製品: CtBP1/2 Antibody
カタログ: DF10353
タンパク質の説明: Rabbit polyclonal antibody to CtBP1/2
アプリケーション: WB
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Dog, Chicken, Xenopus
分子量: 48KD; 48kD,49kD(Calculated).
ユニプロット: Q13363 | P56545
RRID: AB_2844360

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Dog(100%), Chicken(100%), Xenopus(100%)
クローナリティ:
Polyclonal
特異性:
CtBP1/2 Antibody detects endogenous levels of total CtBP1/2.
RRID:
AB_2844360
引用形式: Affinity Biosciences Cat# DF10353, RRID:AB_2844360.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

BARS; brefeldin A- ribosylated substrate; C terminal binding protein 1; C-terminal-binding protein 1; CTBP; CtBP1; CTBP1_HUMAN; MGC104684; C terminal binding protein 2; C-terminal-binding protein 2; CtBP2; CTBP2_HUMAN; ribeye;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
発現特異性:
Q13363 CTBP1_HUMAN:

Expressed in germinal center B-cells.

P56545 CTBP2_HUMAN:

Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.

タンパク質配列:
MGSSHLLNKGLPLGVRPPIMNGPLHPRPLVALLDGRDCTVEMPILKDVATVAFCDAQSTQEIHEKVLNEAVGALMYHTITLTREDLEKFKALRIIVRIGSGFDNIDIKSAGDLGIAVCNVPAASVEETADSTLCHILNLYRRATWLHQALREGTRVQSVEQIREVASGAARIRGETLGIIGLGRVGQAVALRAKAFGFNVLFYDPYLSDGVERALGLQRVSTLQDLLFHSDCVTLHCGLNEHNHHLINDFTVKQMRQGAFLVNTARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFSQGPLKDAPNLICTPHAAWYSEQASIEMREEAAREIRRAITGRIPDSLKNCVNKDHLTAATHWASMDPAVVHPELNGAAYRYPPGVVGVAPTGIPAAVEGIVPSAMSLSHGLPPVAHPPHAPSPGQTVKPEADRDHASDQL

MALVDKHKVKRQRLDRICEGIRPQIMNGPLHPRPLVALLDGRDCTVEMPILKDLATVAFCDAQSTQEIHEKVLNEAVGAMMYHTITLTREDLEKFKALRVIVRIGSGYDNVDIKAAGELGIAVCNIPSAAVEETADSTICHILNLYRRNTWLYQALREGTRVQSVEQIREVASGAARIRGETLGLIGFGRTGQAVAVRAKAFGFSVIFYDPYLQDGIERSLGVQRVYTLQDLLYQSDCVSLHCNLNEHNHHLINDFTIKQMRQGAFLVNAARGGLVDEKALAQALKEGRIRGAALDVHESEPFSFAQGPLKDAPNLICTPHTAWYSEQASLEMREAAATEIRRAITGRIPESLRNCVNKEFFVTSAPWSVIDQQAIHPELNGATYRYPPGIVGVAPGGLPAAMEGIIPGGIPVTHNLPTVAHPSQAPSPNQPTKHGDNREHPNEQ

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Dog
100
Xenopus
100
Chicken
100
Sheep
0
Zebrafish
0
Rabbit
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - Q13363/P56545 基板として

Site PTM Type Enzyme
K46 Ubiquitination
C54 S-Nitrosylation
Y76 Phosphorylation
K88 Ubiquitination
S100 Phosphorylation
T144 Phosphorylation
S158 Phosphorylation Q13153 (PAK1) , Q13131 (PRKAA1)
K194 Ubiquitination
R266 Methylation
K273 Ubiquitination
K280 Ubiquitination
R285 Methylation
S300 Phosphorylation
K348 Ubiquitination
C350 S-Nitrosylation
S422 Phosphorylation P45983 (MAPK8) , Q9H2X6 (HIPK2)
K428 Sumoylation
Site PTM Type Enzyme
K6 Acetylation
K8 Acetylation
K10 Acetylation
R22 Methylation
R33 Methylation
K52 Ubiquitination
K94 Ubiquitination
Y108 Phosphorylation
S164 Phosphorylation
K279 Ubiquitination
K286 Ubiquitination
R291 Methylation
S365 Phosphorylation
S369 Phosphorylation
T414 Phosphorylation
S428 Phosphorylation Q9H2X6 (HIPK2)

研究背景

機能:

Corepressor targeting diverse transcription regulators such as GLIS2 or BCL6. Has dehydrogenase activity. Involved in controlling the equilibrium between tubular and stacked structures in the Golgi complex. Functions in brown adipose tissue (BAT) differentiation.

PTMs:

The level of phosphorylation appears to be regulated during the cell cycle. Phosphorylation by HIPK2 on Ser-422 induces proteasomal degradation.

ADP-ribosylated; when cells are exposed to brefeldin A.

Sumoylation on Lys-428 is promoted by the E3 SUMO-protein ligase CBX4.

細胞の位置付け:

Cytoplasm. Nucleus.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Expressed in germinal center B-cells.

サブユニット構造:

Homo- or heterodimer. Heterodimer with CTBP2. Interacts with PRDM16; the interaction represses white adipose tissue (WAT)-specific genes expression. Interacts with GLIS2, FOXP2, HDAC4, HDAC5, HDAC9 and ZNF217. Interacts with ELK3 (via its PXDLS motif). Interacts with RBBP8 (via its PXDLS motif); the interaction is disrupted by binding to adenovirus E1A. Interacts with FOXP1, HIPK2, PNN, NRIP1, MECOM, ZFHX1B and WIZ. Interacts with ZNF366 (via PXDLS motif). Interaction with SATB1 (non-acetylated form); the interaction stabilizes its attachment to DNA and promotes transcription repression. Interacts with BCL6; the interaction is required for BCL6 transcriptional autoinhibition and inhibition of some BCL6 target genes. Interacts with IKZF4 (By similarity). Interacts with MCRIP1 (unphosphorylated form, via the PXDLS motif); competitively inhibiting CTBP-ZEB1 interaction. Interacts with Bassoon/BSN; this interaction targets and anchors CTBP1 to presynapses (By similarity).

(Microbial infection) Interacts with Epstein-Barr virus EBNA3 and EBNA6.

(Microbial infection) Interacts with adenovirus E1A protein (via its C-terminus); the interaction disrupts the interaction of CTBP1 with RBBP8.

(Microbial infection) Interacts with human adenovirus 5 E1A protein; this interaction seems to potentiate viral replication.

タンパク質ファミリー:

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

機能:

Corepressor targeting diverse transcription regulators. Functions in brown adipose tissue (BAT) differentiation (By similarity).

Isoform 2 probably acts as a scaffold for specialized synapses.

細胞の位置付け:

Nucleus. Cell junction>Synapse.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
組織特異性:

Ubiquitous. Highest levels in heart, skeletal muscle, and pancreas.

サブユニット構造:

Can form homodimers or heterodimers of CTBP1 and CTBP2. Interacts with HIPK2 and ZNF217. Interacts with PRDM16; represses white adipose tissue (WAT)-specific genes expression (By similarity). Interacts with PNN, NRIP1 and WIZ. Interacts with MCRIP1.

(Microbial infection) Interacts with human adenovirus 5 E1A protein; this interaction seems to potentiate viral replication.

タンパク質ファミリー:

Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family.

研究領域

· Environmental Information Processing > Signal transduction > Wnt signaling pathway.   (View pathway)

· Environmental Information Processing > Signal transduction > Notch signaling pathway.   (View pathway)

· Human Diseases > Cancers: Overview > Pathways in cancer.   (View pathway)

· Human Diseases > Cancers: Specific types > Chronic myeloid leukemia.   (View pathway)

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