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| 製品: | GOLPH3 Antibody |
| カタログ: | DF12099 |
| タンパク質の説明: | Rabbit polyclonal antibody to GOLPH3 |
| アプリケーション: | WB IHC IF/ICC |
| Cited expt.: | WB, IF/ICC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Zebrafish, Bovine, Horse, Sheep, Rabbit, Dog, Chicken, Xenopus |
| 分子量: | 34 kDa(Observed); 34kD(Calculated). |
| ユニプロット: | Q9H4A6 |
| RRID: | AB_2844904 |
Control Products
製品説明
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12099, RRID:AB_2844904.
折りたたみ／展開
Coat protein; Coat protein GPP34; FLJ90675; Golgi associated protein; Golgi peripheral membrane protein 1, 34 kDa; Golgi phosphoprotein 3 (coat protein); Golgi phosphoprotein 3; Golgi protein; GOLP3_HUMAN; Golph3; GOPP1; GPP34; MIDAS; Mitochondrial DNA absence factor;
免疫原
A synthesized peptide derived from human GOLPH3, corresponding to a region within C-terminal amino acids.
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
- Q9H4A6 GOLP3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSLTQRSSGLVQRRTEASRNAADKERAAGGGAGSSEDDAQSRRDEQDDDDKGDSKETRLTLMEEVLLLGLKDREGYTSFWNDCISSGLRGCMLIELALRGRLQLEACGMRRKSLLTRKVICKSDAPTGDVLLDEALKHVKETQPPETVQNWIELLSGETWNPLKLHYQLRNVRERLAKNLVEKGVLTTEKQNFLLFDMTTHPLTNNNIKQRLIKKVQEAVLDKWVNDPHRMDRRLLALIYLAHASDVLENAFAPLLDEQYDLATKRVRQLLDLDPEVECLKANTNEVLWAVVAAFTK
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
研究背景
Phosphatidylinositol-4-phosphate-binding protein that links Golgi membranes to the cytoskeleton and may participate in the tensile force required for vesicle budding from the Golgi. Thereby, may play a role in Golgi membrane trafficking and could indirectly give its flattened shape to the Golgi apparatus. May also bind to the coatomer to regulate Golgi membrane trafficking. May play a role in anterograde transport from the Golgi to the plasma membrane and regulate secretion. Has also been involved in the control of the localization of Golgi enzymes through interaction with their cytoplasmic part. May play an indirect role in cell migration. Has also been involved in the modulation of mTOR signaling. May also be involved in the regulation of mitochondrial lipids biosynthesis.
Phosphorylated.
Golgi apparatus>Golgi stack membrane>Peripheral membrane protein>Cytoplasmic side. Golgi apparatus>trans-Golgi network membrane>Peripheral membrane protein>Cytoplasmic side. Mitochondrion intermembrane space. Cell membrane. Endosome.
Note: Phosphatidylinositol 4-phosphate-binding and oligomerization participate in the recruitment onto Golgi membranes.
Detected in muscle fibers of patients with mitochondrial diseases; not detected in normal muscle fibers.
Belongs to the GOLPH3/VPS74 family.
参考文献
Application: IF/ICC Species: human Sample: Beas-2B cells
Application: WB Species: human Sample: Beas-2B cells
Application: WB Species: human Sample:
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