TTLL6 Antibody - #DF12178

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製品: TTLL6 Antibody
カタログ: DF12178
タンパク質の説明: Rabbit polyclonal antibody to TTLL6
アプリケーション: WB IHC
Cited expt.: WB
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 43-45 kDa(Observed); 96kD(Calculated).
ユニプロット: Q8N841
RRID: AB_2844983

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MS Report
HPLC Report
MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(91%), Zebrafish(91%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
TTL Antibody detects endogenous levels of total TTLL6.
RRID:
AB_2844983
引用形式: Affinity Biosciences Cat# DF12178, RRID:AB_2844983.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

FLJ35808; TTL 6; TTL.6; Tubulin polyglutamylase TTLL6; Tubulin tyrosine ligase like family, member 6;

免疫原

免疫原:

A synthesized peptide derived from human TTLL6, corresponding to a region within N-terminal amino acids.

Uniprot:

Q8N841(TTLL6_HUMAN) >>Visit HPA database.

遺伝子(ID):
TTLL6(284076)
タンパク質配列:
MPQCPTLESQEGENSEEKGDSSKEDPKETVALAFVRENPGAQNGLQNAQQQGKKKRKKKRLVINLSSCRYESVRRAAQQYGFREGGEDDDWTLYWTDYSVSLERVMEMKSYQKINHFPGMSEICRKDLLARNMSRMLKMFPKDFRFFPRTWCLPADWGDLQTYSRSRKNKTYICKPDSGCQGKGIFITRTVKEIKPGEDMICQLYISKPFIIDGFKFDLRIYVLVTSCDPLRIFVYNEGLARFATTSYSRPCTDNLDDICMHLTNYSINKHSSNFSRDAHSGSKRKLSTFSAYLEDHSYNVEQIWRDIEDVIIKTLISAHPIIRHNYHTCFPNHTLNSACFEILGFDILLDHKLKPWLLEVNHSPSFSTDSRLDKEVKDGLLYDTLVLINLESCDKKKVLEEERQRGQFLQQCCSREMRIEEAKGFRAVQLKKTETYEKENCGGFRLIYPSLNSEKYEKFFQDNNSLFQNTVASRAREEYARQLIQELRLKREKKPFQMKKKVEMQGESAGEQVRKKGMRGWQQKQQQKDKAATQASKQYIQPLTLVSYTPDLLLSVRGERKNETDSSLNQEAPTEEASSVFPKLTSAKPFSSLPDLRNINLSSSKLEPSKPNFSIKEAKSASAVNVFTGTVHLTSVETTPESTTQLSISPKSPPTLAVTASSEYSGPETDRVVSFKCKKQQTPPHLTQKKMLKSFLPTKSKSFWESPNTNWTLLKSDMNKPHLISELLTKLQLSGKLSFFPAHYNPKLGMNNLSQNPSLPGECHSRSDSSGEKRQLDVSSLLLQSPQSYNVTLRDLLVIATPAQLDPRPCRSHASAMRDPCMQDQEAYSHCLISGQKGCERS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Pig
91
Zebrafish
91
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Polyglutamylase which preferentially modifies alpha-tubulin. Mediates tubulin polyglutamylation in cilia. Involved in the side-chain elongation step of the polyglutamylation reaction rather than in the initiation step. Generates long side-chains. Generates polyglutamylation of CGAS, leading to impair the DNA-binding activity of CGAS.

細胞の位置付け:

Cell projection>Cilium. Cytoplasm>Cytoskeleton>Cilium basal body.
Note: CEP41 is required for its transport between the basal body and the cilium.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

The flexible c-MTBD (cationic microtubule binding domain) region mediates binding to microtubules. It is positively charged and becomes ordered when bound to microtubules: it interacts with a negatively charged patch on tubulin. The presence of positive charges in the c-MTBD region is essential for proper binding.

Belongs to the tubulin--tyrosine ligase family.

参考文献

1). Repeated Exposure to Sevoflurane in Neonatal Mice Induces Cognitive and Synaptic Impairments in a TTLL6-Mediated Tubulin Polyglutamylation Manner. CNS neuroscience & therapeutics, 2025 (PubMed: 40202105) [IF=4.8]

Application: WB    Species: Mouse    Sample:

FIGURE 3 Effects of repeated sevoflurane exposure on Tau and TTLL6 missorting in hippocampus of neonatal mice. (A) Expression of polyglutamylated Tubulin and TTLL6 in the hippocampus after control or repetitive sevoflurane treatment. (B) Qualification of polyglutamylated tubulin/TTLL6 in different groups; n = 6 mice/group. (C) Expression of AT8 and Tau5 in the hippocampus after control or repetitive sevoflurane treatment. (D) Qualification of AT8/Tau5 in different groups; n = 6 mice/group. (E) Results of microtubule binding assay in the hippocampus of neonatal mice in Control and Sevoflurane groups, (F) the supernatanted protein representing microtubule-unbound Tau, (G) the pelleted protein representing microtubule-bond Tau; n = 3 mice/group. (H) Immunoprecipitation analysis of Tau5 and TTLL6 interaction in hippocampus cells. IgG: Negative control; Input: Total protein and positive control; IB: Immunoblotting; IP: Immunoprecipitation; n = 3 biological replicates. (I) Immunofluorescence of the protein labeled by pTau (red) and TTLL6 (green) in the CA3 area of hippocampus after control or repetitive sevoflurane treatment, repeated sevoflurane treatment increased pTau-TTLL6 co-expressed levels (yellow) in cell body and dendrites (marked with write arrows); blue is DAPI, scale bar: 50 μm. Qualification of (J) pTau and (K) TTLL6 fluorescence intensity in SR area of CA3 region in different groups; n = 3 biological replicates. SR, Stratum radiatum area: Composed mostly of apical dendrites of pyramidal neurons; SP: Stratum pyramidale: Composed mostly of tightly arranged pyramidal cells. *p 

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