製品: AGGF1 Antibody
カタログ: DF12109
タンパク質の説明: Rabbit polyclonal antibody to AGGF1
アプリケーション: WB IHC
Cited expt.: IHC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Bovine, Horse, Sheep, Dog, Chicken, Xenopus
分子量: 84-100 kDa(Observed); 81kD(Calculated).
ユニプロット: Q8N302
RRID: AB_2844914

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(80%), Bovine(100%), Horse(100%), Sheep(100%), Dog(100%), Chicken(90%), Xenopus(80%)
クローナリティ:
Polyclonal
特異性:
AGGF1 Antibody detects endogenous levels of total AGGF1.
RRID:
AB_2844914
引用形式: Affinity Biosciences Cat# DF12109, RRID:AB_2844914.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

AGGF 1; Aggf1; AGGF1_HUMAN; Angiogenic factor VG5Q; Angiogenic factor with G patch and FHA domains 1; G patch domain containing protein 7; G patch domain-containing protein 7; GPATC 7; GPATC7; GPATCH 7; GPATCH7; HSU84971; HUS84971; hVG5Q; Vasculogenesis gene on 5q; Vasculogenesis gene on 5q protein; VG5Q;

免疫原

免疫原:

A synthesized peptide derived from human AGGF1, corresponding to a region within the internal amino acids.

Uniprot:
遺伝子(ID):
発現特異性:
Q8N302 AGGF1_HUMAN:

Widely expressed. Expressed in endothelial cells, vascular smooth muscle cells and osteoblasts. Expressed in umbilical vein endothelial cells and microvascular endothelial cells.

タンパク質配列:
MASEAPSPPRSPPPPTSPEPELAQLRRKVEKLERELRSCKRQVREIEKLLHHTERLYQNAESNNQELRTQVEELSKILQRGRNEDNKKSDVEVQTENHAPWSISDYFYQTYYNDVSLPNKVTELSDQQDQAIETSILNSKDHLQVENDAYPGTDRTENVKYRQVDHFASNSQEPASALATEDTSLEGSSLAESLRAAAEAAVSQTGFSYDENTGLYFDHSTGFYYDSENQLYYDPSTGIYYYCDVESGRYQFHSRVDLQPYPTSSTKQSKDKKLKKKRKDPDSSATNEEKDLNSEDQKAFSVEHTSCNEEENFANMKKKAKIGIHHKNSPPKVTVPTSGNTIESPLHENISNSTSFKDEKIMETDSEPEEGEITDSQTEDSYDEAITSEGNVTAEDSEDEDEDKIWPPCIRVIVIRSPVLQIGSLFIITAVNPATIGREKDMEHTLRIPEVGVSKFHAEIYFDHDLQSYVLVDQGSQNGTIVNGKQILQPKTKCDPYVLEHGDEVKIGETVLSFHIHPGSDTCDGCEPGQVRAHLRLDKKDESFVGPTLSKEEKELERRKELKKIRVKYGLQNTEYEDEKTLKNPKYKDRAGKRREQVGSEGTFQRDDAPASVHSEITDSNKGRKMLEKMGWKKGEGLGKDGGGMKTPIQLQLRRTHAGLGTGKPSSFEDVHLLQNKNKKNWDKARERFTENFPETKPQKDDPGTMPWVKGTLE

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
90
Xenopus
80
Zebrafish
80
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Promotes angiogenesis and the proliferation of endothelial cells. Able to bind to endothelial cells and promote cell proliferation, suggesting that it may act in an autocrine fashion.

細胞の位置付け:

Cytoplasm. Secreted.
Note: Cytoplasmic in microvascular endothelial cells. Upon angiogenesis, when endothelial cell tube formation is initiated, it is secreted.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Widely expressed. Expressed in endothelial cells, vascular smooth muscle cells and osteoblasts. Expressed in umbilical vein endothelial cells and microvascular endothelial cells.

参考文献

1). The Relationship Between UBE2C and AGGF1 Overexpression and Tumor Angiogenesis in Non-Small Cell Lung Cancer. Cancer Management and Research, 2021 (PubMed: 34354374) [IF=2.5]

Application: IHC    Species: Human    Sample: NSCLC tissues

Figure 1 Immunostaining of UBE2C, AGGF1, VM and MVD in NSCLC and control tissues. (A) Positive staining of UBE2C in NSCLC tissues (×400). (B) Negative staining of UBE2C in control tissues (×400). (C) Positive staining of AGGF1 in NSCLC tissues (×400). (D) Negative staining of AGGF1 in control tissues (×400). (E) Positive staining of VM in NSCLC tissues (×400). (F) Negative staining of VM in control tissues (×400). (G) High MVD in NSCLC tissues (×400). (H) Low MVD in control tissues (×400).

2). Expression and Clinical Significance of the NCAPH, AGGF1, and FOXC2 Proteins in Serous Ovarian Cancer. Cancer Management and Research, 2022 (PubMed: 34584452) [IF=2.5]

Application: IHC    Species: Human    Sample: SOC tissue

Figure 1 The expression of the three proteins in SOC and ovarian serous cystadenoma (ElivisionTM, ×400; (A) NCAPH in SOC; proportion of positive cells; (B) NCAPH in ovarian serous cystadenoma; (C) AGGF1 in SOC; (D) AGGF1 in ovarian serous cystadenoma; (E) FOXC2 in SOC; (F) FOXC2 in ovarian serous cystadenoma).

3). The expression of AGGF1, FOXC2, and E-cadherin in esophageal carcinoma and their clinical significance. Medicine, 2020 (PubMed: 32925786) [IF=1.3]

Application: IHC    Species: human    Sample: esophageal squamous cell carcinoma tissue

Figure 1. |Immunostaining for AGGF1 (angiogenic factor with G-patch and FHA domain 1), FOXC2 (forkhead box C2), and E-cad (E-cadherin) in esophageal squamous cell carcinoma and control tissue. A, Positive AGGF1 in the cytoplasm of esophageal squamous cell carcinoma tissue (40 magnification)

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