RNase H1 Antibody - #DF12084

Figure 1. Germ cell-specific Rnaseh1 knockout impairs mouse spermatogenesis and causes male infertility. (A) Schematic diagram for the construction of the Rnaseh1F/F and Stra8-Rnaseh1−/− mice. The mouse Rnaseh1 locus surrounding exons 2, 3 and 4 is shown in the wild-type allele panel. (B) The genotyping of the Rnaseh1+/+, Rnaseh1F/+, and Rnaseh1F/F mice. (C) The RNase H1 protein levels are decreased in Stra8-Rnaseh1−/−testes. Immunoblotting of RNase H1 is performed in Rnaseh1F/F and Stra8-Rnaseh1−/− testes. H3 and Tubulin serves as the loading control. (D) The quantification of RNase H1 protein levels in (C) (n = 5 independent experiments). Black dots indicate Rnaseh1F/F mice, and red dots indicate Stra8-Rnaseh1−/− mice. Data are presented as means ± SEM. two-tailed Student's t-test; **P < 0.01. (E and F) Germ cell-specific knockout of Rnaseh1 causes male infertility. The fertility assessment experiments are performed in Rnaseh1F/F and Stra8-Rnaseh1−/− male mice. Pregnancy rates (%) of plugged wild-type female mice after mating with Rnaseh1F/F and Stra8-Rnaseh1−/− male mice (n = 3 independent experiments) (E). Average litter sizes were observed with Rnaseh1F/F and Stra8-Rnaseh1−/− male mice (n = 3 independent experiments) (F). Black dots indicate Rnaseh1F/F mice, and red dots indicate Stra8-Rnaseh1−/− mice. Data are presented as means ± SEM. two-tailed Student's t-test; ***P < 0.001. (G) The Stra8-Rnaseh1−/− mouse testis is smaller than that of the Rnaseh1F/F mouse. (H) Ratio of testis weight/body weight in Rnaseh1F/F and Stra8-Rnaseh1−/− mice (n = 4 independent experiments). Black dots indicate Rnaseh1F/F mice and red dots indicate Stra8-Rnaseh1−/− mice. Data are presented as means ± SEM. two-tailed Student's t-test; *P < 0.05. (I) Histological analysis of the seminiferous tubules and caudal epididymis of the Rnaseh1F/F and Stra8-Rnaseh1−/− mice by hematoxylin and eosin staining. Arrowheads indicate dead cells. (J) Sperm counts from the cauda epididymis significantly decreased in the Stra8-Rnaseh1−/− mice compared with Rnaseh1F/F mice. (n = 5 independent experiments). Black dots indicate Rnaseh1F/F mice, and red dots indicate Stra8-Rnaseh1−/− mice.