製品: Phospho-Thymidine Kinase 1/TK1 (Tyr181) Antibody
カタログ: AF7178
タンパク質の説明: Rabbit polyclonal antibody to Phospho-Thymidine Kinase 1/TK1 (Tyr181)
アプリケーション: WB IHC
反応性: Human, Mouse, Rat
予測: Pig, Bovine, Horse, Sheep, Rabbit, Dog, Chicken
分子量: 25kDa; 25kD(Calculated).
ユニプロット: P04183
RRID: AB_2843618

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製品説明

ソース:
Rabbit
アプリケーション:
WB 1:500-1:2000, IHC 1:50-1:200
*The optimal dilutions should be determined by the end user.
*Tips:

WB: For western blot detection of denatured protein samples. IHC: For immunohistochemical detection of paraffin sections (IHC-p) or frozen sections (IHC-f) of tissue samples. IF/ICC: For immunofluorescence detection of cell samples. ELISA(peptide): For ELISA detection of antigenic peptide.

反応性:
Human,Mouse,Rat
予測:
Pig(100%), Bovine(100%), Horse(100%), Sheep(100%), Rabbit(100%), Dog(100%), Chicken(100%)
クローナリティ:
Polyclonal
特異性:
Phospho-Thymidine Kinase 1/TK1 (Tyr181) Antibody detects endogenous levels of Thymidine Kinase 1/TK1 only when phosphorylated at Tyr181.
RRID:
AB_2843618
引用形式: Affinity Biosciences Cat# AF7178, RRID:AB_2843618.
コンジュゲート:
Unconjugated.
精製:
The antibody is from purified rabbit serum by affinity purification via sequential chromatography on phospho-peptide and non-phospho-peptide affinity columns.
保存:
Rabbit IgG in phosphate buffered saline , pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol. Store at -20 °C. Stable for 12 months from date of receipt.
別名:

折りたたみ/展開

cytosolic; KITH_HUMAN; Thymidine kinase 1; Thymidine kinase 1 soluble; Thymidine kinase 1 soluble isoform; Thymidine kinase; Thymidine kinase cytosolic; TK 1; TK 2; TK1; Tk1a; Tk1b; TK2;

免疫原

免疫原:
Uniprot:
遺伝子(ID):
タンパク質配列:
MSCINLPTVLPGSPSKTRGQIQVILGPMFSGKSTELMRRVRRFQIAQYKCLVIKYAKDTRYSSSFCTHDRNTMEALPACLLRDVAQEALGVAVIGIDEGQFFPDIVEFCEAMANAGKTVIVAALDGTFQRKPFGAILNLVPLAESVVKLTAVCMECFREAAYTKRLGTEKEVEVIGGADKYHSVCRLCYFKKASGQPAGPDNKENCPVPGKPGEAVAARKLFAPQQILQCSPAN

種類予測

種類予測:

Score>80(red) has high confidence and is suggested to be used for WB detection. *The prediction model is mainly based on the alignment of immunogen sequences, the results are for reference only, not as the basis of quality assurance.

Species
Results
Score
Pig
100
Horse
100
Bovine
100
Sheep
100
Dog
100
Chicken
100
Rabbit
100
Xenopus
78
Zebrafish
78
Model Confidence:
High(score>80) Medium(80>score>50) Low(score<50) No confidence

PTMs - P04183 基板として

Site PTM Type Enzyme
S2 Acetylation
S2 Phosphorylation
T8 Phosphorylation
S13 Phosphorylation P06493 (CDK1) , P24941 (CDK2)
S15 Phosphorylation
K16 Ubiquitination
S30 Phosphorylation
K32 Acetylation
Y48 Phosphorylation
K49 Ubiquitination
Y55 Phosphorylation
S62 Phosphorylation
R70 Methylation
K131 Ubiquitination
K164 Acetylation
K170 Ubiquitination
K180 Ubiquitination
Y181 Phosphorylation
S183 Phosphorylation
K192 Ubiquitination
K203 Ubiquitination
K211 Ubiquitination
K220 Acetylation
K220 Ubiquitination
C230 S-Nitrosylation
S231 Phosphorylation

研究背景

PTMs:

Phosphorylated on Ser-13 in mitosis.

細胞の位置付け:

Cytoplasm.

Extracellular region or secreted Cytosol Plasma membrane Cytoskeleton Lysosome Endosome Peroxisome ER Golgi apparatus Nucleus Mitochondrion Manual annotation Automatic computational assertionSubcellular location
サブユニット構造:

Homotetramer.

タンパク質ファミリー:

Belongs to the thymidine kinase family.

研究領域

· Metabolism > Nucleotide metabolism > Pyrimidine metabolism.

· Metabolism > Xenobiotics biodegradation and metabolism > Drug metabolism - other enzymes.

· Metabolism > Global and overview maps > Metabolic pathways.

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