CCT3 Antibody - #DF12113

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製品: CCT3 Antibody
カタログ: DF12113
タンパク質の説明: Rabbit polyclonal antibody to CCT3
アプリケーション: WB IHC IF/ICC
Cited expt.: WB
反応性: Human, Mouse, Rat, Monkey
予測: Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 60 kDa; 61kD(Calculated).
ユニプロット: P49368
RRID: AB_2844918

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MS Report
HPLC Report
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説明書
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WB Handbook
IHC Protocol
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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat,Monkey
予測:
Zebrafish(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
CCT3 Antibody detects endogenous levels of total CCT3.
RRID:
AB_2844918
引用形式: Affinity Biosciences Cat# DF12113, RRID:AB_2844918.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

CCT 3; CCT gamma; CCT-gamma; CCT3; CCTG; Chaperonin containing TCP1 subunit 3 (gamma); Chaperonin containing TCP1 subunit 3; hTRiC5; PIG48; T complex protein 1 gamma subunit; T complex protein 1 subunit gamma; T-complex protein 1 subunit gamma; TCP 1 gamma; TCP-1-gamma; TCP1 (t complex 1) ring complex polypeptide 5; TCPG_HUMAN; TRIC5;

免疫原

免疫原:

A synthesized peptide derived from human CCT3, corresponding to a region within the internal amino acids.

Uniprot:

P49368(TCPG_HUMAN) >>Visit HPA database.

遺伝子(ID):
CCT3(7203)
タンパク質配列:
MMGHRPVLVLSQNTKRESGRKVQSGNINAAKTIADIIRTCLGPKSMMKMLLDPMGGIVMTNDGNAILREIQVQHPAAKSMIEISRTQDEEVGDGTTSVIILAGEMLSVAEHFLEQQMHPTVVISAYRKALDDMISTLKKISIPVDISDSDMMLNIINSSITTKAISRWSSLACNIALDAVKMVQFEENGRKEIDIKKYARVEKIPGGIIEDSCVLRGVMINKDVTHPRMRRYIKNPRIVLLDSSLEYKKGESQTDIEITREEDFTRILQMEEEYIQQLCEDIIQLKPDVVITEKGISDLAQHYLMRANITAIRRVRKTDNNRIARACGARIVSRPEELREDDVGTGAGLLEIKKIGDEYFTFITDCKDPKACTILLRGASKEILSEVERNLQDAMQVCRNVLLDPQLVPGGGASEMAVAHALTEKSKAMTGVEQWPYRAVAQALEVIPRTLIQNCGASTIRLLTSLRAKHTQENCETWGVNGETGTLVDMKELGIWEPLAVKLQTYKTAVETAVLLLRIDDIVSGHKKKGDDQSRQGGAPDAGQE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Horse
100
Bovine
100
Sheep
100
Dog
100
Zebrafish
100
Rabbit
100
Pig
0
Xenopus
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Component of the chaperonin-containing T-complex (TRiC), a molecular chaperone complex that assists the folding of proteins upon ATP hydrolysis. The TRiC complex mediates the folding of WRAP53/TCAB1, thereby regulating telomere maintenance. As part of the TRiC complex may play a role in the assembly of BBSome, a complex involved in ciliogenesis regulating transports vesicles to the cilia. The TRiC complex plays a role in the folding of actin and tubulin (Probable).

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the TCP-1 chaperonin family.

参考文献

1). Integrated analyzes identify CCT3 as a modulator to shape immunosuppressive tumor microenvironment in lung adenocarcinoma. BMC CANCER, 2023 (PubMed: 36918801) [IF=3.8]

Application: WB    Species: Human    Sample: LUAD A549 cells

Fig. 11 CCT3 knockdown suppressed colony formation and cell proliferation but induced apoptosis of LUAD A549 cells. (a, b) Western blotting (a) and band intensity (b) analyzes of small interfering RNA mediated CCT3 knockdown in A549 cells, GAPDH was used as the loading control. (c, d) Representative colony formation (c) and comparisons of colony counts (d) between si-CCT3 and si-Control cells. (e) The proliferation of si-CCT3 and si-Control A549 cells were determined by WST-8/Cell Counting Kit-8 at 0, 24 and 48 h after cell culturing. (f, g) Representative flow cytometry (f) and comparisons of percentages of total Annexin-V+ cells (g) between si-CCT3 and si-Control A549 cells. Data are representative of three independent experiments. **P 

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