Coilin Antibody - #DF12149

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製品: Coilin Antibody
カタログ: DF12149
タンパク質の説明: Rabbit polyclonal antibody to Coilin
アプリケーション: WB IHC
Cited expt.: IHC
反応性: Human, Mouse, Rat
分子量: 80 kDa; 63kD(Calculated).
ユニプロット: P38432
RRID: AB_2844954

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MS Report
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MSDS
説明書
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WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
クローナリティ:
Polyclonal
特異性:
Coilin Antibody detects endogenous levels of total Coilin.
RRID:
AB_2844954
引用形式: Affinity Biosciences Cat# DF12149, RRID:AB_2844954.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CLN 80; CLN80; COIL; COIL_HUMAN; Coilin; Coilin p80; p80; P80 coilin;

免疫原

免疫原:

A synthesized peptide derived from human Coilin, corresponding to a region within the internal amino acids.

Uniprot:

P38432(COIL_HUMAN) >>Visit HPA database.

遺伝子(ID):
COIL(8161)
発現特異性:
P38432 COIL_HUMAN:

Found in all the cell types examined.

タンパク質配列:
MAASETVRLRLQFDYPPPATPHCTAFWLLVDLNRCRVVTDLISLIRQRFGFSSGAFLGLYLEGGLLPPAESARLVRDNDCLRVKLEERGVAENSVVISNGDINLSLRKAKKRAFQLEEGEETEPDCKYSKKHWKSRENNNNNEKVLDLEPKAVTDQTVSKKNKRKNKATCGTVGDDNEEAKRKSPKKKEKCEYKKKAKNPKSPKVQAVKDWANQRCSSPKGSARNSLVKAKRKGSVSVCSKESPSSSSESESCDESISDGPSKVTLEARNSSEKLPTELSKEEPSTKNTTADKLAIKLGFSLTPSKGKTSGTTSSSSDSSAESDDQCLMSSSTPECAAGFLKTVGLFAGRGRPGPGLSSQTAGAAGWRRSGSNGGGQAPGASPSVSLPASLGRGWGREENLFSWKGAKGRGMRGRGRGRGHPVSCVVNRSTDNQRQQQLNDVVKNSSTIIQNPVETPKKDYSLLPLLAAAPQVGEKIAFKLLELTSSYSPDVSDYKEGRILSHNPETQQVDIEILSSLPALREPGKFDLVYHNENGAEVVEYAVTQESKITVFWKELIDPRLIIESPSNTSSTEPA

研究背景

機能:

Component of nuclear coiled bodies, also known as Cajal bodies or CBs, which are involved in the modification and assembly of nucleoplasmic snRNPs.

PTMs:

Symmetrical dimethylation of arginine residues within the RG repeat region enhances affinity for SMN, and thus localization of SMN complexes to CBs.

Phosphorylation during mitosis is associated with disassembly of CBs.

細胞の位置付け:

Nucleus. Nucleus>Cajal body.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Found in all the cell types examined.

タンパク質ファミリー:

The atypical Tudor domain at the C-terminus contains two large unstructured loops, and does not bind methylated residues.

Belongs to the coilin family.

参考文献

1). Predicting and Validating the Regulation of Podocyte Injury and Treatment of Diabetic Kidney Disease by Yinhuo Tang. Journal of visualized experiments : JoVE, 2025 (PubMed: 40622867) [IF=1.2]

Application: IHC    Species: Mouse    Sample:

Figure 2: Amelioration of the histopathological damage of kidneys in DKD mice. (A,B) Renal histology by H&E and PAS staining (scale bar = 20 µm); (C) Masson staining to observe the degree of renal fibrosis and (D) semi-quantitative analysis using ImageJ software; (E-G) Semiquantitative analysis of the relative expression levels of COI-I and α-SMA in renal tissues using IHC and ImageJ software (scale bar = 20 µm). Abbreviations: YHT = yinhuo Tang; DKD = diabetic kidney disease; H&E = Hematoxylin and Eosin stain; PAS = Periodic Acid-Schiff stain; COI-I = Collagen type I; α-SMA = α-smooth muscle actin; CON = control group; MOD = model group; POS = valsartan Positive control group; YH-L = low-dose group of the YHT; YH-M = middle-dose group of the YHT; YH-H = high-dose group of the YHT.

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