製品: STK3 Antibody
カタログ: AF4614
タンパク質の説明: Rabbit polyclonal antibody to STK3
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog
分子量: 56kDa,52kDa; 56kD(Calculated).
ユニプロット: Q13188
RRID: AB_2844652

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%)
クローナリティ:
Polyclonal
特異性:
STK3 Antibody detects endogenous levels of total STK3.
RRID:
AB_2844652
引用形式: Affinity Biosciences Cat# AF4614, RRID:AB_2844652.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

0610042I06Rik; EC 2.7.11.1; FLJ90748; KB 1458E12.1; Kinase responsive to stress 1; KRS1; Mammalian STE20 like protein kinase 2; Mammalian STE20-like protein kinase 2; Mammalian sterile 20-like 2; Mess1; MST; MST-2; MST2; Mst3; Serine/threonine kinase 3 (STE20 homolog, yeast); Serine/threonine kinase 3 (Ste20, yeast homolog); Serine/threonine kinase 3; Serine/threonine protein kinase 3; Serine/threonine protein kinase Krs1; Serine/threonine-protein kinase 3; Serine/threonine-protein kinase Krs-1; STE20 like kinase MST2; STE20-like kinase MST2; Stk3; STK3_HUMAN; wu:fc19e11; zgc:55383;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q13188 STK3_HUMAN:

Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.

タンパク質配列:
MEQPPAPKSKLKKLSEDSLTKQPEEVFDVLEKLGEGSYGSVFKAIHKESGQVVAIKQVPVESDLQEIIKEISIMQQCDSPYVVKYYGSYFKNTDLWIVMEYCGAGSVSDIIRLRNKTLIEDEIATILKSTLKGLEYLHFMRKIHRDIKAGNILLNTEGHAKLADFGVAGQLTDTMAKRNTVIGTPFWMAPEVIQEIGYNCVADIWSLGITSIEMAEGKPPYADIHPMRAIFMIPTNPPPTFRKPELWSDDFTDFVKKCLVKNPEQRATATQLLQHPFIKNAKPVSILRDLITEAMEIKAKRHEEQQRELEEEEENSDEDELDSHTMVKTSVESVGTMRATSTMSEGAQTMIEHNSTMLESDLGTMVINSEDEEEEDGTMKRNATSPQVQRPSFMDYFDKQDFKNKSHENCNQNMHEPFPMSKNVFPDNWKVPQDGDFDFLKNLSLEELQMRLKALDPMMEREIEELRQRYTAKRQPILDAMDAKKRRQQNF

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
100
Xenopus
0
Zebrafish
0
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13188 基板として

Site PTM Type Enzyme
M1 Acetylation
K13 Ubiquitination
S15 Phosphorylation P53350 (PLK1)
S18 Phosphorylation P53350 (PLK1)
K21 Ubiquitination
K32 Ubiquitination
K43 Ubiquitination
K47 Ubiquitination
K56 Ubiquitination
Y81 Phosphorylation P00519 (ABL1)
K116 Ubiquitination
T117 Phosphorylation Q9Y243 (AKT3) , P31751 (AKT2) , P31749 (AKT1)
K128 Ubiquitination
K148 Ubiquitination
K161 Ubiquitination
T174 Phosphorylation
K177 Ubiquitination
T180 Phosphorylation Q13188 (STK3) , Q7L7X3 (TAOK1)
T235 Phosphorylation
T240 Phosphorylation
K243 Ubiquitination
K282 Ubiquitination
K298 Ubiquitination
S316 Phosphorylation P53350 (PLK1)
S323 Phosphorylation
T325 Phosphorylation
S333 Phosphorylation
T336 Phosphorylation
S369 Phosphorylation
T378 Phosphorylation Q13188 (STK3)
T384 Phosphorylation P31749 (AKT1)
S385 Phosphorylation P06493 (CDK1)
S392 Phosphorylation
Y396 Phosphorylation
S406 Phosphorylation
C410 S-Nitrosylation
K430 Ubiquitination
S444 Phosphorylation
K453 Ubiquitination

PTMs - Q13188 酵素として

Substrate Site Source
O95835 (LATS1) S872 Uniprot
O95835 (LATS1) S909 Uniprot
O95835 (LATS1) T967 Uniprot
O95835 (LATS1) T1012 Uniprot
O95835 (LATS1) T1060 Uniprot
O95835 (LATS1) T1079 Uniprot
P00519 (ABL1) T735 Uniprot
P17252 (PRKCA) S226 Uniprot
P17252 (PRKCA) T228 Uniprot
P18754 (RCC1) S2 Uniprot
P18754 (RCC1) S11 Uniprot
P46937 (YAP1) S127 Uniprot
P51955 (NEK2) S356 Uniprot
P51955 (NEK2) S365 Uniprot
P51955 (NEK2) S406 Uniprot
P51955 (NEK2) S438 Uniprot
Q13188 (STK3) T180 Uniprot
Q13188 (STK3) T378 Uniprot
Q7L9L4 (MOB1B) T12 Uniprot
Q7L9L4 (MOB1B) T35 Uniprot
Q7L9L4 (MOB1B) T74 Uniprot
Q9H492 (MAP1LC3A) T50 Uniprot
Q9H4B6 (SAV1) T26 Uniprot
Q9H4B6 (SAV1) S27 Uniprot
Q9H4B6 (SAV1) S36 Uniprot
Q9H4B6 (SAV1) S269 Uniprot
Q9H8S9 (MOB1A) T12 Uniprot
Q9H8S9 (MOB1A) T35 Uniprot
Q9H8S9 (MOB1A) T74 Uniprot
Q9NRM7 (LATS2) S872 Uniprot
Q9NRM7 (LATS2) T1041 Uniprot

研究背景

機能:

Stress-activated, pro-apoptotic kinase which, following caspase-cleavage, enters the nucleus and induces chromatin condensation followed by internucleosomal DNA fragmentation. Key component of the Hippo signaling pathway which plays a pivotal role in organ size control and tumor suppression by restricting proliferation and promoting apoptosis. The core of this pathway is composed of a kinase cascade wherein STK3/MST2 and STK4/MST1, in complex with its regulatory protein SAV1, phosphorylates and activates LATS1/2 in complex with its regulatory protein MOB1, which in turn phosphorylates and inactivates YAP1 oncoprotein and WWTR1/TAZ. Phosphorylation of YAP1 by LATS2 inhibits its translocation into the nucleus to regulate cellular genes important for cell proliferation, cell death, and cell migration. STK3/MST2 and STK4/MST1 are required to repress proliferation of mature hepatocytes, to prevent activation of facultative adult liver stem cells (oval cells), and to inhibit tumor formation. Phosphorylates NKX2-1 (By similarity). Phosphorylates NEK2 and plays a role in centrosome disjunction by regulating the localization of NEK2 to centrosome, and its ability to phosphorylate CROCC and CEP250. In conjunction with SAV1, activates the transcriptional activity of ESR1 through the modulation of its phosphorylation. Positively regulates RAF1 activation via suppression of the inhibitory phosphorylation of RAF1 on 'Ser-259'. Phosphorylates MOBKL1A and RASSF2. Phosphorylates MOBKL1B on 'Thr-74'. Acts cooperatively with MOBKL1B to activate STK38.

PTMs:

Phosphorylation at Thr-117 and Thr-384 by PKB/AKT1, leads to inhibition of its: cleavage, kinase activity, autophosphorylation at Thr-180, binding to RASSF1 and nuclear translocation, and increase in its binding to RAF1.

Proteolytically cleaved by caspase-3 during apoptosis. Proteolytic cleavage results in kinase activation and nuclear translocation of the truncated form (MST1/N).

細胞の位置付け:

Cytoplasm. Nucleus.
Note: The caspase-cleaved form cycles between nucleus and cytoplasm (By similarity). Phosphorylation at Thr-117 leads to inhibition of nuclear translocation.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed at high levels in adult kidney, skeletal and placenta tissues and at very low levels in adult heart, lung and brain tissues.

サブユニット構造:

Homodimer; mediated via the coiled-coil region. Interacts with NORE1, which inhibits autoactivation (By similarity). Interacts with and stabilizes SAV1. Interacts with RAF1, which prevents dimerization and phosphorylation. Interacts with RASSF1. Interacts (via SARAH domain) with isoform 1 of NEK2. Interacts with ESR1 only in the presence of SAV1. Interacts with PKB/AKT1. Forms a tripartite complex with MOBKL1B and STK38. Interacts with RASSF2 (via SARAH domain). Interacts with DLG5 (via PDZ domain 3). Interacts with LATS1; this interaction is inhibited in the presence of DLG5. Interacts with MARK3 in the presence of DLG5.

タンパク質ファミリー:

Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily.

研究領域

· Environmental Information Processing > Signal transduction > MAPK signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Hippo signaling pathway - multiple species.   (View pathway)

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