KIF20A Antibody - #AF7664

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製品: KIF20A Antibody
カタログ: AF7664
タンパク質の説明: Rabbit polyclonal antibody to KIF20A
アプリケーション: WB IF/ICC
Cited expt.: WB
反応性: Human
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Xenopus
分子量: 100kDa; 100kD(Calculated).
ユニプロット: O95235
RRID: AB_2844028

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MS Report
HPLC Report
MSDS
説明書
COA
プロトコル
WB Handbook
IHC Protocol
IF/ICC Protocol

製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IF/ICC 1:100-1:500
*The optimal dilutions should be determined by the end user. For optimal experimental results, antibody reuse is not recommended.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(89%), Dog(100%), Xenopus(86%)
クローナリティ:
Polyclonal
特異性:
KIF20A Antibody detects endogenous levels of total KIF20A.
RRID:
AB_2844028
引用形式: Affinity Biosciences Cat# AF7664, RRID:AB_2844028.
コンジュゲート:
Unconjugated.
精製:
The antiserum was purified by peptide affinity chromatography using SulfoLink™ Coupling Resin (Thermo Fisher Scientific).
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ／展開

AA415432; FLJ21151; GG10 2; GG10_2; KI20A_HUMAN; KIF20A; kinesin family member 20A; Kinesin like protein KIF20A; Kinesin-like protein 174; Kinesin-like protein KIF20A; mitotic kinesin like protein 2; Mitotic kinesin-like protein 2; MKlp2; OTTHUMP00000159481; RAB6 interacting, kinesin like (rabkinesin6); Rab6-interacting kinesin-like protein; RAB6-INTERACTING PROTEIN, KINESIN-LIKE; RAB6KIFL; Rabkinesin 6; Rabkinesin-6;

免疫原

免疫原:

A synthesized peptide derived from human KIF20A, corresponding to a region within C-terminal amino acids.

Uniprot:

O95235(KI20A_HUMAN) >>Visit HPA database.

遺伝子(ID):
KIF20A(10112)
タンパク質配列:
MSQGILSPPAGLLSDDDVVVSPMFESTAADLGSVVRKNLLSDCSVVSTSLEDKQQVPSEDSMEKVKVYLRVRPLLPSELERQEDQGCVRIENVETLVLQAPKDSFALKSNERGIGQATHRFTFSQIFGPEVGQASFFNLTVKEMVKDVLKGQNWLIYTYGVTNSGKTHTIQGTIKDGGILPRSLALIFNSLQGQLHPTPDLKPLLSNEVIWLDSKQIRQEEMKKLSLLNGGLQEEELSTSLKRSVYIESRIGTSTSFDSGIAGLSSISQCTSSSQLDETSHRWAQPDTAPLPVPANIRFSIWISFFEIYNELLYDLLEPPSQQRKRQTLRLCEDQNGNPYVKDLNWIHVQDAEEAWKLLKVGRKNQSFASTHLNQNSSRSHSIFSIRILHLQGEGDIVPKISELSLCDLAGSERCKDQKSGERLKEAGNINTSLHTLGRCIAALRQNQQNRSKQNLVPFRDSKLTRVFQGFFTGRGRSCMIVNVNPCASTYDETLHVAKFSAIASQLVHAPPMQLGFPSLHSFIKEHSLQVSPSLEKGAKADTGLDDDIENEADISMYGKEELLQVVEAMKTLLLKERQEKLQLEMHLRDEICNEMVEQMQQREQWCSEHLDTQKELLEEMYEEKLNILKESLTSFYQEEIQERDEKIEELEALLQEARQQSVAHQQSGSELALRRSQRLAASASTQQLQEVKAKLQQCKAELNSTTEELHKYQKMLEPPPSAKPFTIDVDKKLEEGQKNIRLLRTELQKLGESLQSAERACCHSTGAGKLRQALTTCDDILIKQDQTLAELQNNMVLVKLDLRKKAACIAEQYHTVLKLQGQVSAKKRLGTNQENQQPNQQPPGKKPFLRNLLPRTPTCQSSTDCSPYARILRSRRSPLLKSGPFGKKY

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Rabbit
89
Xenopus
86
Zebrafish
57
Chicken
0
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

研究背景

機能:

Mitotic kinesin required for chromosome passenger complex (CPC)-mediated cytokinesis. Following phosphorylation by PLK1, involved in recruitment of PLK1 to the central spindle. Interacts with guanosine triphosphate (GTP)-bound forms of RAB6A and RAB6B. May act as a motor required for the retrograde RAB6 regulated transport of Golgi membranes and associated vesicles along microtubules. Has a microtubule plus end-directed motility.

PTMs:

Phosphorylated by PLK1 at Ser-528 during mitosis, creating a docking site for PLK1 and recruiting PLK1 at central spindle.

細胞の位置付け:

Golgi apparatus. Cytoplasm>Cytoskeleton>Spindle.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
タンパク質ファミリー:

Belongs to the TRAFAC class myosin-kinesin ATPase superfamily. Kinesin family.

参考文献

1). KIF20A is associated with clinical prognosis and synergistic effect of gemcitabine combined with ferroptosis inducer in lung adenocarcinoma. Frontiers in pharmacology, 2022 (PubMed: 36225575) [IF=5.6]

Application: WB    Species: human    Sample: PC9 and A549 cell

FIGURE 7. KIF20A was highly expressed in LUAD cells and regulated the combined effect of GEM and IKE. (A) IHC staining of KIF20A protein in LUAD tissues was analyzed based on the HPA database. (B) Western blot analysis was used to detect KIF20A protein levels in human bronchial epithelial-like cells (16HBE) and LUAD cell lines (H358, PC9, HCC8217, H1299, A549). (C) RT-qPCR for detection of KIF20A mRNA levels in 16HBE and LUAD cell lines. (D) Western blot analysis for detecting knockdown of KIF20A in PC9 and A549. (E) Lipid ROS production was measured by flow cytometry using C11-BODIPY. A549/PC9 cells were treated with IKE for 24 h (F) CCK8 assay to detect the IC50 value of GEM in A549/PC9 after KIF20A knockdown. (G) Colony-forming Assay to assess the effect of KIF20A knockdown on the combined IKE and GEM. n = 3. LUAD: lung adenocarcinoma; GEM: Gemcitabine; IHC: Immunohistochemical; RT-qPCR: Real-Time Quantitative PCR; ROS: reactive oxygen species; IKE: Imidazole Ketone Erastin; IC50: half maximal inhibitory concentration; CCK8 assay: Cell Counting Kit-8 assay; G + I: Gemcitabine + IKE. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns p > 0.05.

Application: IHC    Species: human    Sample: PC9 and A549 cell

FIGURE 7. KIF20A was highly expressed in LUAD cells and regulated the combined effect of GEM and IKE. (A) IHC staining of KIF20A protein in LUAD tissues was analyzed based on the HPA database. (B) Western blot analysis was used to detect KIF20A protein levels in human bronchial epithelial-like cells (16HBE) and LUAD cell lines (H358, PC9, HCC8217, H1299, A549). (C) RT-qPCR for detection of KIF20A mRNA levels in 16HBE and LUAD cell lines. (D) Western blot analysis for detecting knockdown of KIF20A in PC9 and A549. (E) Lipid ROS production was measured by flow cytometry using C11-BODIPY. A549/PC9 cells were treated with IKE for 24 h (F) CCK8 assay to detect the IC50 value of GEM in A549/PC9 after KIF20A knockdown. (G) Colony-forming Assay to assess the effect of KIF20A knockdown on the combined IKE and GEM. n = 3. LUAD: lung adenocarcinoma; GEM: Gemcitabine; IHC: Immunohistochemical; RT-qPCR: Real-Time Quantitative PCR; ROS: reactive oxygen species; IKE: Imidazole Ketone Erastin; IC50: half maximal inhibitory concentration; CCK8 assay: Cell Counting Kit-8 assay; G + I: Gemcitabine + IKE. *p < 0.05; **p < 0.01; ***p < 0.001; ****p < 0.0001; ns p > 0.05.
2). KIF20A Promotes CRC Progression and the Warburg Effect through the C-Myc/HIF-1α Axis. Protein and peptide letters, 2024 (PubMed: 38037834) [IF=1.0]

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