製品: PLD1 Antibody
カタログ: AF4765
タンパク質の説明: Rabbit polyclonal antibody to PLD1
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Dog
分子量: 120kDa; 124kD(Calculated).
ユニプロット: Q13393
RRID: AB_2844759

類似製品を見る>>

   サイズ 価格 在庫状況
 50ul $250 在庫あり
 100ul $350 在庫あり
 200ul $450 在庫あり

リードタイム: 当日配達

For pricing and ordering contact:
お問い合わせ先

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(92%), Bovine(92%), Horse(92%), Sheep(92%), Dog(92%)
クローナリティ:
Polyclonal
特異性:
PLD1 Antibody detects endogenous levels of total PLD1.
RRID:
AB_2844759
引用形式: Affinity Biosciences Cat# AF4765, RRID:AB_2844759.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

Choline phosphatase 1; hPLD1; Phosphatidylcholine-hydrolyzing phospholipase D1; Phospholipase D1; PLD 1; PLD1; PLD1_HUMAN;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q13393 PLD1_HUMAN:

Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.

タンパク質配列:
MSLKNEPRVNTSALQKIAADMSNIIENLDTRELHFEGEEVDYDVSPSDPKIQEVYIPFSAIYNTQGFKEPNIQTYLSGCPIKAQVLEVERFTSTTRVPSINLYTIELTHGEFKWQVKRKFKHFQEFHRELLKYKAFIRIPIPTRRHTFRRQNVREEPREMPSLPRSSENMIREEQFLGRRKQLEDYLTKILKMPMYRNYHATTEFLDISQLSFIHDLGPKGIEGMIMKRSGGHRIPGLNCCGQGRACYRWSKRWLIVKDSFLLYMKPDSGAIAFVLLVDKEFKIKVGKKETETKYGIRIDNLSRTLILKCNSYRHARWWGGAIEEFIQKHGTNFLKDHRFGSYAAIQENALAKWYVNAKGYFEDVANAMEEANEEIFITDWWLSPEIFLKRPVVEGNRWRLDCILKRKAQQGVRIFIMLYKEVELALGINSEYTKRTLMRLHPNIKVMRHPDHVSSTVYLWAHHEKLVIIDQSVAFVGGIDLAYGRWDDNEHRLTDVGSVKRVTSGPSLGSLPPAAMESMESLRLKDKNEPVQNLPIQKSIDDVDSKLKGIGKPRKFSKFSLYKQLHRHHLHDADSISSIDSTSSYFNHYRSHHNLIHGLKPHFKLFHPSSESEQGLTRPHADTGSIRSLQTGVGELHGETRFWHGKDYCNFVFKDWVQLDKPFADFIDRYSTPRMPWHDIASAVHGKAARDVARHFIQRWNFTKIMKSKYRSLSYPFLLPKSQTTAHELRYQVPGSVHANVQLLRSAADWSAGIKYHEESIHAAYVHVIENSRHYIYIENQFFISCADDKVVFNKIGDAIAQRILKAHRENQKYRVYVVIPLLPGFEGDISTGGGNALQAIMHFNYRTMCRGENSILGQLKAELGNQWINYISFCGLRTHAELEGNLVTELIYVHSKLLIADDNTVIIGSANINDRSMLGKRDSEMAVIVQDTETVPSVMDGKEYQAGRFARGLRLQCFRVVLGYLDDPSEDIQDPVSDKFFKEVWVSTAARNATIYDKVFRCLPNDEVHNLIQLRDFINKPVLAKEDPIRAEEELKKIRGFLVQFPFYFLSEESLLPSVGTKEAIVPMEVWT

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
92
Horse
92
Bovine
92
Sheep
92
Dog
92
Xenopus
58
Zebrafish
0
Chicken
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13393 基板として

Site PTM Type Enzyme
S2 Phosphorylation P17252 (PRKCA)
S12 Phosphorylation
K16 Ubiquitination
S22 Phosphorylation
Y42 Phosphorylation
S45 Phosphorylation
K50 Ubiquitination
K121 Ubiquitination
K132 Methylation
T147 Phosphorylation P17252 (PRKCA)
S162 Phosphorylation
K189 Ubiquitination
K294 Ubiquitination
K406 Methylation
S431 Phosphorylation
T495 Phosphorylation
S499 Phosphorylation
K501 Ubiquitination
S505 Phosphorylation P54646 (PRKAA2)
S508 Phosphorylation
S519 Phosphorylation
S522 Phosphorylation
S540 Phosphorylation
K547 Ubiquitination
S558 Phosphorylation
K559 Ubiquitination
S561 Phosphorylation P17252 (PRKCA)
Y586 Phosphorylation
Y590 Phosphorylation
S610 Phosphorylation P68400 (CSNK2A1)
S611 Phosphorylation P68400 (CSNK2A1)
S629 Phosphorylation
K662 Ubiquitination
K688 Ubiquitination
Y711 Phosphorylation
K796 Ubiquitination
K807 Acetylation
K1000 Ubiquitination
K1027 Ubiquitination

研究背景

機能:

Implicated as a critical step in numerous cellular pathways, including signal transduction, membrane trafficking, and the regulation of mitosis. May be involved in the regulation of perinuclear intravesicular membrane traffic (By similarity).

細胞の位置付け:

Cytoplasm>Perinuclear region. Endoplasmic reticulum membrane>Lipid-anchor>Cytoplasmic side. Golgi apparatus membrane>Lipid-anchor>Cytoplasmic side. Late endosome membrane>Lipid-anchor>Cytoplasmic side.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed abundantly in the pancreas and heart and at high levels in brain, placenta, spleen, uterus and small intestine.

サブユニット構造:

Interacts with PIP5K1B.

タンパク質ファミリー:

Belongs to the phospholipase D family.

研究領域

· Cellular Processes > Transport and catabolism > Endocytosis.   (View pathway)

· Environmental Information Processing > Signal transduction > Ras signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > cAMP signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Sphingolipid signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Phospholipase D signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Pancreatic cancer.   (View pathway)

· Human Diseases > Cancers: Overview > Choline metabolism in cancer.   (View pathway)

· Metabolism > Lipid metabolism > Glycerophospholipid metabolism.

· Metabolism > Lipid metabolism > Ether lipid metabolism.

· Metabolism > Global and overview maps > Metabolic pathways.

· Organismal Systems > Immune system > Fc gamma R-mediated phagocytosis.   (View pathway)

· Organismal Systems > Nervous system > Glutamatergic synapse.

Restrictive clause

 

Affinity Biosciences tests all products strictly. Citations are provided as a resource for additional applications that have not been validated by Affinity Biosciences. Please choose the appropriate format for each application and consult Materials and Methods sections for additional details about the use of any product in these publications.

For Research Use Only.
Not for use in diagnostic or therapeutic procedures. Not for resale. Not for distribution without written consent. Affinity Biosciences will not be held responsible for patent infringement or other violations that may occur with the use of our products. Affinity Biosciences, Affinity Biosciences Logo and all other trademarks are the property of Affinity Biosciences LTD.