製品: SQRDL Antibody
カタログ: DF12087
タンパク質の説明: Rabbit polyclonal antibody to SQRDL
アプリケーション: WB IHC IF/ICC
反応性: Human, Mouse, Rat
予測: Pig, Zebrafish, Horse, Sheep, Rabbit, Dog
分子量: 50kd; 50kD(Calculated).
ユニプロット: Q9Y6N5
RRID: AB_2844892

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 100ul $280 在庫あり
 200ul $350 在庫あり

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Zebrafish(86%), Horse(100%), Sheep(88%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
SQRDL Antibody detects endogenous levels of total SQRDL.
RRID:
AB_2844892
引用形式: Affinity Biosciences Cat# DF12087, RRID:AB_2844892.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

0610039J17Rik; 4930557M22Rik; CGI 44; CGI44; mitochondrial; PRO1975; SQRD_HUMAN; Sqrdl; Sulfide dehydrogenase like; Sulfide quinone reductase like; Sulfide quinone reductase-like (yeast); Sulfide:quinone oxidoreductase; Sulfide:quinone oxidoreductase, mitochondrial;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MVPLVAVVSGPRAQLFACLLRLGTQQVGPLQLHTGASHAARNHYEVLVLGGGSGGITMAARMKRKVGAENVAIVEPSERHFYQPIWTLVGAGAKQLSSSGRPTASVIPSGVEWIKARVTELNPDKNCIHTDDDEKISYRYLIIALGIQLDYEKIKGLPEGFAHPKIGSNYSVKTVEKTWKALQDFKEGNAIFTFPNTPVKCAGAPQKIMYLSEAYFRKTGKRSKANIIFNTSLGAIFGVKKYADALQEIIQERNLTVNYKKNLIEVRADKQEAVFENLDKPGETQVISYEMLHVTPPMSPPDVLKTSPVADAAGWVDVDKETLQHRRYPNVFGIGDCTNLPTSKTAAAVAAQSGILDRTISVIMKNQTPTKKYDGYTSCPLVTGYNRVILAEFDYKAEPLETFPFDQSKERLSMYLMKADLMPFLYWNMMLRGYWGGPAFLRKLFHLGMS

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Dog
100
Rabbit
100
Sheep
88
Zebrafish
86
Xenopus
75
Bovine
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q9Y6N5 基板として

Site PTM Type Enzyme
K115 Acetylation
K125 Ubiquitination
K135 Acetylation
S137 Phosphorylation
Y151 Phosphorylation
K155 Ubiquitination
K165 Ubiquitination
K173 Acetylation
K173 Ubiquitination
K177 Ubiquitination
K180 Acetylation
K186 Acetylation
K186 Ubiquitination
T197 Phosphorylation
Y259 Phosphorylation
K260 Acetylation
K261 Ubiquitination
K320 Acetylation
T338 Phosphorylation
T342 Phosphorylation
S343 Phosphorylation
S361 Phosphorylation
Y395 Phosphorylation
S408 Phosphorylation
K409 Ubiquitination
S413 Phosphorylation
Y415 Phosphorylation
K443 Acetylation
S450 Phosphorylation

研究背景

機能:

Catalyzes the oxidation of hydrogen sulfide with the help of a quinone, such as ubiquinone, giving rise to thiosulfate and ultimately to sulfane (molecular sulfur) atoms. Requires an additional electron acceptor; can use sulfite, sulfide or cyanide (in vitro).

細胞の位置付け:

Mitochondrion.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the SQRD family.

研究領域

· Metabolism > Energy metabolism > Sulfur metabolism.

参考文献

1). Clinical significance of Interleukin 17 receptor E in diabetic nephropathy. International Immunopharmacology (PubMed: 37235960) [IF=5.6]

Application: IF/ICC    Species: Human    Sample: renal biopsy tissues

Fig. 4. Immunofluorescence double staining of IL-17RE and specific tubular/glomerular markers in renal biopsy tissues of patients with DN. IL-17RE was marked with FITC-conjugated secondary antibody; proximal tubular epithelial, distal tubular epithelial, and collecting duct cells were marked as AQP1, CD28k, and AQP3 with Cy3-conjugated secondary antibody, respectively; glomerular podocytes and endothelial and mesangial cells were marked as synaptopodin, CD31, and PDGFR-β with Cy3-conjugated secondary antibody, respectively. The white arrow indicates the positive staining area. NGAL, AQP1, CD28k, and AQP3: scale bar = 50 μm; synaptopodin, CD31, and PDGFR-β: scale bar = 25 μm. AQP, Aquaporin; CD28k, Calbindin D28k; PDGFR-β, platelet-derived growth factor receptors beta.

Application: IHC    Species: Human    Sample: renal biopsy tissues

Fig. 5. Pathological staining and protein expression levels in mouse kidney tissues. (A) H&E staining of the mouse kidneys. Scale bar = 50 μm. (B) PAS staining of mouse kidneys. Scale bar = 50 μm. (C) IHC assay of NGAL protein expression in the mouse kidneys. Scale bar = 50 μm. (D) IHC assay of IL-17RE protein expression in the mouse kidneys. Scale bar = 50 μm. The results represent the mean ± SD for six samples/group. *P < 0.05, ***P < 0.001 vs db/m. H&E, haematoxylin and eosin staining; PAS, periodic acid-Schiff staining; IHC, immunohistochemical staining.

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