PLOD3 Antibody - #DF12034
| 製品: | PLOD3 Antibody |
| カタログ: | DF12034 |
| タンパク質の説明: | Rabbit polyclonal antibody to PLOD3 |
| アプリケーション: | WB IHC |
| 反応性: | Human, Mouse, Rat |
| 予測: | Pig, Horse, Sheep, Rabbit, Dog |
| 分子量: | 80-85 kDa; 85kD(Calculated). |
| ユニプロット: | O60568 |
| RRID: | AB_2844839 |
製品説明
*The optimal dilutions should be determined by the end user.
*Tips:
WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.
引用形式: Affinity Biosciences Cat# DF12034, RRID:AB_2844839.
折りたたみ／展開
2-oxoglutarate 5-dioxygenase 3; bone protein I; BP-I; LH3; Lysine hydroxylase 3; Lysyl hydroxylase 3; Plod3; PLOD3_HUMAN; Procollagen lysine,2 oxoglutarate 5 dioxygenase 3 precursor; Procollagen-lysine;
免疫原
Ubiquitous (PubMed:9724729). Detected in heart, placenta and pancreas and at lower levels in lung, liver and skeletal muscle (PubMed:9582318, PubMed:9724729).
- O60568 PLOD3_HUMAN:
- Protein BLAST With
- NCBI/
- ExPASy/
- Uniprot
MTSSGPGPRFLLLLPLLLPPAASASDRPRGRDPVNPEKLLVITVATAETEGYLRFLRSAEFFNYTVRTLGLGEEWRGGDVARTVGGGQKVRWLKKEMEKYADREDMIIMFVDSYDVILAGSPTELLKKFVQSGSRLLFSAESFCWPEWGLAEQYPEVGTGKRFLNSGGFIGFATTIHQIVRQWKYKDDDDDQLFYTRLYLDPGLREKLSLNLDHKSRIFQNLNGALDEVVLKFDRNRVRIRNVAYDTLPIVVHGNGPTKLQLNYLGNYVPNGWTPEGGCGFCNQDRRTLPGGQPPPRVFLAVFVEQPTPFLPRFLQRLLLLDYPPDRVTLFLHNNEVFHEPHIADSWPQLQDHFSAVKLVGPEEALSPGEARDMAMDLCRQDPECEFYFSLDADAVLTNLQTLRILIEENRKVIAPMLSRHGKLWSNFWGALSPDEYYARSEDYVELVQRKRVGVWNVPYISQAYVIRGDTLRMELPQRDVFSGSDTDPDMAFCKSFRDKGIFLHLSNQHEFGRLLATSRYDTEHLHPDLWQIFDNPVDWKEQYIHENYSRALEGEGIVEQPCPDVYWFPLLSEQMCDELVAEMEHYGQWSGGRHEDSRLAGGYENVPTVDIHMKQVGYEDQWLQLLRTYVGPMTESLFPGYHTKARAVMNFVVRYRPDEQPSLRPHHDSSTFTLNVALNHKGLDYEGGGCRFLRYDCVISSPRKGWALLHPGRLTHYHEGLPTTWGTRYIMVSFVDP
種類予測
Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.
High(score>80) Medium(80>score>50) Low(score<50) No confidence
PTMs - O60568 基板として
| Site | PTM Type | Enzyme | Source |
|---|---|---|---|
| S23 | Phosphorylation | Uniprot | |
| S25 | Phosphorylation | Uniprot | |
| Y52 | Phosphorylation | Uniprot | |
| N63 | N-Glycosylation | Uniprot | |
| R76 | Methylation | Uniprot | |
| K127 | Acetylation | Uniprot | |
| K128 | Acetylation | Uniprot | |
| K128 | Ubiquitination | Uniprot | |
| S134 | Phosphorylation | Uniprot | |
| T174 | Phosphorylation | Uniprot | |
| T175 | Phosphorylation | Uniprot | |
| K186 | Ubiquitination | Uniprot | |
| K207 | Ubiquitination | Uniprot | |
| K215 | Ubiquitination | Uniprot | |
| K232 | Ubiquitination | Uniprot | |
| T288 | Phosphorylation | Uniprot | |
| S367 | Phosphorylation | Uniprot | |
| R411 | Methylation | Uniprot | |
| S419 | Phosphorylation | Uniprot | |
| Y444 | Phosphorylation | Uniprot | |
| Y460 | Phosphorylation | Uniprot | |
| K495 | Ubiquitination | Uniprot | |
| K500 | Ubiquitination | Uniprot | |
| N548 | N-Glycosylation | Uniprot | |
| K645 | Ubiquitination | Uniprot | |
| Y730 | Phosphorylation | Uniprot |
研究背景
Multifunctional enzyme that catalyzes a series of essential post-translational modifications on Lys residues in procollagen. Plays a redundant role in catalyzing the formation of hydroxylysine residues in -Xaa-Lys-Gly- sequences in collagens. Plays a redundant role in catalyzing the transfer of galactose onto hydroxylysine groups, giving rise to galactosyl 5-hydroxylysine. Has an essential role by catalyzing the subsequent transfer of glucose moieties, giving rise to 1,2-glucosylgalactosyl-5-hydroxylysine residues. Catalyzes hydroxylation and glycosylation of Lys residues in the MBL1 collagen-like domain, giving rise to hydroxylysine and 1,2-glucosylgalactosyl-5-hydroxylysine residues. Essential for normal biosynthesis and secretion of type IV collagens (Probable). Essential for normal formation of basement membranes (By similarity).
Rough endoplasmic reticulum. Endoplasmic reticulum lumen. Endoplasmic reticulum membrane>Peripheral membrane protein>Lumenal side. Secreted. Secreted>Extracellular space.
Note: The majority of the secreted protein is associated with the extracellular matrix.
Ubiquitous. Detected in heart, placenta and pancreas and at lower levels in lung, liver and skeletal muscle.
Homodimer.
The N-terminal domain mediates glycosyltransferase activity.
The C-terminal domain that mediates lysyl hydroxylase activity is also important for homodimerization.
研究領域
· Metabolism > Amino acid metabolism > Lysine degradation.
· Metabolism > Glycan biosynthesis and metabolism > Other types of O-glycan biosynthesis.
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