製品: GLUD1 Antibody
カタログ: DF12116
タンパク質の説明: Rabbit polyclonal antibody to GLUD1
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Bovine, Sheep, Rabbit, Chicken, Xenopus
分子量: 45-55 kDa; 61kD(Calculated).
ユニプロット: P00367
RRID: AB_2844921

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Bovine(100%), Sheep(100%), Rabbit(100%), Chicken(91%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
GLUD1 Antibody detects endogenous levels of total GLUD1.
RRID:
AB_2844921
引用形式: Affinity Biosciences Cat# DF12116, RRID:AB_2844921.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

AI118167; DHE3_HUMAN; EC 1.4.1.3; GDH 1; GDH; Gdh-X; GDH1; GLUD; Glud1; Glud1a; Glud1b; Gludl; Glutamate dehydrogenase (NAD(P)+); Glutamate dehydrogenase 1; Glutamate dehydrogenase 1 mitochondrial; Glutamate dehydrogenase 1a; Glutamate dehydrogenase 1b; Memory-related gene 2 protein; MGC127177; MGC132003; MGC80801; MGC93608; mitochondrial; MRG-2;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MYRYLGEALLLSRAGPAALGSASADSAALLGWARGQPAAAPQPGLALAARRHYSEAVADREDDPNFFKMVEGFFDRGASIVEDKLVEDLRTRESEEQKRNRVRGILRIIKPCNHVLSLSFPIRRDDGSWEVIEGYRAQHSQHRTPCKGGIRYSTDVSVDEVKALASLMTYKCAVVDVPFGGAKAGVKINPKNYTDNELEKITRRFTMELAKKGFIGPGIDVPAPDMSTGEREMSWIADTYASTIGHYDINAHACVTGKPISQGGIHGRISATGRGVFHGIENFINEASYMSILGMTPGFGDKTFVVQGFGNVGLHSMRYLHRFGAKCIAVGESDGSIWNPDGIDPKELEDFKLQHGSILGFPKAKPYEGSILEADCDILIPAASEKQLTKSNAPRVKAKIIAEGANGPTTPEADKIFLERNIMVIPDLYLNAGGVTVSYFEWLKNLNHVSYGRLTFKYERDSNYHLLMSVQESLERKFGKHGGTIPIVPTAEFQDRISGASEKDIVHSGLAYTMERSARQIMRTAMKYNLGLDLRTAAYVNAIEKVFKVYNEAGVTFT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Bovine
100
Sheep
100
Xenopus
100
Rabbit
100
Chicken
91
Pig
0
Horse
0
Dog
0
Zebrafish
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P00367 基板として

Site PTM Type Enzyme
K68 Acetylation
R76 Methylation
S79 Phosphorylation
K84 Acetylation
K84 Ubiquitination
K98 Acetylation
C112 S-Nitrosylation
S128 Phosphorylation
Y135 Phosphorylation
S153 Phosphorylation
T154 Phosphorylation
S157 Phosphorylation
K162 Acetylation
K162 Ubiquitination
Y170 Phosphorylation
K171 Methylation
K183 Sumoylation
K183 Ubiquitination
K187 Ubiquitination
K191 Ubiquitination
T194 Phosphorylation
K200 Ubiquitination
T206 Phosphorylation
K211 Acetylation
K211 Ubiquitination
K212 Ubiquitination
S227 Phosphorylation
T228 Phosphorylation
K302 Methylation
S316 Phosphorylation
S333 Phosphorylation
S336 Phosphorylation
K346 Acetylation
K352 Acetylation
K352 Ubiquitination
S357 Phosphorylation
K363 Ubiquitination
K365 Ubiquitination
Y367 Phosphorylation
S370 Phosphorylation
S384 Phosphorylation
K386 Ubiquitination
K390 Acetylation
K390 Ubiquitination
K399 Ubiquitination
T409 Phosphorylation
T410 Phosphorylation
K415 Acetylation
K415 Ubiquitination
S438 Phosphorylation
Y439 Phosphorylation
S450 Phosphorylation
Y451 Phosphorylation
K457 Acetylation
S462 Phosphorylation
Y464 Phosphorylation
K480 Acetylation
K480 Ubiquitination
R496 Methylation
K503 Acetylation
K503 Ubiquitination
Y512 Phosphorylation
T513 Phosphorylation
K527 Acetylation
K527 Ubiquitination
Y528 Phosphorylation
Y539 Phosphorylation
K545 Acetylation
K545 Ubiquitination
Y550 Phosphorylation

研究背景

機能:

Mitochondrial glutamate dehydrogenase that converts L-glutamate into alpha-ketoglutarate. Plays a key role in glutamine anaplerosis by producing alpha-ketoglutarate, an important intermediate in the tricarboxylic acid cycle. May be involved in learning and memory reactions by increasing the turnover of the excitatory neurotransmitter glutamate (By similarity).

PTMs:

ADP-ribosylated by SIRT4, leading to inactivate glutamate dehydrogenase activity (By similarity). Stoichiometry shows that ADP-ribosylation occurs in one subunit per catalytically active homohexamer.

細胞の位置付け:

Mitochondrion matrix.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homohexamer.

タンパク質ファミリー:

Belongs to the Glu/Leu/Phe/Val dehydrogenases family.

研究領域

· Cellular Processes > Cell growth and death > Necroptosis.   (View pathway)

· Metabolism > Amino acid metabolism > Arginine biosynthesis.

· Metabolism > Amino acid metabolism > Alanine, aspartate and glutamate metabolism.

· Metabolism > Metabolism of other amino acids > D-Glutamine and D-glutamate metabolism.

· Metabolism > Energy metabolism > Nitrogen metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Metabolism > Global and overview maps > Carbon metabolism.

· Organismal Systems > Excretory system > Proximal tubule bicarbonate reclamation.

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