues. ∗∗p < 0.01, ∗∗∗p < 0.001 by t test. Scale bar, 50 μm. (C and D) Sec10, Sec3, and Exo70 expression in HN4 and NP69 cells. (C) Representative images of western blotting. (D) The summary data of western blotting compared with NP69 cells. (E and F) Representative NTA traces and quantification of exosomes after knockdown of the expression of exocyst subunits by transfecting HN4 (E) and CNE2 (F) cells with specific small interfering (si)RNA for Sec10, Sec3, or Exo70 protein. ∗p < 0.05, ∗∗p < 0.05, ∗∗∗p < 0.001 by t test.

Application: WB    Species: Human    Sample: HN4 and NP69 cells

Figure 1 Regulation of exosome release by exocysts in HNC (A and B) Sec10, Sec3, and Exo70 expression in LC tissue compared with that in Ctrl. (A) Representative images of immunohistochemistry staining. (B) Data summary of immunohistochemistry staining. LC, laryngocarcinoma tissue; Ctrl, adjacent normal tissues. ∗∗p < 0.01, ∗∗∗p < 0.001 by t test. Scale bar, 50 μm. (C and D) Sec10, Sec3, and Exo70 expression in HN4 and NP69 cells. (C) Representative images of western blotting. (D) The summary data of western blotting compared with NP69 cells. (E and F) Representative NTA traces and quantification of exosomes after knockdown of the expression of exocyst subunits by transfecting HN4 (E) and CNE2 (F) cells with specific small interfering (si)RNA for Sec10, Sec3, or Exo70 protein. ∗p < 0.05, ∗∗p < 0.05, ∗∗∗p < 0.001 by t test.

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